Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Cthiooxydans_RS04000 Genome accession   NZ_AP025193
Coordinates   876459..877676 (-) Length   405 a.a.
NCBI ID   WP_003062193.1    Uniprot ID   A0A5S4SYV6
Organism   Comamonas thiooxydans strain NR4028     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 871459..882676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cthiooxydans_RS03970 (Cthiooxydans_07570) - 872268..873203 (+) 936 WP_229228743.1 ATP-binding protein -
  Cthiooxydans_RS03975 (Cthiooxydans_07580) - 873216..873686 (+) 471 WP_052052682.1 NUDIX domain-containing protein -
  Cthiooxydans_RS03980 (Cthiooxydans_07590) - 873804..874013 (-) 210 WP_039049611.1 DNA gyrase inhibitor YacG -
  Cthiooxydans_RS03985 (Cthiooxydans_07600) zapD 874117..874872 (-) 756 WP_039049612.1 cell division protein ZapD -
  Cthiooxydans_RS03990 (Cthiooxydans_07610) coaE 874960..875586 (-) 627 WP_229228151.1 dephospho-CoA kinase -
  Cthiooxydans_RS03995 (Cthiooxydans_07620) - 875590..876459 (-) 870 WP_003062192.1 prepilin peptidase -
  Cthiooxydans_RS04000 (Cthiooxydans_07630) pilC 876459..877676 (-) 1218 WP_003062193.1 type II secretion system F family protein Machinery gene
  Cthiooxydans_RS04005 (Cthiooxydans_07640) pilB 877701..879443 (-) 1743 WP_039049614.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Cthiooxydans_RS04015 (Cthiooxydans_07650) - 879697..880626 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  Cthiooxydans_RS04020 (Cthiooxydans_07660) rplU 880971..881282 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  Cthiooxydans_RS04025 (Cthiooxydans_07670) rpmA 881299..881556 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44513.26 Da        Isoelectric Point: 9.6093

>NTDB_id=90400 Cthiooxydans_RS04000 WP_003062193.1 876459..877676(-) (pilC) [Comamonas thiooxydans strain NR4028]
MASAASKGIKEFLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNVTKLLNDIRSDVETGTSLSAAFRKFPLYFNSLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPTSVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMGISDYFVQYWWLIFGVLGGGIYFFM
QAWKRNERVQQFMDRTILKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYKNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=90400 Cthiooxydans_RS04000 WP_003062193.1 876459..877676(-) (pilC) [Comamonas thiooxydans strain NR4028]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATTTCTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGATCGTTCG
CGGCGAAACTCGTGCCGGCGGAGAAAACCAGATCCAGGCCATGCTGCGCCGCCAGGGCGTGACTCCGTCCAAGATCAAGA
AGCGCAGGACCCGCGGCGGCAAGAAGATCAAGCCCAAGGACATTGCGCTGTTCACGCGCCAGCTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATCGTGGGCCGGGGCAACACCAACCCCAACGTCACCAAGCTGCTCAACGA
CATCCGCTCCGATGTGGAAACCGGCACTTCGCTGAGCGCCGCCTTTCGCAAGTTTCCGCTCTATTTCAACAGCCTCTACT
GCAATCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAGTCGCTGCTGGACCGTCTTGCCACCTATATGGAAAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCCACGTCGGTCATGATCGTGGCCTTTGTCGTGGTGACCGT
GATCATGATCTTCGTGATCCCGGCCTTCAAGGAGGTCTTCACCTCCTTTGGCGCCGACCTGCCCGCGCCCACGCTGCTGG
TGATGGGCATCAGCGATTACTTTGTCCAGTACTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCATCTATTTCTTCATG
CAGGCCTGGAAGCGCAACGAGCGCGTACAGCAGTTCATGGACCGCACCATACTCAAGCTGCCCATCTTCGGCGTGCTGAT
CGAGAAGTCCTGCGTGGCCCGCTGGACACGTACGCTGTCCACCATGTTTGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCCGTGGGAGGCGCCTCGGGCAACTACCTCTACAAAAACGCCACCGACAGGATTCAGTCGGAAGTCTCCACGGGCACC
AGCCTGACCGTGGCCATGGCCAATGCCAATATCTTCCCTTCCATGGTGCTGCAGATGTGTGCCATCGGCGAGGAATCGGG
CGCCATCGACCATATGCTGGGCAAGGCAGCCGATTTCTATGAAAGCGAAGTCGACGAAATGGTGGCGGGCCTCTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
CTGGGTCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4SYV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.465

99.753

0.533
  pilG Neisseria gonorrhoeae MS11

51.889

98.025

0.509
  pilG Neisseria meningitidis 44/76-A

51.637

98.025

0.506
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilC Acinetobacter baylyi ADP1

49.749

98.272

0.489
  pilC Acinetobacter baumannii D1279779

48.363

98.025

0.474
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378
  pilC Thermus thermophilus HB27

36.634

99.753

0.365

Multiple sequence alignment