Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SK095_RS19205 Genome accession   NZ_CP139195
Coordinates   4098055..4099755 (-) Length   566 a.a.
NCBI ID   WP_320547184.1    Uniprot ID   -
Organism   Pseudomonas sp. AN-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4093055..4104755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK095_RS19170 (SK095_19170) - 4093116..4093703 (-) 588 WP_201485141.1 DUF4136 domain-containing protein -
  SK095_RS19175 (SK095_19175) - 4093857..4094624 (-) 768 WP_320547179.1 methyltransferase domain-containing protein -
  SK095_RS19180 (SK095_19180) - 4094621..4094926 (-) 306 WP_320548930.1 MazG-like family protein -
  SK095_RS19185 (SK095_19185) yacG 4094932..4095132 (-) 201 WP_320547180.1 DNA gyrase inhibitor YacG -
  SK095_RS19190 (SK095_19190) coaE 4095132..4095740 (-) 609 WP_320547181.1 dephospho-CoA kinase -
  SK095_RS19195 (SK095_19195) pilD 4095938..4096807 (-) 870 WP_320547182.1 A24 family peptidase Machinery gene
  SK095_RS19200 (SK095_19200) pilC 4096811..4098052 (-) 1242 WP_320547183.1 type II secretion system F family protein Machinery gene
  SK095_RS19205 (SK095_19205) pilB 4098055..4099755 (-) 1701 WP_320547184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SK095_RS19210 (SK095_19210) pilA/pilAI 4099984..4100382 (+) 399 WP_320547185.1 pilin Machinery gene
  SK095_RS19215 (SK095_19215) - 4100477..4102300 (+) 1824 WP_320547186.1 hypothetical protein -
  SK095_RS19220 (SK095_19220) - 4102464..4104122 (+) 1659 WP_320547187.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62501.03 Da        Isoelectric Point: 6.0179

>NTDB_id=907825 SK095_RS19205 WP_320547184.1 4098055..4099755(-) (pilB) [Pseudomonas sp. AN-1]
MNDSIALTGLARQLVLAELLDAKTAQQAQAEALRNKIPLVSHLVQNKLVKPRALAEMAAEQFGLPLLELKALDKEAQPRE
LVSEKLIRQHRVLPLLKRGNLLFVGVSDPTNQQAIRDIQFSTGLTVEALLVEDDKLGEAIDKFFDNSTSGMEDLGDVDLD
GVDVEQGGEKKEEAVANETDDSPVVKFVNKMLLDAIRGGSSDLHFEPYEKSYRVRFRTDGILHEVAKPPIQLATRIAARL
KVMAAMDISERRKPQDGRIKLKISANKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEENQKELYLKALSQPQG
MILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDVIMVGEIRDL
ETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARRLCPQCKKIHDIPREALLKEGFPE
EQIGKFKIYQPVGCDACKKGYKGRVGIYEVVKITPALQRIIMEDGNSIEIATKAREEGFNDLRTSGLLKAMQGLTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=907825 SK095_RS19205 WP_320547184.1 4098055..4099755(-) (pilB) [Pseudomonas sp. AN-1]
ATGAACGACTCCATCGCCCTCACCGGTCTGGCCCGCCAACTGGTGCTTGCCGAGCTGCTCGATGCGAAGACTGCCCAGCA
GGCACAGGCCGAAGCGCTGCGCAACAAGATCCCGCTGGTCAGCCATCTGGTACAGAACAAGCTGGTCAAGCCACGTGCAC
TGGCAGAAATGGCCGCCGAGCAGTTCGGCCTGCCGCTGCTGGAGCTGAAGGCGCTGGACAAGGAGGCGCAGCCCAGGGAA
CTGGTCAGCGAGAAACTGATCCGCCAGCACCGCGTGCTGCCGCTGCTCAAGCGCGGCAACCTGCTCTTCGTCGGCGTCTC
CGATCCCACCAACCAGCAGGCGATCCGCGACATCCAGTTCAGCACCGGGCTGACCGTCGAAGCCCTGCTGGTCGAGGACG
ACAAGCTCGGTGAAGCCATCGACAAGTTCTTCGACAACAGCACCAGCGGTATGGAGGATCTCGGCGATGTCGACCTGGAC
GGGGTGGATGTCGAGCAGGGGGGCGAGAAGAAGGAGGAGGCCGTCGCCAACGAAACCGACGACTCCCCGGTGGTCAAGTT
CGTCAACAAGATGCTGCTGGACGCCATTCGCGGCGGCTCCTCCGACCTGCATTTCGAACCCTATGAGAAGAGCTACCGGG
TACGTTTTCGTACCGACGGCATCCTCCACGAGGTGGCCAAACCGCCAATCCAGTTGGCCACCCGCATCGCCGCGCGCCTC
AAGGTGATGGCCGCCATGGACATCTCCGAGCGGCGCAAACCCCAGGACGGACGGATCAAGCTGAAGATCTCCGCCAACAA
GGCCATCGACTTCCGGGTCAACACCCTGCCAACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGTCCAGCG
CCCAGATGGGTATCGATGCCCTCGGCTACGAGGAGAACCAGAAGGAGCTGTACCTGAAGGCGCTCAGCCAGCCGCAGGGC
ATGATCCTGGTAACCGGCCCCACAGGCTCGGGCAAGACGGTTTCCCTATACACCGGCCTGAACATCCTCAACACGCCGGA
CATCAACATTTCCACTGCCGAGGACCCGGTGGAGATCAACCTGGAGGGCATAAACCAGGTCAACGTCAACCCCAAGCAGG
GCATGGACTTCACCCAGGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCGTCAAGGCCGCGCAGACCGGCCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTCACCCGCCTGCGCAACATGGGCGTACCCTCGTTCAACATTGCCACTTCGGTCAACCTGATCATCGCCCAGCGCC
TGGCCCGCCGCCTGTGCCCGCAGTGCAAAAAGATCCACGACATCCCTCGCGAGGCGCTGCTCAAGGAGGGCTTCCCCGAG
GAGCAGATCGGCAAGTTCAAGATCTACCAGCCGGTCGGCTGCGACGCTTGCAAGAAAGGCTACAAGGGCCGCGTGGGTAT
TTATGAAGTGGTTAAAATCACTCCGGCCCTGCAACGGATTATCATGGAGGACGGCAACTCCATCGAAATCGCGACCAAGG
CTCGCGAGGAAGGCTTCAACGACCTGCGCACTTCGGGACTGCTCAAGGCCATGCAAGGCCTGACCAGCCTCGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.092

100

0.613
  pilB Acinetobacter baylyi ADP1

60.32

99.293

0.599
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.551
  pilB Vibrio cholerae strain A1552

50.524

100

0.511
  pilB Vibrio campbellii strain DS40M4

49.735

99.823

0.496
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

52.281

92.933

0.486
  pilB/pilB1 Synechocystis sp. PCC 6803

35.494

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

92.58

0.369
  pilF Thermus thermophilus HB27

39.171

93.816

0.367