Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN39_RS02830 Genome accession   NZ_CP141721
Coordinates   574966..575910 (-) Length   314 a.a.
NCBI ID   WP_338997825.1    Uniprot ID   -
Organism   Lactococcus formosensis strain M04020401 EE5e5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569966..580910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN39_RS02795 (VNN39_02795) - 570570..571439 (+) 870 WP_338997824.1 RluA family pseudouridine synthase -
  VNN39_RS02800 (VNN39_02800) - 571526..572371 (-) 846 WP_017368750.1 zinc ABC transporter substrate-binding protein -
  VNN39_RS02805 (VNN39_02805) comGF 572607..573047 (-) 441 WP_017368749.1 competence type IV pilus minor pilin ComGF -
  VNN39_RS02810 (VNN39_02810) comGE 573028..573261 (-) 234 WP_243416060.1 competence type IV pilus minor pilin ComGE -
  VNN39_RS02815 (VNN39_02815) comGD 573293..573709 (-) 417 WP_165711775.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN39_RS02820 (VNN39_02820) comYC 573684..573989 (-) 306 WP_017368746.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN39_RS02825 (VNN39_02825) comGB 574008..575027 (-) 1020 WP_338998085.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN39_RS02830 (VNN39_02830) comGA 574966..575910 (-) 945 WP_338997825.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN39_RS02835 (VNN39_02835) rpsU 576097..576273 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN39_RS02840 (VNN39_02840) guaB 576398..577879 (-) 1482 WP_339007213.1 IMP dehydrogenase -
  VNN39_RS02845 (VNN39_02845) - 578053..579657 (+) 1605 WP_339007215.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35731.91 Da        Isoelectric Point: 6.1725

>NTDB_id=918898 VNN39_RS02830 WP_338997825.1 574966..575910(-) (comGA) [Lactococcus formosensis strain M04020401 EE5e5]
MIQVLAKTLLQKAVDQMVHDLYLVALEGRYSLYFRTATERRFEEEVSLEEGQALIAHMKFLSGMNLGESRRVQLGACTYS
LDKGKQRLRLSTVGDFHGQESMVIRFLHHQQNQLYFWNSELFSTFMGGRGLYLFSGPVGSGKTSLMYKFAREYFKDQQVI
CIEDPVELVEAEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVVAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWQPDEWNGQIDQLVADGFISAATAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918898 VNN39_RS02830 WP_338997825.1 574966..575910(-) (comGA) [Lactococcus formosensis strain M04020401 EE5e5]
ATGATACAGGTTTTGGCAAAAACTCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTACTTAGTAGCATTAGA
AGGGAGGTATTCTCTATATTTTCGTACAGCAACAGAAAGGAGATTTGAAGAAGAAGTATCCCTTGAAGAAGGGCAAGCTC
TGATCGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGGGTACAGTTGGGGGCTTGCACTTATAGC
TTAGATAAAGGTAAGCAACGCTTACGTTTATCTACTGTCGGGGATTTTCATGGTCAAGAAAGTATGGTTATTCGATTCTT
GCATCATCAACAAAACCAACTTTATTTTTGGAACTCAGAGCTTTTTAGCACTTTTATGGGTGGAAGAGGACTTTATCTGT
TTTCAGGACCTGTGGGGTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGAGAGTATTTTAAGGATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTTCAAGTCAATAAAGTTATAGGGAATGATTACGA
TGCCTTGATTAAACTTTCTCTTCGCCATAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCTAAGG
CTGTGCTTCGTGCAAGCTTGACTGGGTATACGGTGTTCTCCACTGTTCATGCACGCTCAATATCGGGTGTAGTTGCACGC
CTAAAAGAACTTGGATTAACTGATTGGGAACTACAATCCAGTCTTCAGCGAGTGATTTATCAGCGATTAATTGCAGGAAA
GGGATTACTTGTTTGTGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

52.532

100

0.529
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

51.438

99.682

0.513
  comGA/cglA/cilD Streptococcus pneumoniae D39

51.438

99.682

0.513
  comGA/cglA/cilD Streptococcus pneumoniae R6

51.438

99.682

0.513
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

51.438

99.682

0.513
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.447

99.045

0.51
  comYA Streptococcus mutans UA159

51.282

99.363

0.51
  comYA Streptococcus mutans UA140

51.282

99.363

0.51