Detailed information NTG0901437

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: R6238_RS09745
• Genome accession: NZ_CP138212
• Coordinates: 2350263..2351135 (+)
• Length: 290 a.a.
• NCBI ID: WP_024960619.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae strain pss-2019
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2345263..2356135

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6238_RS09725	-	2345333..2345920 (-)	588	WP_024960622.1	hypothetical protein	-
R6238_RS09730	-	2346687..2347121 (-)	435	WP_024960621.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
R6238_RS09735	pilB	2347347..2349041 (+)	1695	WP_017278649.1	type IV-A pilus assembly ATPase PilB	Machinery gene
R6238_RS09740	pilC	2349044..2350261 (+)	1218	WP_024960620.1	type II secretion system F family protein	Machinery gene
R6238_RS09745	pilD	2350263..2351135 (+)	873	WP_024960619.1	A24 family peptidase	Machinery gene
R6238_RS09750	coaE	2351132..2351755 (+)	624	WP_004415092.1	dephospho-CoA kinase	-
R6238_RS09755	yacG	2351752..2351961 (+)	210	WP_024960618.1	DNA gyrase inhibitor YacG	-
R6238_RS09760	-	2352088..2352549 (-)	462	WP_024960617.1	hypothetical protein	-
R6238_RS09765	-	2352675..2353304 (+)	630	WP_003406555.1	DUF1780 domain-containing protein	-
R6238_RS09770	-	2353301..2353834 (+)	534	WP_003317783.1	MOSC domain-containing protein	-
R6238_RS09775	-	2354051..2355349 (+)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32114.30 Da
• Isoelectric Point: 8.5809

>NTDB_id=901437 R6238_RS09745 WP_024960619.1 2350263..2351135(+) (pilD) [Pseudomonas syringae pv. syringae strain pss-2019]
MPLLDLLASSPLAFITTCCILGLIIGSFLNVVVYRLPRMMERDWKAQSREMLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSTYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=901437 R6238_RS09745 WP_024960619.1 2350263..2351135(+) (pilD) [Pseudomonas syringae pv. syringae strain pss-2019]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCCTGGCCTTCATCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCGAGAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAAATGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCGCACAAAATCCGC
CCGTGGGAAAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGCAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGACCTATGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCGGCGG
CAATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTTACCTCGCTGAACGATGCGCTGTGGGGTGCAGT
GGCCGGGTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGTTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGCGTGCGTAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.357	97.586	0.521
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.92	94.483	0.5
pilD	Acinetobacter nosocomialis M2	52.555	94.483	0.497
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472