Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   R6238_RS09740 Genome accession   NZ_CP138212
Coordinates   2349044..2350261 (+) Length   405 a.a.
NCBI ID   WP_024960620.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain pss-2019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2344044..2355261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6238_RS09720 - 2344740..2345285 (+) 546 WP_004415097.1 DUF1523 family protein -
  R6238_RS09725 - 2345333..2345920 (-) 588 WP_024960622.1 hypothetical protein -
  R6238_RS09730 - 2346687..2347121 (-) 435 WP_024960621.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  R6238_RS09735 pilB 2347347..2349041 (+) 1695 WP_017278649.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R6238_RS09740 pilC 2349044..2350261 (+) 1218 WP_024960620.1 type II secretion system F family protein Machinery gene
  R6238_RS09745 pilD 2350263..2351135 (+) 873 WP_024960619.1 A24 family peptidase Machinery gene
  R6238_RS09750 coaE 2351132..2351755 (+) 624 WP_004415092.1 dephospho-CoA kinase -
  R6238_RS09755 yacG 2351752..2351961 (+) 210 WP_024960618.1 DNA gyrase inhibitor YacG -
  R6238_RS09760 - 2352088..2352549 (-) 462 WP_024960617.1 hypothetical protein -
  R6238_RS09765 - 2352675..2353304 (+) 630 WP_003406555.1 DUF1780 domain-containing protein -
  R6238_RS09770 - 2353301..2353834 (+) 534 WP_003317783.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43992.04 Da        Isoelectric Point: 10.1848

>NTDB_id=901436 R6238_RS09740 WP_024960620.1 2349044..2350261(+) (pilC) [Pseudomonas syringae pv. syringae strain pss-2019]
MASKAVKVIVYTWEGVDKKGTKASGELSGHNMALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVSSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVAIIVSGILLIKVVPQFQSVFAGFGADLPAFTLMVIGLSNIVQEWWLIIVGLLFAGFFLFK
RAYKKSQKFRDGLDRLLLKAPLIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVIKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFK
LGSIV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=901436 R6238_RS09740 WP_024960620.1 2349044..2350261(+) (pilC) [Pseudomonas syringae pv. syringae strain pss-2019]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACGTGGGAAGGTGTGGACAAGAAGGGCACTAAAGCCAGTGGCGAACT
GAGCGGCCATAATATGGCACTCGTAAAAGCCCAATTGCGCAAACAAGGCATCAACCCCACCAAGGTACGCAAGAAGTCCG
CATCGATCTTCGGCAAAGGCAAGAAGATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTGCCGTTGCTGCAGTCGTTTGACATCATCAGCGAGGGCGCTGAAAACCCAAACATGCGTACACTTGTGAGCTC
GTTAAAGCAGGAAGTGTCGGCAGGCAACAGCTTTGCGACGGCGTTGCGACAGAAACCGGAATATTTCGATGACCTGTTTT
GCAATCTGGTAGATGCCGGCGAGCAAGCGGGGGCACTTGAAAGTCTTCTGGACCGGGTCGCTAGCTACAAGGAGAAGACC
GAAAAGCTAAAAGCCAAAATTAAAAAAGCCATGACATACCCAATCGCCGTGTTGATCGTAGCGATTATTGTGTCAGGTAT
TTTGTTGATCAAGGTGGTACCGCAGTTCCAATCCGTATTTGCTGGCTTTGGTGCTGACTTACCTGCGTTTACTCTGATGG
TTATTGGTCTATCAAACATTGTTCAGGAATGGTGGCTGATAATTGTCGGTTTATTGTTTGCAGGTTTTTTTTTATTTAAG
CGGGCCTACAAAAAATCTCAAAAATTTCGCGACGGCCTTGATCGTCTTTTGCTAAAAGCCCCCCTCATCGGGCCTCTTAT
TTTCAAATCTTCGGTAGCTCGCTATGCGCGCACACTTGCTACGACTTTTGCCGCTGGCGTCCCGCTTGTGGAGGCTTTGG
ACTCAGTCGCCGGAGCGACTGGCAATGTAGTATTTAAAAATGCAGTCATCAAGGTAAAACAGGACGTCTCCACGGGCATG
CAACTGAATTTTTCGATGCGTTCAACAGGAGTATTTCCGAGCCTTGCGATACAGATGACCGCGATTGGCGAAGAGTCCGG
CGCCTTGGACACCATGCTCGACAAGGTCGCTACCTACTATGAGGACGAAGTCGATAACATGGTCGACAATCTGACCAGCC
TTATGGAACCGATGATCATGGCCTTTTTGGGAGTGATCGTCGGCGGCCTTGTCATTGCCATGTACCTCCCCATCTTCAAA
CTTGGAAGCATTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Legionella pneumophila strain ERS1305867

52.217

100

0.523
  pilC Vibrio campbellii strain DS40M4

42.75

98.765

0.422
  pilG Neisseria gonorrhoeae MS11

42.714

98.272

0.42
  pilG Neisseria meningitidis 44/76-A

42.714

98.272

0.42
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Thermus thermophilus HB27

38.404

99.012

0.38