Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN49_RS02790 Genome accession   NZ_CP141699
Coordinates   569361..570380 (-) Length   339 a.a.
NCBI ID   WP_256777882.1    Uniprot ID   -
Organism   Lactococcus petauri strain M17100408 FF4i7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 564361..575380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN49_RS02760 (VNN49_02760) - 565928..566812 (+) 885 WP_019293007.1 RluA family pseudouridine synthase -
  VNN49_RS02765 (VNN49_02765) - 566883..567728 (-) 846 WP_019336001.1 zinc ABC transporter substrate-binding protein -
  VNN49_RS02770 (VNN49_02770) comGF 567960..568400 (-) 441 WP_019293008.1 competence type IV pilus minor pilin ComGF -
  VNN49_RS02775 (VNN49_02775) comGE 568381..568614 (-) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  VNN49_RS02780 (VNN49_02780) comGD 568646..569062 (-) 417 WP_019293010.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN49_RS02785 (VNN49_02785) comYC 569037..569342 (-) 306 WP_029345656.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN49_RS02790 (VNN49_02790) comGB 569361..570380 (-) 1020 WP_256777882.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN49_RS02795 (VNN49_02795) comGA 570319..571263 (-) 945 WP_019293012.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN49_RS02800 (VNN49_02800) rpsU 571449..571625 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN49_RS02805 (VNN49_02805) guaB 571751..573232 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN49_RS02810 (VNN49_02810) - 573401..575002 (+) 1602 WP_019293013.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39510.19 Da        Isoelectric Point: 9.5027

>NTDB_id=918449 VNN49_RS02790 WP_256777882.1 569361..570380(-) (comGB) [Lactococcus petauri strain M17100408 FF4i7]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGKSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRKKLVQVATYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTL
FLGWLFSLFVFFYLCRYYWKKKTALEALRLMVKIPFVGRFVQLYLTAYFAREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDSGQKFEDALRFYPIFTKELSLIVEYGELKSKLGKELMIFSEENWSLFFERMERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918449 VNN49_RS02790 WP_256777882.1 569361..570380(-) (comGB) [Lactococcus petauri strain M17100408 FF4i7]
ATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAAGCTCATTCAACTCATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTCTCTGAAATTATTGATTTCCTTGAACGATCGGGTCTTACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTTAATGGGAAAAGTTTCTCTGGTATTTTGGCGAAGCTAAATTTTTATGAGGATGTT
GTGACTCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTATACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAAAAAGCTTGTCCAAGTTGCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATTATGCTTG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCCTC
TTTTTAGGGTGGCTCTTCAGTTTGTTCGTTTTCTTTTATCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GCTGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTGCAAGAGAATGGG
GAAACTTAATTGCTCAAGCTGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATTTTCAAAGAA
TTCGGTTTTGAGCTTCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCTCTTCGATTTTACCCCATATTTACCAA
GGAGCTCTCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTAATGATTTTTTCTGAAGAAAACT
GGTCGCTCTTTTTTGAAAGGATGGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCGGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.976

99.115

0.525
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comYB Streptococcus gordonii str. Challis substr. CH1

52.395

98.525

0.516
  comYB Streptococcus mutans UA140

52.548

92.625

0.487
  comYB Streptococcus mutans UA159

52.229

92.625

0.484