Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L6424_RS04230 Genome accession   NZ_AP025277
Coordinates   884569..885678 (-) Length   369 a.a.
NCBI ID   WP_024945839.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain NUITM-VA1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 879569..890678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6424_RS04210 (NUITMVA1_08040) brnQ 880026..881333 (-) 1308 WP_042862965.1 branched-chain amino acid transport system II carrier protein -
  L6424_RS04215 (NUITMVA1_08050) - 881525..882235 (-) 711 WP_024945841.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  L6424_RS04220 (NUITMVA1_08060) srmB 882364..883587 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  L6424_RS04225 (NUITMVA1_08070) yaaA 883764..884537 (-) 774 WP_236766364.1 peroxide stress protein YaaA -
  L6424_RS04230 (NUITMVA1_08080) pilU 884569..885678 (-) 1110 WP_024945839.1 type IVa pilus ATPase TapU Machinery gene
  L6424_RS04235 (NUITMVA1_08090) pilT 885707..886741 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  L6424_RS04240 (NUITMVA1_08100) - 886781..887482 (+) 702 WP_024945838.1 YggS family pyridoxal phosphate-dependent enzyme -
  L6424_RS04245 (NUITMVA1_08110) proC 887541..888365 (+) 825 WP_024945837.1 pyrroline-5-carboxylate reductase -
  L6424_RS04250 (NUITMVA1_08120) - 888406..888957 (+) 552 WP_016351969.1 YggT family protein -
  L6424_RS04255 (NUITMVA1_08130) yggU 888957..889256 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  L6424_RS04260 (NUITMVA1_08140) - 889275..889694 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  L6424_RS04265 (NUITMVA1_08150) - 889749..890060 (-) 312 WP_024945836.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40982.12 Da        Isoelectric Point: 6.3871

>NTDB_id=90937 L6424_RS04230 WP_024945839.1 884569..885678(-) (pilU) [Aeromonas hydrophila strain NUITM-VA1]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFECYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=90937 L6424_RS04230 WP_024945839.1 884569..885678(-) (pilU) [Aeromonas hydrophila strain NUITM-VA1]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGTGCTATCTGCATACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGAGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGTAGCCTGATCACCCAGCGCGAGGTGGGTATAGACACAGCGTCATTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAATCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGCCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36

Multiple sequence alignment