Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   L6411_RS00785 Genome accession   NZ_AP025333
Coordinates   123076..124026 (+) Length   316 a.a.
NCBI ID   WP_155962599.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-189     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 118076..129026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6411_RS00775 (GUT189_01220) - 121559..121927 (+) 369 WP_155962601.1 DUF1033 family protein -
  L6411_RS00780 (GUT189_01230) - 121965..122915 (-) 951 WP_155974247.1 S66 family peptidase -
  L6411_RS00785 (GUT189_01240) comYA 123076..124026 (+) 951 WP_155962599.1 competence type IV pilus ATPase ComGA Machinery gene
  L6411_RS00790 (GUT189_01250) comYB 123947..124978 (+) 1032 WP_272877484.1 competence type IV pilus assembly protein ComGB Machinery gene
  L6411_RS00795 (GUT189_01260) comYC 124975..125256 (+) 282 WP_237374201.1 competence type IV pilus major pilin ComGC Machinery gene
  L6411_RS00800 (GUT189_01270) comGD 125243..125656 (+) 414 WP_237374202.1 competence type IV pilus minor pilin ComGD -
  L6411_RS00805 (GUT189_01280) comYE 125628..125921 (+) 294 WP_155963927.1 competence type IV pilus minor pilin ComGE Machinery gene
  L6411_RS00810 (GUT189_01290) comGF/cglF 125908..126342 (+) 435 WP_237374203.1 competence type IV pilus minor pilin ComGF Machinery gene
  L6411_RS00815 (GUT189_01300) comGG 126320..126733 (+) 414 WP_120171207.1 competence type IV pilus minor pilin ComGG -
  L6411_RS00820 (GUT189_01310) comYH 126738..127691 (+) 954 WP_237374204.1 class I SAM-dependent methyltransferase Machinery gene
  L6411_RS00825 (GUT189_01320) - 127741..128928 (+) 1188 WP_024532248.1 acetate kinase -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 35633.60 Da        Isoelectric Point: 6.6183

>NTDB_id=91219 L6411_RS00785 WP_155962599.1 123076..124026(+) (comYA) [Streptococcus ruminantium strain GUT-189]
MIQEKARQMVEQAVLARVSDLYLVPRGESYQLYHRIMDERVFLQELNEEEVTAIISHFKFLAGLNVGEKRRSQQGSCDYN
YGSGQISLRLSTVGDYRGKESLVIRLLYDNDKELKFWFEAAERIAREIKGRGLYLFSGPVGSGKTTLMYHLARLKFPDKQ
ILTIEDPVEIKQEDMLQLQLNEAIGATYDNLIKLSLRHRPDLLIIGEIRDAETARAVIRASLTGATVFSTVHARSISGVY
ARMLELGVSTEELNNALQGIAYQRLIGGGGVVDFAKENFQNHSADQWNKQIDSLFAAGYISHRQAETEKIVFGSPA

Nucleotide


Download         Length: 951 bp        

>NTDB_id=91219 L6411_RS00785 WP_155962599.1 123076..124026(+) (comYA) [Streptococcus ruminantium strain GUT-189]
ATGATTCAAGAAAAAGCAAGACAGATGGTGGAGCAAGCAGTGCTTGCTAGGGTTAGCGACCTTTATTTGGTTCCGCGAGG
GGAGTCCTATCAGCTCTATCATCGGATTATGGATGAACGAGTATTTTTGCAGGAATTGAATGAAGAAGAAGTGACAGCCA
TCATCAGTCATTTCAAGTTTTTGGCTGGTTTGAATGTCGGTGAAAAGCGTCGCAGTCAGCAAGGTTCTTGTGATTATAAT
TATGGTTCAGGGCAGATCTCCCTCCGCTTGTCCACTGTTGGTGATTACCGAGGTAAGGAGAGTTTGGTGATTCGATTGCT
GTACGATAACGATAAGGAACTCAAGTTTTGGTTTGAGGCAGCAGAGCGGATTGCTAGGGAAATCAAGGGGCGAGGACTCT
ACCTTTTTTCGGGTCCGGTCGGCTCTGGCAAAACCACGCTTATGTACCATCTTGCTAGGCTGAAATTCCCAGATAAACAG
ATTTTGACGATTGAAGATCCGGTAGAAATCAAACAGGAAGATATGCTCCAACTTCAGCTCAATGAAGCGATTGGGGCAAC
GTATGACAATCTGATTAAGCTGTCTCTTCGTCATCGGCCGGATTTACTGATTATTGGGGAAATACGAGATGCAGAAACAG
CAAGGGCAGTCATTCGTGCTAGTCTGACAGGTGCAACAGTTTTTTCTACTGTCCATGCTCGCAGTATTTCAGGGGTCTAT
GCTCGGATGCTAGAGCTGGGTGTCAGTACAGAGGAGTTAAATAATGCCCTTCAAGGAATTGCCTATCAACGTTTGATTGG
GGGAGGGGGTGTTGTAGATTTTGCTAAAGAAAACTTCCAAAACCATTCTGCTGACCAGTGGAATAAACAGATTGATAGCC
TTTTTGCAGCAGGATATATCAGTCATAGACAGGCAGAGACAGAAAAAATTGTCTTTGGATCACCAGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

67.742

98.101

0.665
  comYA Streptococcus mutans UA159

67.524

98.418

0.665
  comYA Streptococcus mutans UA140

67.524

98.418

0.665
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.782

98.734

0.63
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.782

98.734

0.63
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.782

98.734

0.63
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.782

98.734

0.63
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

63.462

98.734

0.627
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.871

98.101

0.627
  comGA Lactococcus lactis subsp. cremoris KW2

54.019

98.418

0.532
  comGA Latilactobacillus sakei subsp. sakei 23K

42.599

87.658

0.373

Multiple sequence alignment