Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN52_RS02905 Genome accession   NZ_CP141701
Coordinates   590347..591291 (-) Length   314 a.a.
NCBI ID   WP_019291447.1    Uniprot ID   -
Organism   Lactococcus petauri strain M18012501 FF2g6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 585347..596291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN52_RS02870 (VNN52_02865) - 585956..586840 (+) 885 WP_019291442.1 RluA family pseudouridine synthase -
  VNN52_RS02875 (VNN52_02870) - 586911..587756 (-) 846 WP_019291443.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  VNN52_RS02880 (VNN52_02875) comGF 587988..588428 (-) 441 WP_019291444.1 competence type IV pilus minor pilin ComGF -
  VNN52_RS02885 (VNN52_02880) comGE 588409..588708 (-) 300 WP_019291445.1 competence type IV pilus minor pilin ComGE -
  VNN52_RS02890 (VNN52_02885) comGD 588674..589090 (-) 417 WP_019291446.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN52_RS02895 (VNN52_02890) comYC 589065..589370 (-) 306 WP_153924350.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN52_RS02900 (VNN52_02895) comGB 589389..590408 (-) 1020 WP_255566838.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN52_RS02905 (VNN52_02900) comGA 590347..591291 (-) 945 WP_019291447.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN52_RS02910 (VNN52_02905) rpsU 591477..591653 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN52_RS02915 (VNN52_02910) guaB 591779..593260 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN52_RS02920 (VNN52_02915) - 593429..595030 (+) 1602 WP_153924348.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35713.01 Da        Isoelectric Point: 6.9700

>NTDB_id=918541 VNN52_RS02905 WP_019291447.1 590347..591291(-) (comGA) [Lactococcus petauri strain M18012501 FF2g6]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTTTERRFEGELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVENEFLQLQVNKVIGNDYDSLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918541 VNN52_RS02905 WP_019291447.1 590347..591291(-) (comGA) [Lactococcus petauri strain M18012501 FF2g6]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAACAACAGAAAGGAGGTTTGAAGGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAAATGAATTTTTGCAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TTCCTTGATTAAGCTTTCGCTGCGACATAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTTCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513