Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RS399_RS19120 Genome accession   NZ_CP135973
Coordinates   3789180..3789797 (+) Length   205 a.a.
NCBI ID   WP_084993651.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain SI3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3784180..3794797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS399_RS19085 (RS399_19085) aroE 3784838..3785680 (+) 843 WP_168767822.1 shikimate dehydrogenase -
  RS399_RS19090 (RS399_19090) yhbY 3785674..3785964 (+) 291 WP_084993643.1 ribosome assembly RNA-binding protein YhbY -
  RS399_RS19095 (RS399_19095) - 3785976..3786545 (+) 570 WP_019258940.1 nicotinate-nucleotide adenylyltransferase -
  RS399_RS19100 (RS399_19100) yqeK 3786535..3787095 (+) 561 WP_084993645.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RS399_RS19105 (RS399_19105) rsfS 3787113..3787469 (+) 357 WP_003237112.1 ribosome silencing factor -
  RS399_RS19110 (RS399_19110) - 3787466..3788209 (+) 744 WP_084993647.1 class I SAM-dependent methyltransferase -
  RS399_RS19115 (RS399_19115) comER 3788275..3789096 (-) 822 WP_084993649.1 late competence protein ComER -
  RS399_RS19120 (RS399_19120) comEA 3789180..3789797 (+) 618 WP_084993651.1 helix-hairpin-helix domain-containing protein Machinery gene
  RS399_RS19125 (RS399_19125) - 3789864..3790433 (+) 570 WP_003237106.1 ComE operon protein 2 -
  RS399_RS19130 (RS399_19130) comEC 3790437..3792767 (+) 2331 WP_084993894.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RS399_RS19135 (RS399_19135) - 3792807..3792941 (-) 135 WP_003229983.1 YqzM family protein -
  RS399_RS19140 (RS399_19140) - 3792982..3793131 (+) 150 WP_142271151.1 hypothetical protein -
  RS399_RS19145 (RS399_19145) holA 3793171..3794214 (+) 1044 WP_084993653.1 DNA polymerase III subunit delta -
  RS399_RS19150 (RS399_19150) rpsT 3794229..3794495 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21876.65 Da        Isoelectric Point: 4.7416

>NTDB_id=888045 RS399_RS19120 WP_084993651.1 3789180..3789797(+) (comEA) [Bacillus inaquosorum strain SI3]
MNWLNQHKKTIILAASAAVFIALFFIFSSGKNKEPVKQAVSTETENAEVKQEAVKDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGASEQADEMQVNLAELLQDGTVVYIPKKGEEPAVQQGAGGAVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=888045 RS399_RS19120 WP_084993651.1 3789180..3789797(+) (comEA) [Bacillus inaquosorum strain SI3]
ATGAATTGGTTGAATCAGCATAAAAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGTTATTTTTCATCTT
TTCCAGCGGAAAAAACAAAGAGCCGGTGAAGCAGGCTGTATCAACTGAGACAGAAAATGCGGAGGTAAAGCAGGAAGCAG
TAAAAGACGAGTCAAACGAAACAATTGTGATTGACATTAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGAGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGGCCAGTGAACAAGCAGACGAAATGCAAGTGAACTTGGCGGA
GCTGCTTCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAGCCGGCGGTGCAGCAAGGAGCCGGAGGCGCTG
TTCAAAGCGATGGAGGGAAGGGAGCGCTCGTAAATATCAATACAGCCACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGACCGTCAAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGGCGTTTCCAGACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

90.244

100

0.902
  comEA/celA/cilE Streptococcus mitis NCTC 12261

39.806

100

0.4
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

37.736

100

0.39
  comEA/celA/cilE Streptococcus mitis SK321

38.835

100

0.39
  comEA/celA/cilE Streptococcus pneumoniae D39

38.164

100

0.385
  comEA/celA/cilE Streptococcus pneumoniae Rx1

38.164

100

0.385
  comEA/celA/cilE Streptococcus pneumoniae R6

38.164

100

0.385
  comEA Staphylococcus aureus MW2

36.967

100

0.38
  comEA Staphylococcus aureus N315

35.545

100

0.366
  comEA Latilactobacillus sakei subsp. sakei 23K

35.407

100

0.361