Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   RX142_RS02345 Genome accession   NZ_CP136341
Coordinates   458792..459250 (+) Length   152 a.a.
NCBI ID   WP_002365911.1    Uniprot ID   Q8VT46
Organism   Enterococcus faecalis strain ES-397     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 433842..492507 458792..459250 within 0


Gene organization within MGE regions


Location: 433842..492507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RX142_RS02195 (RX142_02195) - 436774..437613 (-) 840 WP_049142639.1 LysM domain-containing protein -
  RX142_RS02200 (RX142_02200) - 437758..438003 (+) 246 WP_049142638.1 hypothetical protein -
  RX142_RS02205 (RX142_02205) - 438066..438488 (+) 423 WP_049142637.1 hypothetical protein -
  RX142_RS02210 (RX142_02210) - 438582..438899 (-) 318 WP_049142636.1 hypothetical protein -
  RX142_RS02215 (RX142_02215) - 438926..439132 (-) 207 WP_049142635.1 hypothetical protein -
  RX142_RS02220 (RX142_02220) - 439113..439295 (-) 183 WP_049142634.1 hypothetical protein -
  RX142_RS02225 (RX142_02225) - 439309..439659 (-) 351 WP_049142633.1 hypothetical protein -
  RX142_RS02230 (RX142_02230) - 439685..439987 (-) 303 WP_049142632.1 hypothetical protein -
  RX142_RS02235 (RX142_02235) - 439990..440223 (-) 234 WP_049142631.1 hypothetical protein -
  RX142_RS02240 (RX142_02240) - 441127..441657 (-) 531 WP_228394825.1 hypothetical protein -
  RX142_RS02245 (RX142_02245) - 441654..442769 (-) 1116 WP_049142629.1 replication initiator protein A -
  RX142_RS02250 (RX142_02250) - 442775..443173 (-) 399 WP_049142628.1 replication initiator protein A -
  RX142_RS02255 (RX142_02255) - 443868..444088 (+) 221 Protein_422 hypothetical protein -
  RX142_RS02260 (RX142_02260) - 444413..444658 (-) 246 WP_002358448.1 helix-turn-helix domain-containing protein -
  RX142_RS02265 (RX142_02265) - 444652..445053 (-) 402 WP_002358449.1 RNA polymerase sigma factor -
  RX142_RS02270 (RX142_02270) - 445611..445966 (+) 356 Protein_425 sigma-70 family RNA polymerase sigma factor -
  RX142_RS02275 (RX142_02275) - 447050..450955 (+) 3906 WP_396336035.1 LPXTG-anchored aggregation substance -
  RX142_RS02280 (RX142_02280) prgU 451017..451370 (+) 354 WP_002379922.1 pheromone response system RNA-binding regulator PrgU -
  RX142_RS02285 (RX142_02285) - 451392..451778 (+) 387 WP_002389861.1 hypothetical protein -
  RX142_RS02290 (RX142_02290) - 451847..452107 (+) 261 WP_002370667.1 hypothetical protein -
  RX142_RS02295 (RX142_02295) - 452118..452996 (+) 879 WP_316498890.1 hypothetical protein -
  RX142_RS02300 (RX142_02300) - 453016..453876 (+) 861 WP_010714270.1 LPXTG cell wall anchor domain-containing protein -
  RX142_RS02305 (RX142_02305) - 453902..455173 (+) 1272 WP_002409353.1 CHAP domain-containing protein -
  RX142_RS02310 (RX142_02310) - 455176..455793 (+) 618 WP_002409352.1 hypothetical protein -
  RX142_RS02315 (RX142_02315) - 455777..456193 (+) 417 WP_002406548.1 thioredoxin domain-containing protein -
  RX142_RS02320 (RX142_02320) - 456193..456507 (+) 315 WP_002360796.1 hypothetical protein -
  RX142_RS02325 (RX142_02325) - 456500..457534 (+) 1035 WP_002385553.1 conjugal transfer protein -
  RX142_RS02330 (RX142_02330) - 457539..457799 (+) 261 WP_010706916.1 hypothetical protein -
  RX142_RS02335 (RX142_02335) - 457799..458188 (+) 390 WP_002360791.1 TcpE family conjugal transfer membrane protein -
  RX142_RS02340 (RX142_02340) - 458220..458702 (+) 483 WP_002360790.1 hypothetical protein -
  RX142_RS02345 (RX142_02345) ssb 458792..459250 (+) 459 WP_002365911.1 single-stranded DNA-binding protein Machinery gene
  RX142_RS02350 (RX142_02350) - 459355..461847 (+) 2493 WP_002377939.1 ATP-binding protein -
  RX142_RS02355 (RX142_02355) - 461804..462202 (+) 399 WP_002370287.1 lipocalin-like domain-containing protein -
  RX142_RS02360 (RX142_02360) - 462215..464560 (+) 2346 WP_002377940.1 CD3337/EF1877 family mobilome membrane protein -
  RX142_RS02365 (RX142_02365) - 464547..466805 (+) 2259 WP_316498891.1 TraM recognition domain-containing protein -
  RX142_RS02370 (RX142_02370) - 467328..468113 (+) 786 WP_002360778.1 replication-relaxation family protein -
  RX142_RS02375 (RX142_02375) - 468119..468607 (+) 489 WP_010710133.1 hypothetical protein -
  RX142_RS02380 (RX142_02380) - 469199..469465 (+) 267 WP_002377947.1 hypothetical protein -
  RX142_RS02385 (RX142_02385) - 469498..469998 (+) 501 WP_002360775.1 DnaJ domain-containing protein -
  RX142_RS02390 (RX142_02390) - 470011..470259 (+) 249 WP_002360773.1 DUF3850 domain-containing protein -
  RX142_RS02395 (RX142_02395) - 470335..470751 (+) 417 WP_010710134.1 single-stranded DNA-binding protein -
  RX142_RS02400 (RX142_02400) - 470775..471359 (+) 585 WP_002377949.1 thermonuclease family protein -
  RX142_RS02405 (RX142_02405) - 471470..471736 (+) 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  RX142_RS02410 (RX142_02410) - 471729..472004 (+) 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin -
  RX142_RS02415 (RX142_02415) - 472195..472839 (-) 645 WP_002289351.1 IS6 family transposase -
  RX142_RS02420 (RX142_02420) bsh 473063..474037 (+) 975 WP_002355428.1 choloylglycine hydrolase -
  RX142_RS02425 (RX142_02425) - 474706..475314 (-) 609 Protein_456 IS6 family transposase -
  RX142_RS02430 (RX142_02430) - 475346..475531 (-) 186 WP_002358660.1 hypothetical protein -
  RX142_RS02435 (RX142_02435) - 475621..475890 (+) 270 Protein_458 LPXTG cell wall anchor domain-containing protein -
  RX142_RS02440 (RX142_02440) - 476018..476956 (+) 939 WP_002370935.1 2-dehydropantoate 2-reductase -
  RX142_RS02445 (RX142_02445) - 476982..478019 (+) 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
  RX142_RS02450 (RX142_02450) - 478324..479209 (-) 886 Protein_461 IS256 family transposase -
  RX142_RS02455 (RX142_02455) - 479339..479983 (+) 645 WP_002289351.1 IS6 family transposase -
  RX142_RS02460 (RX142_02460) - 480156..480470 (+) 315 WP_002403043.1 hypothetical protein -
  RX142_RS02465 (RX142_02465) cylR2 480808..481008 (-) 201 WP_002370931.1 cytolysin regulator CylR2 -
  RX142_RS02470 (RX142_02470) cylL-L 481412..481618 (+) 207 WP_002358485.1 lanthipeptide cytolysin subunit CylL-L -
  RX142_RS02475 (RX142_02475) cylL-S 481652..481843 (+) 192 WP_002358181.1 lanthipeptide cytolysin subunit CylL-S -
  RX142_RS02480 (RX142_02480) - 481904..484885 (+) 2982 WP_002370929.1 type 2 lanthipeptide synthetase LanM family protein -
  RX142_RS02485 (RX142_02485) - 484897..485403 (+) 507 WP_316498892.1 cysteine peptidase family C39 domain-containing protein -
  RX142_RS02490 (RX142_02490) - 485419..487041 (+) 1623 WP_263711767.1 peptidase domain-containing ABC transporter -
  RX142_RS02495 (RX142_02495) - 487038..488276 (+) 1239 WP_002368283.1 S8 family serine peptidase -
  RX142_RS02500 (RX142_02500) - 488277..489260 (+) 984 WP_002368282.1 site-2 protease family protein -
  RX142_RS02505 (RX142_02505) - 489613..490176 (+) 564 WP_002370925.1 hypothetical protein -
  RX142_RS02510 (RX142_02510) - 490613..491080 (+) 468 WP_002370921.1 hypothetical protein -
  RX142_RS02515 (RX142_02515) - 491158..491739 (+) 582 WP_002401490.1 hypothetical protein -
  RX142_RS02520 (RX142_02520) - 491834..492040 (+) 207 WP_229017897.1 S41 family peptidase -

