Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OCV27_RS01260 Genome accession   NZ_AP024889
Coordinates   270149..271501 (+) Length   450 a.a.
NCBI ID   WP_245986609.1    Uniprot ID   -
Organism   Vibrio pectenicida strain LMG 19642     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 265149..276501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV27_RS01240 pilM 267614..268537 (+) 924 WP_185759717.1 pilus assembly protein PilM -
  OCV27_RS01245 - 268527..269084 (+) 558 WP_125319349.1 PilN domain-containing protein -
  OCV27_RS01250 pilO 269077..269664 (+) 588 WP_185759718.1 type 4a pilus biogenesis protein PilO -
  OCV27_RS01255 - 269654..270127 (+) 474 WP_125319351.1 pilus assembly protein PilP -
  OCV27_RS01260 pilQ 270149..271501 (+) 1353 WP_245986609.1 type IV pilus secretin PilQ Machinery gene
  OCV27_RS01265 aroK 271735..272253 (+) 519 WP_125319353.1 shikimate kinase AroK -
  OCV27_RS01270 aroB 272285..273373 (+) 1089 WP_125319354.1 3-dehydroquinate synthase -
  OCV27_RS01275 - 273416..274903 (+) 1488 WP_125319355.1 SPOR domain-containing protein -
  OCV27_RS01280 - 274974..275801 (+) 828 WP_125319356.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 49172.78 Da        Isoelectric Point: 4.8309

>NTDB_id=89164 OCV27_RS01260 WP_245986609.1 270149..271501(+) (pilQ) [Vibrio pectenicida strain LMG 19642]
MIKKILMLISIYLFSITYVCAETIVSFQSDTSEDERLGRKLSVNFQDIPVRSVLQQLAEYSDFNLVVSDSVSGKVTLRLK
EVYWSHLLDMILLIKGLEKRRIGDSLLVLPKSELGMLNDKLSPKLESLESVIIAMRYANATDIADILGNEATTSMLSEFG
SISVDERTNALLIRDHMTYIRAMKPIIAALDVPVKQVQIEARIVSLSEGDVQELGVRWGLSAMKGGYSLGGSIENNLSNI
SLYTESEATLDDFLNVDLAVTSSTASTIAFQVAKLSSGILLDLELSALQREARAEVISSPRLITTNKQAAYIEQGTEIPY
LEAASSGATSVNFKKAVLSLKVTPQITADNRLILDLKVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLINDGETIVLGGIY
QHSVSDAVEKVPLLGDIPIIGGLFRRSYQQMGKSELLIFVTPTVIFPRAI

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=89164 OCV27_RS01260 WP_245986609.1 270149..271501(+) (pilQ) [Vibrio pectenicida strain LMG 19642]
GTGATTAAAAAAATCCTGATGCTGATAAGTATTTATTTGTTTTCAATTACATATGTTTGTGCTGAAACCATCGTTTCCTT
TCAATCTGACACATCAGAAGATGAACGCCTTGGTCGAAAATTATCTGTCAATTTTCAAGATATTCCGGTTCGTAGTGTAT
TGCAACAGCTTGCGGAATACAGTGATTTTAATTTAGTTGTATCGGATTCTGTATCAGGAAAGGTGACTCTTAGGCTTAAA
GAGGTTTATTGGTCGCACTTACTTGATATGATTTTATTGATTAAAGGACTTGAAAAGCGTCGAATTGGCGATAGTTTGTT
AGTCTTGCCAAAATCTGAATTAGGGATGCTTAATGACAAGCTTTCTCCAAAATTGGAATCACTTGAATCAGTCATTATTG
CCATGCGCTATGCTAATGCAACGGATATCGCTGATATACTTGGCAATGAAGCAACAACCTCCATGCTGTCTGAATTTGGT
TCTATATCGGTAGATGAAAGAACCAATGCTCTATTGATTCGAGATCACATGACGTATATTCGAGCGATGAAGCCTATCAT
AGCGGCGTTAGATGTACCGGTTAAGCAAGTACAGATTGAGGCGCGTATAGTTAGTTTAAGCGAGGGAGATGTACAAGAAC
TTGGTGTTCGCTGGGGGCTAAGCGCGATGAAAGGTGGTTATTCTCTTGGGGGGTCAATAGAAAATAACCTCTCCAATATT
AGTTTATATACGGAAAGTGAAGCGACTCTTGATGATTTTCTTAATGTGGATCTCGCTGTGACTTCTAGTACTGCCTCGAC
AATTGCCTTTCAAGTCGCTAAATTGAGTAGTGGGATCTTGCTTGACCTTGAGTTATCAGCGTTGCAAAGAGAAGCTCGTG
CTGAGGTAATATCTAGTCCTCGTTTGATTACAACCAATAAGCAAGCTGCCTATATTGAACAAGGTACGGAAATTCCATAT
TTGGAAGCCGCATCAAGTGGAGCGACGAGTGTCAATTTTAAAAAGGCGGTTTTAAGCCTTAAAGTAACGCCACAAATTAC
TGCTGATAACCGCTTAATTTTAGATTTAAAGGTTACTCAGGACAGGCCTGGCCAAGTTGTGAAAACGGGTACAGGTGAAG
CGGTGGCTATAGATACCCAGCGGATAGGTACTCAGGTGCTTATTAATGATGGTGAAACAATAGTATTAGGAGGGATTTAT
CAACACAGTGTGTCAGATGCTGTTGAAAAAGTGCCACTTTTGGGAGATATTCCAATAATAGGGGGCCTATTTAGGCGGAG
CTATCAGCAAATGGGCAAGAGTGAATTGCTTATTTTTGTTACACCAACTGTGATTTTCCCTAGGGCAATATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(287-445)

Bacterial type II/III secretion system short domain (Secretin_N)

(132-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

63.146

94.667

0.598
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

63.876

92.889

0.593
  pilQ Vibrio cholerae strain A1552

63.876

92.889

0.593
  pilQ Legionella pneumophila strain ERS1305867

46.301

93.111

0.431
  pilQ Legionella pneumophila strain Lp02

46.301

93.111

0.431
  pilQ Pseudomonas aeruginosa PAK

40.821

100

0.42
  comQ Acinetobacter baylyi ADP1

40.807

99.111

0.404
  comE Haemophilus influenzae Rd KW20

40.089

99.778

0.4
  pilQ Acinetobacter baumannii strain A118

38.696

100

0.396
  pilQ Acinetobacter baumannii D1279779

38.696

100

0.396
  comE Haemophilus influenzae 86-028NP

39.286

99.556

0.391

Multiple sequence alignment