Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   L6411_RS00790 Genome accession   NZ_AP025333
Coordinates   123947..124978 (+) Length   343 a.a.
NCBI ID   WP_272877484.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-189     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 118947..129978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6411_RS00775 (GUT189_01220) - 121559..121927 (+) 369 WP_155962601.1 DUF1033 family protein -
  L6411_RS00780 (GUT189_01230) - 121965..122915 (-) 951 WP_155974247.1 S66 family peptidase -
  L6411_RS00785 (GUT189_01240) comYA 123076..124026 (+) 951 WP_155962599.1 competence type IV pilus ATPase ComGA Machinery gene
  L6411_RS00790 (GUT189_01250) comYB 123947..124978 (+) 1032 WP_272877484.1 competence type IV pilus assembly protein ComGB Machinery gene
  L6411_RS00795 (GUT189_01260) comYC 124975..125256 (+) 282 WP_237374201.1 competence type IV pilus major pilin ComGC Machinery gene
  L6411_RS00800 (GUT189_01270) comGD 125243..125656 (+) 414 WP_237374202.1 competence type IV pilus minor pilin ComGD -
  L6411_RS00805 (GUT189_01280) comYE 125628..125921 (+) 294 WP_155963927.1 competence type IV pilus minor pilin ComGE Machinery gene
  L6411_RS00810 (GUT189_01290) comGF/cglF 125908..126342 (+) 435 WP_237374203.1 competence type IV pilus minor pilin ComGF Machinery gene
  L6411_RS00815 (GUT189_01300) comGG 126320..126733 (+) 414 WP_120171207.1 competence type IV pilus minor pilin ComGG -
  L6411_RS00820 (GUT189_01310) comYH 126738..127691 (+) 954 WP_237374204.1 class I SAM-dependent methyltransferase Machinery gene
  L6411_RS00825 (GUT189_01320) - 127741..128928 (+) 1188 WP_024532248.1 acetate kinase -
  L6411_RS00830 (GUT189_01330) - 129233..129787 (+) 555 WP_024532247.1 folate family ECF transporter S component -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 39014.94 Da        Isoelectric Point: 8.3881

>NTDB_id=91220 L6411_RS00790 WP_272877484.1 123947..124978(+) (comYB) [Streptococcus ruminantium strain GUT-189]
MIAFLQQDISVIDRQRQKKLSLDHQRKVIELFNNLFASGFHLGEIVDFLRRSQLLADRYTQVLSDGLLAGKPFSSLLADL
RFSDAVVTQVSLAEVHGNTSLSLFHIQSYLENVSKIRKKLIEVATYPVILLAFLLLIVVGLKNYLLPQLEEENIATVLIN
HLPAIFLSVVFLLSVGVLAGLTCYRKASKIKVFSRLVALPFLGKVIQTYLTAYYAREWGSLIGQGLEMPQIVELMQTQRS
QLFREIGEDLESALSNGQSFHYHIQSYPFFKRELSLIIEYGQVKSKLGSELALYAAECWEEFFSRINKAMQLIQPLVFLF
VALMVVLIYAAMLLPIYQNMELK

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=91220 L6411_RS00790 WP_272877484.1 123947..124978(+) (comYB) [Streptococcus ruminantium strain GUT-189]
TTGATAGCCTTTTTGCAGCAGGATATATCAGTCATAGACAGGCAGAGACAGAAAAAATTGTCTTTGGATCACCAGCGTAA
GGTGATTGAATTATTTAACAATCTCTTTGCAAGTGGTTTTCATTTGGGAGAAATTGTGGATTTTCTCAGGCGCAGTCAGC
TCCTGGCGGACCGTTACACACAGGTTTTATCAGATGGACTGTTGGCTGGTAAACCATTTTCTAGCCTTCTAGCGGATCTT
CGTTTTTCGGATGCTGTTGTCACTCAGGTTTCTCTTGCAGAAGTTCATGGTAATACTAGTTTGAGTCTGTTCCACATCCA
GTCCTATCTGGAAAATGTCAGTAAAATCCGTAAAAAATTGATCGAGGTTGCCACTTATCCAGTCATTTTACTGGCTTTTC
TTCTCCTGATTGTAGTAGGCTTGAAAAACTATCTCTTGCCGCAATTGGAAGAAGAAAACATAGCAACGGTGTTGATTAAC
CACTTACCAGCTATTTTTTTATCCGTAGTTTTCTTATTATCGGTAGGAGTTTTAGCAGGTCTTACTTGCTATCGCAAAGC
GAGCAAAATCAAGGTCTTTTCACGCTTGGTTGCTTTGCCATTTTTGGGAAAAGTTATCCAAACCTATCTAACAGCGTATT
ATGCTAGGGAGTGGGGAAGTCTAATCGGTCAGGGCTTAGAAATGCCACAGATTGTGGAGTTGATGCAGACACAGCGGTCG
CAATTATTCAGAGAGATTGGAGAAGATTTAGAATCAGCTCTTTCAAATGGTCAGAGCTTCCATTATCATATTCAGTCCTA
TCCATTTTTCAAGCGAGAGTTGAGTTTGATTATTGAGTATGGACAAGTCAAATCCAAACTGGGAAGCGAGTTGGCGTTGT
ATGCAGCGGAGTGTTGGGAAGAATTTTTCTCCCGAATCAATAAAGCCATGCAGTTGATTCAACCGCTAGTCTTTCTCTTT
GTAGCCTTGATGGTCGTTCTTATCTACGCAGCCATGTTGCTGCCTATTTATCAGAATATGGAGTTAAAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(30-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus gordonii str. Challis substr. CH1

62.865

99.708

0.627
  comGB/cglB Streptococcus mitis NCTC 12261

60.714

97.959

0.595
  comYB Streptococcus mutans UA159

59.012

100

0.592
  comYB Streptococcus mutans UA140

59.012

100

0.592
  comGB/cglB Streptococcus pneumoniae Rx1

59.524

97.959

0.583
  comGB/cglB Streptococcus pneumoniae R6

59.524

97.959

0.583
  comGB/cglB Streptococcus pneumoniae TIGR4

59.524

97.959

0.583
  comGB/cglB Streptococcus pneumoniae D39

59.524

97.959

0.583
  comGB/cglB Streptococcus mitis SK321

59.226

97.959

0.58
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

98.251

0.493

Multiple sequence alignment