Sequence


Protein


Download         Length: 152 a.a.        Molecular weight: 17179.08 Da        Isoelectric Point: 4.6511

>NTDB_id=890117 RX142_RS02345 WP_002365911.1 458792..459250(+) (ssb) [Enterococcus faecalis strain ES-397]
MINNVTLVGRLTKDPDLRYTQSGTAVGQFTLAINRNFTNANNEREADFINCVIWRKAAESLANYATKGTLIGLTGRIQTR
NYENQQGQRIYVTEVVTESFQLLESREVNEQRKEQATGKATFDKQSMDKPDPLDPFSPENSIVDISDNDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=890117 RX142_RS02345 WP_002365911.1 458792..459250(+) (ssb) [Enterococcus faecalis strain ES-397]
TTGATTAATAACGTTACATTAGTTGGACGATTAACCAAAGACCCAGATTTAAGGTATACGCAAAGTGGAACAGCCGTAGG
TCAATTTACGTTGGCCATTAATCGCAACTTTACCAATGCTAACAATGAAAGGGAAGCAGATTTTATCAACTGTGTTATTT
GGCGGAAAGCTGCAGAGTCATTAGCAAATTATGCAACAAAAGGGACTCTGATCGGTTTAACTGGTCGCATTCAAACAAGA
AACTATGAGAATCAACAAGGCCAGCGTATTTATGTAACTGAGGTTGTCACAGAAAGCTTCCAACTATTAGAATCAAGAGA
AGTAAACGAGCAACGAAAAGAACAGGCTACAGGTAAAGCTACGTTTGATAAACAGTCAATGGATAAACCTGATCCTCTGG
ATCCATTTTCGCCAGAAAATAGCATAGTGGATATTTCTGATAATGACCTGCCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB Q8VT46

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.632

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.283

100

0.572

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

69.737

0.395

  ssbB/cilA Streptococcus mitis NCTC 12261

50

76.316

0.382

  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.774

69.737

0.375

  ssbB/cilA Streptococcus pneumoniae TIGR4

49.138

76.316

0.375

  ssbB/cilA Streptococcus pneumoniae Rx1

48.276

76.316

0.368

  ssbB/cilA Streptococcus pneumoniae D39

48.276

76.316

0.368

  ssbB/cilA Streptococcus pneumoniae R6

48.276

76.316

0.368

  ssbB/cilA Streptococcus mitis SK321

48.276

76.316

0.368