Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RZE77_RS27080 Genome accession   NZ_CP138513
Coordinates   5766209..5766670 (-) Length   153 a.a.
NCBI ID   WP_319440044.1    Uniprot ID   -
Organism   Variovorax paradoxus strain SPNA7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5749956..5766169 5766209..5766670 flank 40


Gene organization within MGE regions


Location: 5749956..5766670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE77_RS27010 (RZE77_27010) - 5749956..5751119 (-) 1164 WP_319440033.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  RZE77_RS27015 (RZE77_27015) - 5751132..5751710 (-) 579 WP_319440034.1 class I SAM-dependent methyltransferase -
  RZE77_RS27020 (RZE77_27020) - 5751839..5753071 (-) 1233 WP_405546688.1 glycosyltransferase family 4 protein -
  RZE77_RS27025 (RZE77_27025) - 5753175..5754206 (-) 1032 WP_319442422.1 glycosyltransferase -
  RZE77_RS27030 (RZE77_27030) - 5754269..5754958 (-) 690 WP_319440035.1 class I SAM-dependent methyltransferase -
  RZE77_RS27035 (RZE77_27035) - 5754945..5755955 (-) 1011 WP_319440036.1 glycosyltransferase -
  RZE77_RS27040 (RZE77_27040) - 5756300..5756974 (-) 675 WP_319440037.1 class I SAM-dependent methyltransferase -
  RZE77_RS27045 (RZE77_27045) - 5756994..5758385 (-) 1392 WP_319440038.1 lipopolysaccharide biosynthesis protein -
  RZE77_RS27050 (RZE77_27050) - 5758512..5759609 (-) 1098 WP_319442423.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  RZE77_RS27055 (RZE77_27055) - 5759618..5760193 (-) 576 WP_319440039.1 acyltransferase -
  RZE77_RS27060 (RZE77_27060) - 5760190..5761137 (-) 948 WP_319440040.1 Gfo/Idh/MocA family protein -
  RZE77_RS27065 (RZE77_27065) - 5761145..5762449 (-) 1305 WP_319440041.1 nucleotide sugar dehydrogenase -
  RZE77_RS27070 (RZE77_27070) - 5762451..5764379 (-) 1929 WP_319440042.1 polysaccharide biosynthesis protein -
  RZE77_RS27075 (RZE77_27075) - 5764433..5766148 (-) 1716 WP_319440043.1 PglL family O-oligosaccharyltransferase -
  RZE77_RS27080 (RZE77_27080) pilA 5766209..5766670 (-) 462 WP_319440044.1 pilin Machinery gene

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15494.98 Da        Isoelectric Point: 9.9702

>NTDB_id=903276 RZE77_RS27080 WP_319440044.1 5766209..5766670(-) (pilA) [Variovorax paradoxus strain SPNA7]
MNRRLIARNVQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVTEALVIASGAKANIAENAANGASLDAGVEAINAA
SGLKNLSTMSTAAATGTITLVTTAAAKGLTLTLTPTSNGAALQSGTLPPGPVKWTCAVTGAGNNKYVPTECRI

Nucleotide


Download         Length: 462 bp        

>NTDB_id=903276 RZE77_RS27080 WP_319440044.1 5766209..5766670(-) (pilA) [Variovorax paradoxus strain SPNA7]
ATGAACCGTCGTTTGATCGCACGCAACGTGCAAAAGGGTTTCACCCTTATCGAATTGATGATCGTTGTGGCGATCATCGG
TATCTTGGCTGCTGTGGCTCTGCCGGCGTATCAGGATTACACCGTTCGTGCACGCGTCACGGAAGCGCTTGTGATTGCGT
CCGGTGCCAAGGCCAACATTGCCGAAAATGCGGCTAATGGTGCATCGCTCGATGCTGGCGTTGAGGCAATTAACGCCGCT
TCTGGCCTGAAAAATCTCTCGACCATGAGCACCGCGGCTGCGACAGGCACCATCACGCTTGTGACGACTGCTGCAGCAAA
AGGTCTGACTTTGACGCTCACGCCTACGTCGAACGGCGCTGCCTTGCAGAGCGGCACCCTGCCGCCTGGTCCGGTTAAGT
GGACTTGCGCTGTCACCGGAGCTGGGAACAACAAATACGTGCCGACAGAGTGCCGTATTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50

100

0.536
  comP Acinetobacter baylyi ADP1

46.497

100

0.477
  pilA2 Legionella pneumophila strain ERS1305867

51.049

93.464

0.477
  pilA2 Legionella pneumophila str. Paris

51.049

93.464

0.477
  pilA/pilA1 Eikenella corrodens VA1

42.767

100

0.444
  pilE Neisseria gonorrhoeae strain FA1090

38.75

100

0.405
  pilE Neisseria gonorrhoeae MS11

36.747

100

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.667

98.039

0.379
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.095

96.078

0.366