Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U3Q39_RS04785 Genome accession   NZ_CP140298
Coordinates   1004437..1006140 (+) Length   567 a.a.
NCBI ID   WP_170799084.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain MJL19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 999437..1011140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3Q39_RS04765 (U3Q39_004765) - 1000652..1001548 (-) 897 WP_170799081.1 ABC transporter ATP-binding protein -
  U3Q39_RS04770 (U3Q39_004770) - 1001545..1002171 (-) 627 WP_170799082.1 hypothetical protein -
  U3Q39_RS04775 (U3Q39_004775) - 1002383..1003183 (-) 801 WP_241694827.1 hypothetical protein -
  U3Q39_RS04780 (U3Q39_004780) pilA 1003816..1004001 (-) 186 WP_170799083.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  U3Q39_RS04785 (U3Q39_004785) pilB 1004437..1006140 (+) 1704 WP_170799084.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U3Q39_RS04790 (U3Q39_004790) pilC 1006143..1007360 (+) 1218 WP_095460666.1 type II secretion system F family protein Machinery gene
  U3Q39_RS04795 (U3Q39_004795) pilD 1007366..1008232 (+) 867 WP_170799085.1 prepilin peptidase Machinery gene
  U3Q39_RS04800 (U3Q39_004800) coaE 1008364..1008972 (+) 609 WP_170799086.1 dephospho-CoA kinase -
  U3Q39_RS04805 (U3Q39_004805) yacG 1008969..1009166 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  U3Q39_RS04810 (U3Q39_004810) - 1009179..1009859 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  U3Q39_RS04815 (U3Q39_004815) - 1009916..1010956 (-) 1041 WP_170799087.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62169.94 Da        Isoelectric Point: 5.4513

>NTDB_id=913589 U3Q39_RS04785 WP_170799084.1 1004437..1006140(+) (pilB) [Stutzerimonas stutzeri strain MJL19]
MNENVPLGGLARQLVQAGQLDEKTAQQAQQQALRNQVPLVTWLVQNKLVKGRALVGIAGEQFGVPYFDLGSLDKEAQPRD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGETIDKFFESAASSLDGLHDSELD
GLDIEEAGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSSKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLSEGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=913589 U3Q39_RS04785 WP_170799084.1 1004437..1006140(+) (pilB) [Stutzerimonas stutzeri strain MJL19]
ATGAATGAAAACGTGCCCCTCGGTGGCTTGGCTCGCCAGCTGGTGCAGGCCGGCCAGCTCGATGAGAAGACAGCGCAGCA
AGCCCAGCAACAGGCGCTGCGTAATCAGGTCCCGTTGGTAACCTGGCTGGTGCAGAACAAGCTTGTCAAAGGGCGTGCTC
TGGTGGGCATCGCGGGCGAGCAGTTCGGCGTGCCGTATTTCGATCTCGGATCGCTCGACAAGGAGGCTCAGCCAAGAGAT
CTGGTGAGCGAGAAGCTGGTGCGCCAGCATCGGGCGTTGCCGCTGCAGCGGCGAGGCAACAAGCTTTATGTGGCTGTTTC
GGACCCAACCAATCATCAGGCTATCAGCGATATTCAGTTCAATACTGGCCTCTCAGTCGAGGCTGTACTGGTTGAGGATG
ACAAGCTCGGCGAAACGATTGACAAATTCTTCGAATCGGCAGCCTCATCCCTTGATGGGTTGCATGATTCCGAGCTGGAC
GGCTTGGATATCGAAGAGGCAGGCGATGCCAAGGAGGACGATGCGGCACATAACGAAGCCGATGATGCGCCAGTGGTGCG
GTTTGTTAACAAGATGTTGCTCGATGCGATCAAGGGTGGTTCGTCCGATTTGCACTTCGAACCCTATGAGAAGAGCTATC
GGGTGCGCTTTCGTACGGATGGCATGCTGCACGAGGTTGCCCGCCCTCCGGTCCAGTTGGTCAGCCGTATTTCTGCGCGT
CTGAAAGTAATGGCCAGTCTGGATATCGCCGAGCGGCGCAAGCCGCAGGATGGGCGAATCAAGATGAAGGTGTCGAGCTC
CAAGTCCATTGATTTTCGCGTCAATACGCTACCCACCTTGTGGGGCGAGAAGATCGTCATGCGAATCTTAGATTCCTCAA
GTGCCAAGATGGGGATCGATGCGCTGGGATACGAGGATGACCAGAAGGAGCTTTATCTTAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGTCCGACAGGCTCGGGTAAGACCGTTTCGCTTTATACCGGCCTAAATATCCTCAATACGGT
CGATGTGAATATTTCCACCGCTGAAGATCCGGTTGAAATCAATTTGGAGGGGATCAATCAGGTCAACGTAAATCCTAAGC
AGGGCATGGATTTCGCGCAGGCGCTTCGGGCATTTCTACGCCAGGATCCTGACATCATCATGGTAGGTGAGATCCGAGAT
CTCGAAACGGCTGAGATTGCCATCAAGGCAGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACTCGCTTGCGCAACATGGGGGTGCCGGCATTCAACATTGCCACATCGGTCAACCTGATCATTGCTCAAC
GGCTGGCTCGTAAGCTGTGCCCCAGCTGTAAACAAGAAGTCGATGTGCCGCGAGATGTTCTGCTCAGCGAAGGTTTTCCT
GAAGCGCGAATCGGTTCCTTCAAGCTTTACGGGCCGGTGGGGTGCGATAGCTGCAAAGGAGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCTCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAAAGATGGGTTCAATGATCTGCGCACTTCGGGTTTGGTCAAGGCCATGAATGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.676

99.118

0.601
  pilB Acinetobacter baylyi ADP1

58.584

99.647

0.584
  pilB Legionella pneumophila strain ERS1305867

55.088

100

0.554
  pilF Neisseria gonorrhoeae MS11

50.088

99.647

0.499
  pilB Vibrio campbellii strain DS40M4

49.734

99.295

0.494
  pilB Vibrio cholerae strain A1552

53.922

89.947

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

53.057

89.418

0.474
  pilF Thermus thermophilus HB27

38.204

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

93.298

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362