Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CYJ98_RS00435 Genome accession   NZ_CP136962
Coordinates   102332..102817 (-) Length   161 a.a.
NCBI ID   WP_101755864.1    Uniprot ID   A0A9X7I5K8
Organism   Neisseria perflava strain UMB0023     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97332..107817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYJ98_RS00420 (CYJ98_000420) - 98606..99796 (+) 1191 WP_101755861.1 efflux RND transporter periplasmic adaptor subunit -
  CYJ98_RS00425 (CYJ98_000425) - 99898..101424 (+) 1527 WP_101755862.1 DHA2 family efflux MFS transporter permease subunit -
  CYJ98_RS00430 (CYJ98_000430) - 101762..102235 (-) 474 WP_101755863.1 pilin -
  CYJ98_RS00435 (CYJ98_000435) pilA2 102332..102817 (-) 486 WP_101755864.1 pilin Machinery gene
  CYJ98_RS00440 (CYJ98_000440) glnD 103608..106166 (-) 2559 WP_101755865.1 [protein-PII] uridylyltransferase -
  CYJ98_RS00445 (CYJ98_000445) - 106186..106482 (-) 297 WP_003680870.1 helix-turn-helix domain-containing protein -
  CYJ98_RS00450 (CYJ98_000450) - 106905..107345 (-) 441 WP_101755866.1 TIGR01244 family sulfur transferase -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16491.03 Da        Isoelectric Point: 7.8157

>NTDB_id=893768 CYJ98_RS00435 WP_101755864.1 102332..102817(-) (pilA2) [Neisseria perflava strain UMB0023]
MKAIQKGFTLIELMIVIAILGILAVIALPAYQDYTIRAKISEGLSLAEPAKLAVAETSAALGGLGQITATNTGYTFPAGG
TKYVSSIAIEAATGKITVTTQKTGASTDPVFTLTPKQANSESPITWECKVTGGEAKHVPANCRPAPTAETPAQPAPAQPA
P

Nucleotide


Download         Length: 486 bp        

>NTDB_id=893768 CYJ98_RS00435 WP_101755864.1 102332..102817(-) (pilA2) [Neisseria perflava strain UMB0023]
ATGAAAGCAATCCAAAAAGGTTTTACCCTGATCGAATTGATGATCGTCATCGCCATCTTGGGCATTTTGGCCGTAATCGC
TCTGCCTGCATACCAAGACTACACCATCCGCGCCAAAATTTCAGAAGGTTTGAGCTTAGCCGAACCTGCTAAATTGGCAG
TAGCAGAAACTTCTGCGGCTTTAGGTGGCTTGGGCCAGATTACTGCTACTAATACTGGTTACACCTTCCCTGCAGGTGGT
ACTAAATACGTTTCAAGCATCGCTATTGAAGCAGCTACCGGTAAAATTACAGTAACAACTCAAAAGACTGGTGCCTCTAC
TGATCCAGTGTTCACATTAACTCCGAAACAAGCTAACTCTGAATCTCCTATTACTTGGGAGTGTAAAGTTACTGGTGGTG
AGGCTAAACACGTTCCAGCTAACTGTCGTCCCGCTCCTACTGCTGAAACCCCGGCTCAACCTGCTCCAGCTCAACCTGCT
CCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

55.944

88.82

0.497
  pilA2 Legionella pneumophila str. Paris

55.944

88.82

0.497
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.508

100

0.429
  pilE Neisseria gonorrhoeae strain FA1090

38.824

100

0.41
  pilA Ralstonia pseudosolanacearum GMI1000

39.521

100

0.41
  comP Acinetobacter baylyi ADP1

40.909

95.652

0.391
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

89.441

0.373
  pilE Neisseria gonorrhoeae MS11

36.81

100

0.373
  pilA Haemophilus influenzae 86-028NP

39.456

91.304

0.36