Detailed information NTG0091223

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: L6411_RS00810
• Genome accession: NZ_AP025333
• Coordinates: 125908..126342 (+)
• Length: 144 a.a.
• NCBI ID: WP_237374203.1
• Uniprot ID: -
• Organism: Streptococcus ruminantium strain GUT-189
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 120908..131342

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6411_RS00775 (GUT189_01220)	-	121559..121927 (+)	369	WP_155962601.1	DUF1033 family protein	-
L6411_RS00780 (GUT189_01230)	-	121965..122915 (-)	951	WP_155974247.1	S66 family peptidase	-
L6411_RS00785 (GUT189_01240)	comYA	123076..124026 (+)	951	WP_155962599.1	competence type IV pilus ATPase ComGA	Machinery gene
L6411_RS00790 (GUT189_01250)	comYB	123947..124978 (+)	1032	WP_272877484.1	competence type IV pilus assembly protein ComGB	Machinery gene
L6411_RS00795 (GUT189_01260)	comYC	124975..125256 (+)	282	WP_237374201.1	competence type IV pilus major pilin ComGC	Machinery gene
L6411_RS00800 (GUT189_01270)	comGD	125243..125656 (+)	414	WP_237374202.1	competence type IV pilus minor pilin ComGD	-
L6411_RS00805 (GUT189_01280)	comYE	125628..125921 (+)	294	WP_155963927.1	competence type IV pilus minor pilin ComGE	Machinery gene
L6411_RS00810 (GUT189_01290)	comGF/cglF	125908..126342 (+)	435	WP_237374203.1	competence type IV pilus minor pilin ComGF	Machinery gene
L6411_RS00815 (GUT189_01300)	comGG	126320..126733 (+)	414	WP_120171207.1	competence type IV pilus minor pilin ComGG	-
L6411_RS00820 (GUT189_01310)	comYH	126738..127691 (+)	954	WP_237374204.1	class I SAM-dependent methyltransferase	Machinery gene
L6411_RS00825 (GUT189_01320)	-	127741..128928 (+)	1188	WP_024532248.1	acetate kinase	-
L6411_RS00830 (GUT189_01330)	-	129233..129787 (+)	555	WP_024532247.1	folate family ECF transporter S component	-
L6411_RS00835 (GUT189_01340)	-	129886..131142 (+)	1257	WP_237374205.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16455.91 Da
• Isoelectric Point: 8.8413

>NTDB_id=91223 L6411_RS00810 WP_237374203.1 125908..126342(+) (comGF/cglF) [Streptococcus ruminantium strain GUT-189]
MLKTRAPAFTLLECLVALVVLSGSLLVFEGLSKLISHEVRYQSKVLQKDWLLFSDQLRAEWAQAALVRVENNRIYINKEG
QGLAFGKSRSDDFRKTNEKGQGYQPILYGLQATEIVQEGRLVRMDFTFTNGEERTFIYAFEKTG

Nucleotide

• Length: 435 bp

>NTDB_id=91223 L6411_RS00810 WP_237374203.1 125908..126342(+) (comGF/cglF) [Streptococcus ruminantium strain GUT-189]
TTGCTAAAAACTAGGGCTCCAGCTTTTACCCTTTTAGAATGTTTAGTGGCTTTGGTCGTCCTGTCGGGGAGTCTCTTAGT
ATTTGAAGGATTAAGCAAATTGATTTCTCATGAAGTTCGTTACCAAAGCAAGGTGCTTCAAAAGGATTGGCTCCTTTTCT
CGGATCAGCTGCGTGCAGAATGGGCTCAGGCGGCTTTGGTTAGAGTTGAGAATAACAGAATCTACATCAATAAGGAAGGT
CAAGGTCTTGCCTTTGGAAAATCACGTTCGGATGATTTTCGGAAGACGAATGAGAAGGGGCAAGGGTATCAGCCTATATT
ATACGGTCTTCAAGCGACGGAAATTGTTCAAGAGGGAAGACTAGTTAGAATGGATTTTACCTTTACAAATGGAGAGGAGC
GAACCTTTATCTATGCTTTTGAAAAAACAGGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (40-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	52.518	96.528	0.507
comGF/cglF	Streptococcus pneumoniae Rx1	52.174	95.833	0.5
comGF/cglF	Streptococcus pneumoniae D39	52.174	95.833	0.5
comGF/cglF	Streptococcus pneumoniae R6	52.174	95.833	0.5
comGF/cglF	Streptococcus pneumoniae TIGR4	52.174	95.833	0.5
comGF/cglF	Streptococcus mitis SK321	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	49.645	97.917	0.486
comYF	Streptococcus mutans UA140	48.936	97.917	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	45.07	98.611	0.444