Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   L6419_RS04035 Genome accession   NZ_AP025332
Coordinates   809095..809751 (+) Length   218 a.a.
NCBI ID   WP_024532637.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-184     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 804095..814751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6419_RS04015 (GUT184_07440) - 804545..805474 (-) 930 WP_120171917.1 ABC transporter substrate-binding protein -
  L6419_RS04020 (GUT184_07450) - 805484..806272 (-) 789 WP_024532634.1 ABC transporter ATP-binding protein -
  L6419_RS04025 (GUT184_07460) - 806631..808076 (-) 1446 WP_155974169.1 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase -
  L6419_RS04030 (GUT184_07470) - 808267..809010 (+) 744 WP_024532636.1 lysophospholipid acyltransferase family protein -
  L6419_RS04035 (GUT184_07480) comEA 809095..809751 (+) 657 WP_024532637.1 helix-hairpin-helix domain-containing protein Machinery gene
  L6419_RS04040 (GUT184_07490) comEC/celB 809735..811969 (+) 2235 WP_237375687.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  L6419_RS04045 (GUT184_07500) - 812116..812727 (+) 612 WP_228025421.1 hypothetical protein -
  L6419_RS04050 (GUT184_07510) holA 812983..814014 (+) 1032 WP_237375688.1 DNA polymerase III subunit delta -
  L6419_RS04055 (GUT184_07520) sodA 814111..814722 (+) 612 WP_024532641.1 superoxide dismutase SodA -

Sequence

Protein


Download         Length: 218 a.a.        Molecular weight: 23008.77 Da        Isoelectric Point: 4.4459

>NTDB_id=91183 L6419_RS04035 WP_024532637.1 809095..809751(+) (comEA) [Streptococcus ruminantium strain GUT-184]
MEKVIECIKEYKWQVGLPVIAVVVVASFLLFRQSAQPVDQTGLTKFSQTEQISSSQEVIEETSTEASEKNSKLVVDVKGA
VINPGLYTLETGARINDAIAAAGGFTSQADQKSINLAQKLTDETVVYVASQGENISVVPNSSTSSALSQEGNKSGLVNLN
TATVADLQTISGIGAKRAADIIAYREANGSFKSVDDLSNVSGIGDKTLESIRSYVTVG

Nucleotide


Download         Length: 657 bp        

>NTDB_id=91183 L6419_RS04035 WP_024532637.1 809095..809751(+) (comEA) [Streptococcus ruminantium strain GUT-184]
ATGGAAAAAGTTATTGAATGCATTAAAGAATACAAGTGGCAAGTTGGTCTGCCTGTGATAGCAGTAGTGGTGGTGGCGAG
TTTTCTTCTTTTTAGACAGTCAGCTCAACCAGTTGACCAGACAGGATTGACCAAGTTTTCACAGACAGAACAAATCAGCT
CCAGTCAAGAAGTGATAGAAGAGACCTCAACAGAAGCTAGCGAGAAAAACAGCAAACTAGTGGTGGATGTCAAGGGTGCA
GTGATAAATCCCGGTCTTTACACCTTAGAGACTGGTGCCCGTATCAATGATGCTATTGCCGCAGCGGGCGGTTTCACTAG
TCAAGCAGATCAAAAATCTATCAATCTAGCACAAAAACTGACTGATGAAACAGTCGTCTATGTGGCTAGTCAGGGTGAGA
ATATTTCGGTAGTTCCCAATTCTTCAACTAGCTCAGCCCTGTCTCAAGAGGGAAATAAATCTGGTCTAGTTAATCTGAAT
ACAGCTACGGTGGCGGACTTACAGACCATTTCAGGCATCGGTGCTAAGCGAGCAGCGGATATTATTGCTTATCGTGAAGC
CAATGGTTCCTTCAAGTCGGTGGACGATCTCAGCAATGTTTCTGGCATTGGTGACAAGACCCTAGAAAGTATCCGCTCCT
ATGTTACGGTTGGATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(74-128)

Helix-hairpin-helix motif (HHH_3)

(155-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

47.807

100

0.5
  comEA/celA/cilE Streptococcus pneumoniae Rx1

46.637

100

0.477
  comEA/celA/cilE Streptococcus pneumoniae D39

46.637

100

0.477
  comEA/celA/cilE Streptococcus pneumoniae R6

46.637

100

0.477
  comEA/celA/cilE Streptococcus mitis NCTC 12261

46.637

100

0.477
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

46.119

100

0.463
  comEA/celA/cilE Streptococcus mitis SK321

44.495

100

0.445
  comEA Lactococcus lactis subsp. cremoris KW2

41.441

100

0.422
  comEA Bacillus subtilis subsp. subtilis str. 168

42.632

87.156

0.372

Multiple sequence alignment