Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R1T46_RS10430 Genome accession   NZ_CP136693
Coordinates   2254207..2255922 (-) Length   571 a.a.
NCBI ID   WP_317308191.1    Uniprot ID   -
Organism   Marinobacter salarius strain CI.11.F.A3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2249207..2260922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T46_RS10410 (R1T46_10410) tsaA 2250682..2251428 (-) 747 WP_317308187.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  R1T46_RS10415 (R1T46_10415) coaE 2251434..2252036 (-) 603 WP_317308188.1 dephospho-CoA kinase -
  R1T46_RS10420 (R1T46_10420) pilD 2252037..2252912 (-) 876 WP_317308189.1 A24 family peptidase Machinery gene
  R1T46_RS10425 (R1T46_10425) pilC 2252987..2254204 (-) 1218 WP_317308190.1 type II secretion system F family protein Machinery gene
  R1T46_RS10430 (R1T46_10430) pilB 2254207..2255922 (-) 1716 WP_317308191.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R1T46_RS10435 (R1T46_10435) pilA 2256233..2256691 (+) 459 WP_317308192.1 pilin Machinery gene
  R1T46_RS10440 (R1T46_10440) - 2257901..2258329 (+) 429 WP_317308193.1 pilin -
  R1T46_RS10445 (R1T46_10445) - 2258375..2260345 (+) 1971 WP_317308194.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62578.70 Da        Isoelectric Point: 4.8591

>NTDB_id=892491 R1T46_RS10430 WP_317308191.1 2254207..2255922(-) (pilB) [Marinobacter salarius strain CI.11.F.A3]
MATNNSNVTLTGLARRFVEDGLLEEATAKDAFIQASQNKIPLITYLVQNELANSGGLAFSAAMEFGVSVLDLGVFLPDMM
PEKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRAAIDKYLESQDSSMGDLDDA
DLEGVETEGGEQEDDAVVAATDVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKTYRVRYRTDGILKEMSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEEQKKLYMDALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPAEINLEGVNQVNVNNKVGLGFAEALRAFLRQDPDIIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCNSCRQPADVPKDLLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKVTDELANMIMEEASSIKIAKQALAEGFPNLRQSALKKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=892491 R1T46_RS10430 WP_317308191.1 2254207..2255922(-) (pilB) [Marinobacter salarius strain CI.11.F.A3]
ATGGCTACTAACAATTCCAACGTGACGTTGACGGGGCTTGCCCGACGATTTGTGGAAGACGGTCTTCTGGAAGAGGCGAC
GGCTAAAGATGCCTTTATTCAGGCTTCCCAGAACAAGATTCCGCTTATTACCTATCTGGTACAGAACGAACTGGCAAACA
GTGGCGGCCTGGCCTTTTCGGCTGCCATGGAGTTTGGCGTTTCCGTATTGGACCTTGGGGTATTCCTTCCGGACATGATG
CCGGAGAAGGTCGTCGACGAAAAGTTGATTCGCAAACACAATGCACTGCCGCTCTACAAACGGGGAAATCGATTGTTCAT
CGCGGTTTCCGATCCGACCAACATTCAGGCGCTGGACGAGATCAAGTTCAATACGGGCTTGAGCACGGATGCCATTCTGG
TAGATGACGCCAAACTCCGCGCTGCGATAGACAAATATCTTGAATCTCAAGACAGCTCCATGGGTGATCTGGATGATGCC
GATCTTGAAGGCGTGGAAACCGAGGGGGGAGAGCAGGAAGACGACGCTGTTGTCGCTGCCACAGATGTAGACGACGCGCC
TATTGTTAAGTATGTGAACAAAATGCTCCTTGATGCCATTCGTGGCGGTGCGTCCGACGTTCACTTTGAGCCTTACGAGA
AAACCTACCGCGTGCGTTACCGGACTGATGGCATTCTCAAGGAGATGTCCCGCCCCTCCATCAAGCTGGCACCAAAGATA
TCAGCCCGTGTAAAGATCATGGCGCAACTGGATATTTCGGAGCGCCGAGTGCCTCAAGATGGCCGGATCAAAATGAAGCT
GTCGAAAACAAAAGCCATCGACTTCCGAGTGAATACCCTGCCCACTCTATGGGGTGAAAAAATTGTTCTGCGGATTCTCG
ACCCGAGCCAGGCTAAACTGGGCATTGATGTGCTTGGCTATGAAGAAGAGCAGAAGAAGCTGTATATGGACGCCCTGGAA
CAGCCCCAGGGCATGATACTGGTTACGGGCCCGACGGGTTCCGGTAAGACGGTCTCACTCTACACGGGGCTCAACATTCT
AAACACCCCGGAACGGAACATTTCCACCGCAGAAGACCCGGCGGAGATAAACCTGGAGGGTGTTAACCAGGTCAACGTGA
ACAACAAGGTAGGCCTGGGTTTTGCGGAGGCGCTGCGTGCCTTCCTGCGTCAGGATCCGGACATTATCATGGTGGGTGAG
ATTCGGGATCTGGAAACCGCAAATATTGCCATTAAAGCCGCGCAAACCGGCCACTTGGTACTTTCAACCCTTCACACTAA
CAGCGCGGCAGAAACGTTGACCCGTATGATGAATATGGGTGTGCCGGCCTTCAACATTGCCACGTCCGTGAGCCTGATCA
TCGCCCAGCGCCTGGGCCGGCGACTGTGTAACTCTTGCAGACAACCTGCCGACGTCCCCAAGGACCTTCTATTGGCGGAA
GGGTTCACACAAGAACAAATTGATACAGGGTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGAGGATATAA
AGGCCGCGTTGGGATATACGAAGTGGTCAAGGTTACCGACGAACTGGCAAACATGATTATGGAAGAGGCCAGTTCGATTA
AAATTGCAAAGCAAGCACTGGCCGAGGGCTTCCCTAATTTGCGGCAGTCGGCTCTCAAGAAAGTGATTGAGGGTGTGACC
AGCCTTGAGGAAGCCAATCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(18-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.366

100

0.58
  pilB Acinetobacter baylyi ADP1

57.517

100

0.576
  pilB Legionella pneumophila strain ERS1305867

54.087

100

0.545
  pilB Vibrio campbellii strain DS40M4

50.532

98.774

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

50.54

97.373

0.492
  pilB Vibrio cholerae strain A1552

53.846

91.068

0.49
  pilF Neisseria gonorrhoeae MS11

47.08

98.949

0.466
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.304

92.119

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

90.893

0.368
  pilF Thermus thermophilus HB27

37.726

97.023

0.366