Detailed information NTG0091248

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: L6411_RS06765
• Genome accession: NZ_AP025333
• Coordinates: 1400016..1400672 (-)
• Length: 218 a.a.
• NCBI ID: WP_024532637.1
• Uniprot ID: -
• Organism: Streptococcus ruminantium strain GUT-189
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1395016..1405672

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6411_RS06745 (GUT189_12940)	sodA	1395043..1395654 (-)	612	WP_024532641.1	superoxide dismutase SodA	-
L6411_RS06750 (GUT189_12950)	holA	1395751..1396782 (-)	1032	WP_237373948.1	DNA polymerase III subunit delta	-
L6411_RS06755 (GUT189_12960)	-	1397038..1397652 (-)	615	WP_227990611.1	hypothetical protein	-
L6411_RS06760 (GUT189_12970)	comEC/celB	1397798..1400032 (-)	2235	WP_237373949.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
L6411_RS06765 (GUT189_12980)	comEA	1400016..1400672 (-)	657	WP_024532637.1	helix-hairpin-helix domain-containing protein	Machinery gene
L6411_RS06770 (GUT189_12990)	-	1400757..1401500 (-)	744	WP_024532636.1	lysophospholipid acyltransferase family protein	-
L6411_RS06775 (GUT189_13000)	-	1401691..1403136 (+)	1446	WP_156021108.1	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase	-
L6411_RS06780 (GUT189_13010)	-	1403496..1404284 (+)	789	WP_024532634.1	ABC transporter ATP-binding protein	-
L6411_RS06785 (GUT189_13020)	-	1404294..1405223 (+)	930	WP_237373950.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23008.77 Da
• Isoelectric Point: 4.4459

>NTDB_id=91248 L6411_RS06765 WP_024532637.1 1400016..1400672(-) (comEA) [Streptococcus ruminantium strain GUT-189]
MEKVIECIKEYKWQVGLPVIAVVVVASFLLFRQSAQPVDQTGLTKFSQTEQISSSQEVIEETSTEASEKNSKLVVDVKGA
VINPGLYTLETGARINDAIAAAGGFTSQADQKSINLAQKLTDETVVYVASQGENISVVPNSSTSSALSQEGNKSGLVNLN
TATVADLQTISGIGAKRAADIIAYREANGSFKSVDDLSNVSGIGDKTLESIRSYVTVG

Nucleotide

• Length: 657 bp

>NTDB_id=91248 L6411_RS06765 WP_024532637.1 1400016..1400672(-) (comEA) [Streptococcus ruminantium strain GUT-189]
ATGGAAAAAGTTATTGAATGCATTAAAGAATACAAGTGGCAAGTTGGTCTGCCTGTGATAGCAGTAGTGGTGGTGGCGAG
TTTTCTTCTTTTTAGACAGTCAGCTCAGCCAGTTGACCAGACAGGATTGACCAAGTTTTCACAGACAGAACAAATCAGCT
CCAGTCAAGAAGTGATAGAAGAGACCTCAACAGAAGCTAGCGAGAAAAACAGCAAACTAGTGGTGGATGTCAAGGGTGCA
GTGATAAATCCCGGTCTTTACACCTTAGAGACTGGTGCCCGTATCAATGATGCCATTGCCGCAGCGGGCGGTTTCACTAG
TCAAGCAGATCAAAAATCTATCAATCTAGCGCAAAAGCTGACTGATGAAACAGTCGTCTATGTGGCTAGTCAGGGTGAGA
ATATTTCGGTAGTTCCCAATTCTTCAACTAGCTCAGCCCTGTCTCAAGAGGGAAATAAATCTGGTCTAGTTAATCTGAAT
ACAGCTACGGTGGCGGACTTACAGACCATTTCAGGCATCGGTGCTAAGCGAGCAGCGGATATTATTGCTTATCGTGAAGC
CAATGGTTCCTTCAAGTCGGTGGACGATCTCAGCAATGTTTCTGGCATTGGTGACAAGACCCTAGAAAGTATCCGCTCCT
ATGTTACGGTTGGATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (74-128)

• Helix-hairpin-helix motif (HHH_3) (155-216)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.807	100	0.5
comEA/celA/cilE	Streptococcus pneumoniae Rx1	46.637	100	0.477
comEA/celA/cilE	Streptococcus pneumoniae D39	46.637	100	0.477
comEA/celA/cilE	Streptococcus pneumoniae R6	46.637	100	0.477
comEA/celA/cilE	Streptococcus mitis NCTC 12261	46.637	100	0.477
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	46.119	100	0.463
comEA/celA/cilE	Streptococcus mitis SK321	44.495	100	0.445
comEA	Lactococcus lactis subsp. cremoris KW2	41.441	100	0.422
comEA	Bacillus subtilis subsp. subtilis str. 168	42.632	87.156	0.372