Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN49_RS02795 Genome accession   NZ_CP141699
Coordinates   570319..571263 (-) Length   314 a.a.
NCBI ID   WP_019293012.1    Uniprot ID   -
Organism   Lactococcus petauri strain M17100408 FF4i7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 565319..576263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN49_RS02760 (VNN49_02760) - 565928..566812 (+) 885 WP_019293007.1 RluA family pseudouridine synthase -
  VNN49_RS02765 (VNN49_02765) - 566883..567728 (-) 846 WP_019336001.1 zinc ABC transporter substrate-binding protein -
  VNN49_RS02770 (VNN49_02770) comGF 567960..568400 (-) 441 WP_019293008.1 competence type IV pilus minor pilin ComGF -
  VNN49_RS02775 (VNN49_02775) comGE 568381..568614 (-) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  VNN49_RS02780 (VNN49_02780) comGD 568646..569062 (-) 417 WP_019293010.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN49_RS02785 (VNN49_02785) comYC 569037..569342 (-) 306 WP_029345656.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN49_RS02790 (VNN49_02790) comGB 569361..570380 (-) 1020 WP_256777882.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN49_RS02795 (VNN49_02795) comGA 570319..571263 (-) 945 WP_019293012.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN49_RS02800 (VNN49_02800) rpsU 571449..571625 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN49_RS02805 (VNN49_02805) guaB 571751..573232 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN49_RS02810 (VNN49_02810) - 573401..575002 (+) 1602 WP_019293013.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35700.02 Da        Isoelectric Point: 6.9700

>NTDB_id=918450 VNN49_RS02795 WP_019293012.1 570319..571263(-) (comGA) [Lactococcus petauri strain M17100408 FF4i7]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTTTERRFEGELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDSLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918450 VNN49_RS02795 WP_019293012.1 570319..571263(-) (comGA) [Lactococcus petauri strain M17100408 FF4i7]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAACAACAGAAAGGAGGTTTGAAGGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTGCAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TTCCTTGATTAAGCTTTCGCTGCGACACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTTCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513