Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SMG00_RS05105 Genome accession   NZ_CP139417
Coordinates   1100017..1101720 (+) Length   567 a.a.
NCBI ID   WP_279647681.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain H46     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1095017..1106720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMG00_RS05080 - 1096004..1096900 (-) 897 WP_279647687.1 ABC transporter ATP-binding protein -
  SMG00_RS05085 - 1096897..1097667 (-) 771 WP_279647686.1 hypothetical protein -
  SMG00_RS05090 - 1097735..1098535 (-) 801 WP_279647685.1 ABC transporter permease -
  SMG00_RS22055 - 1098771..1098914 (-) 144 WP_407834998.1 Tfp structural protein -
  SMG00_RS05095 - 1098999..1099442 (-) 444 WP_279647683.1 hypothetical protein -
  SMG00_RS05100 pilA 1099463..1099648 (-) 186 WP_279647682.1 type IV pilin protein Machinery gene
  SMG00_RS05105 pilB 1100017..1101720 (+) 1704 WP_279647681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SMG00_RS05110 pilC 1101723..1102943 (+) 1221 WP_019405597.1 type II secretion system F family protein Machinery gene
  SMG00_RS05115 pilD 1102947..1103813 (+) 867 WP_320893267.1 A24 family peptidase Machinery gene
  SMG00_RS05120 coaE 1103945..1104553 (+) 609 WP_080940987.1 dephospho-CoA kinase -
  SMG00_RS05125 yacG 1104550..1104747 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  SMG00_RS05130 - 1104760..1105440 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  SMG00_RS05135 - 1105497..1106537 (-) 1041 WP_320893268.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62382.36 Da        Isoelectric Point: 5.4633

>NTDB_id=909431 SMG00_RS05105 WP_279647681.1 1100017..1101720(+) (pilB) [Stutzerimonas stutzeri strain H46]
MNENAPLGGLARQLVLLGQLDEKIAQQAQQQALRNQVPLVTWLVQNKLVKSRVLVDIAGEQFGVPFFDLGTLDKETQPKE
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATSSLEGLNDSELD
GLDIEVAGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQRELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVNVPRDVLLREGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARNDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=909431 SMG00_RS05105 WP_279647681.1 1100017..1101720(+) (pilB) [Stutzerimonas stutzeri strain H46]
ATGAACGAAAACGCGCCCCTTGGCGGCTTGGCCCGGCAGCTGGTTCTCTTAGGCCAGCTAGACGAAAAAATCGCACAGCA
GGCCCAGCAGCAAGCCTTGCGTAACCAAGTGCCTTTGGTGACCTGGTTGGTGCAGAACAAGCTGGTCAAAAGTCGGGTGC
TTGTCGATATCGCCGGTGAGCAGTTTGGTGTTCCGTTCTTTGATTTAGGGACGCTGGACAAAGAAACCCAGCCGAAGGAG
CTGGTCAGCGAGAAGCTGGTGCGCCAGCACCGCGCTCTGCCGTTGCAGCGACGAGGCAACAAGCTTTATGTGGCGGTGTC
CGATCCAACCAACCATCAGGCGATCAGTGATATTCAGTTCAATACGGGGCTTTCCGTTGAGGCTGTGCTGGTTGAGGACG
ACAAGCTCGGTGAGGCCATCGAAAAGTTCTTCGAGTCGGCGACCTCGTCCCTCGAAGGATTGAACGATTCCGAGTTGGAT
GGCTTGGATATCGAAGTGGCCGGTGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCCGATGATGCGCCAGTGGTACG
GTTTGTTAACAAGATGCTGCTCGATGCGATCAAGGGTGGTTCGTCTGATTTGCACTTCGAGCCTTACGAAAAGAGCTATC
GGGTGCGCTTTCGTACGGACGGCATGTTGCACGAGGTTGCTCGCCCGCCGGTCCAGTTGGTCAGCCGTATCTCAGCGCGT
CTGAAAGTAATGGCCAGTCTGGATATCGCTGAGCGGCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGGTCTCGAGCAC
CAAGTCGATCGATTTTCGCGTGAATACGCTACCCACCCTGTGGGGCGAGAAGATTGTTATGCGAATCTTGGATTCTTCCA
GTGCCAAGATGGGTATTGATGCGCTGGGGTATGAGGATGACCAGAGGGAGCTCTATCTAAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGCCCGACAGGCTCGGGCAAGACTGTCTCGCTTTATACCGGCCTGAATATTCTCAATACGGT
CGATGTGAATATCTCCACTGCGGAGGATCCGGTAGAGATCAATTTGGAAGGTATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTCGCCCAGGCGCTTCGAGCGTTCCTGCGCCAGGATCCGGACATCATCATGGTGGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGACACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACTCGCTTGCGCAATATGGGGGTGCCGGCGTTCAACATCGCAACGTCGGTCAATTTGATCATTGCTCAGC
GCCTGGCTCGCAAGCTGTGCCCCAGCTGTAAGCAGGAAGTTAACGTGCCGCGCGATGTTCTGCTCCGAGAGGGATTCCCT
GAGGCGCGAATCGGTTCCTTCAAACTCTATGGGCCAGTCGGGTGCGATAGCTGCAAGGGAGGCTACAAGGGACGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCAGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAACGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTTAAAGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.772

100

0.591
  pilB Acinetobacter baylyi ADP1

58.333

99.471

0.58
  pilB Legionella pneumophila strain ERS1305867

54.895

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

50.912

96.649

0.492
  pilB Vibrio cholerae strain A1552

51.005

96.473

0.492
  pilB Vibrio campbellii strain DS40M4

50.549

96.296

0.487
  pilF Neisseria gonorrhoeae MS11

50.46

95.767

0.483
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.943

93.474

0.383
  pilF Thermus thermophilus HB27

38.188

99.295

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362