Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABYA78_RS27255 Genome accession   NZ_CP159883
Coordinates   5707584..5708048 (-) Length   154 a.a.
NCBI ID   WP_062838232.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 100690     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5702584..5713048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABYA78_RS27240 (ABYA78_27240) nadC 5705084..5705932 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABYA78_RS27250 (ABYA78_27250) - 5706113..5707426 (-) 1314 WP_015648064.1 O-antigen ligase family protein -
  ABYA78_RS27255 (ABYA78_27255) pilA/pilAI 5707584..5708048 (-) 465 WP_062838232.1 pilin Machinery gene
  ABYA78_RS27260 (ABYA78_27260) pilB 5708279..5709979 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABYA78_RS27265 (ABYA78_27265) pilC 5709983..5711200 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  ABYA78_RS27270 (ABYA78_27270) pilD 5711201..5712073 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ABYA78_RS27275 (ABYA78_27275) coaE 5712070..5712681 (+) 612 WP_033982615.1 dephospho-CoA kinase -
  ABYA78_RS27280 (ABYA78_27280) yacG 5712678..5712878 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16179.48 Da        Isoelectric Point: 8.9986

>NTDB_id=915575 ABYA78_RS27255 WP_062838232.1 5707584..5708048(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 100690]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCQKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=915575 ABYA78_RS27255 WP_062838232.1 5707584..5708048(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 100690]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCAGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

36.257

100

0.403
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.584

96.753

0.364
  pilA2 Legionella pneumophila strain ERS1305867

37.584

96.753

0.364