Detailed information NTG0898574

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5015_RS04245
• Genome accession: NZ_CP137505
• Coordinates: 872564..873712 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA0044
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 867564..878712

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5015_RS04225 (R5015_04225)	-	868301..869485 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
R5015_RS04230 (R5015_04230)	-	869482..870855 (-)	1374	WP_126892857.1	pyridoxal-phosphate dependent enzyme	-
R5015_RS04235 (R5015_04235)	-	871098..871502 (-)	405	WP_003084560.1	NINE protein	-
R5015_RS04240 (R5015_04240)	czcD	871657..872556 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
R5015_RS04245 (R5015_04245)	pilU	872564..873712 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5015_RS04250 (R5015_04250)	pilT	873890..874924 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5015_RS04255 (R5015_04255)	-	875139..875831 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5015_RS04260 (R5015_04260)	proC	875843..876664 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5015_RS04265 (R5015_04265)	-	876675..877268 (+)	594	WP_003084544.1	YggT family protein	-
R5015_RS04270 (R5015_04270)	-	877265..877561 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898574 R5015_RS04245 WP_003100959.1 872564..873712(-) (pilU) [Pseudomonas aeruginosa strain HPA0044]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898574 R5015_RS04245 WP_003100959.1 872564..873712(-) (pilU) [Pseudomonas aeruginosa strain HPA0044]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382