Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OCU77_RS01150 Genome accession   NZ_AP024852
Coordinates   250437..252140 (+) Length   567 a.a.
NCBI ID   WP_107302833.1    Uniprot ID   A0A2T3P5Q4
Organism   Photobacterium swingsii strain CECT 7576     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 245437..257140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU77_RS01130 pilM 247652..248668 (+) 1017 WP_107302832.1 type IV pilus biogenesis protein PilM -
  OCU77_RS01135 - 248665..249270 (+) 606 WP_048898883.1 PilN domain-containing protein -
  OCU77_RS01140 - 249267..249881 (+) 615 WP_048898884.1 type 4a pilus biogenesis protein PilO -
  OCU77_RS01145 - 249881..250414 (+) 534 WP_048898885.1 pilus assembly protein PilP -
  OCU77_RS01150 pilQ 250437..252140 (+) 1704 WP_107302833.1 type IV pilus secretin PilQ Machinery gene
  OCU77_RS01155 aroK 252304..252822 (+) 519 WP_048898886.1 shikimate kinase AroK -
  OCU77_RS01160 aroB 252844..253923 (+) 1080 WP_048898887.1 3-dehydroquinate synthase -
  OCU77_RS01165 - 253971..255476 (+) 1506 WP_239685964.1 SPOR domain-containing protein -
  OCU77_RS01170 - 255585..256403 (+) 819 WP_048898889.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  OCU77_RS01175 - 256422..256631 (-) 210 WP_048898890.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61959.00 Da        Isoelectric Point: 4.6573

>NTDB_id=88792 OCU77_RS01150 WP_107302833.1 250437..252140(+) (pilQ) [Photobacterium swingsii strain CECT 7576]
MFTKLAADKRFSALLYGIAWLVLLLMFPRWAYAGNDIAALDFHRSEKGQGVLSVQLEKAQSLIDIRQSGLQLYVEVVDGT
IADEMLYVLDVNDFATAVKTVETFRSDKGVKLLLTMSERFDYDYKTSGNQLDVIVTAKPKSTETDEQNITYNGKPISINF
QDVPVRNVLQLIADYNDFNLVVSDTVEGNVTLRLDDVPWPQVLDIILQSKGLDKRINGKVVLVAPKSELDGHEQQALAAK
RKSEELAALRSELIQVKYAKAIDLASLLIGDEDGVSMLSARGSLHVDERTNSLIIKDLPESIESVKDILSALDIPVKQVQ
IEARIVTVSEGDLEELGVRWGVISNNGDTTIGGSVESNLHNSGLLDRKATVDDFLNVNLGATTAGASSIAFQVAKLGDIL
LDLELSALQKEEKAEIISSPRLVTTNKKTAYIEQGTEIPYLEGSSSGAVTVSFKKAVLSLMVTPQITPDNKLVLDLVVTQ
DKVGKTVKTGTGEAVAIDTQRIGTQVLVNNGETLVLGGIYQHALSTKVEQVPLLGDIPLLGALFRRTKEDLDKRELLIFV
TPKIVFQ

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=88792 OCU77_RS01150 WP_107302833.1 250437..252140(+) (pilQ) [Photobacterium swingsii strain CECT 7576]
ATGTTTACAAAGTTAGCGGCCGATAAGCGCTTTTCAGCCTTGTTGTATGGGATCGCTTGGCTCGTGTTGCTATTGATGTT
TCCTCGCTGGGCGTATGCAGGCAATGATATTGCAGCACTCGATTTTCATCGAAGTGAAAAGGGGCAAGGGGTATTGAGTG
TTCAACTTGAAAAAGCCCAATCCTTGATCGATATTCGGCAGTCAGGTCTGCAGCTGTACGTGGAAGTTGTTGATGGCACG
ATTGCTGATGAAATGCTGTATGTGCTTGATGTGAATGATTTTGCTACCGCAGTAAAAACAGTTGAAACCTTTCGCAGCGA
TAAAGGCGTTAAGCTGTTACTGACGATGTCAGAGCGCTTTGATTACGATTATAAAACCAGTGGTAATCAACTGGATGTCA
TTGTGACAGCCAAGCCCAAATCCACGGAAACTGATGAGCAGAACATAACCTATAACGGCAAACCTATCTCAATTAACTTT
CAAGATGTCCCTGTACGCAATGTTTTGCAGCTGATTGCCGATTATAACGATTTTAATTTGGTGGTATCGGATACGGTGGA
AGGCAATGTGACTCTGCGCCTCGATGATGTGCCATGGCCACAAGTGCTCGATATTATTTTACAGTCAAAAGGGCTCGATA
AGCGTATTAACGGCAAGGTGGTGTTGGTGGCGCCTAAATCGGAGCTGGACGGGCATGAACAACAAGCCTTGGCTGCAAAG
CGTAAATCGGAAGAACTCGCGGCATTGCGCTCTGAATTGATCCAGGTGAAATATGCCAAAGCAATTGATTTAGCGAGTTT
GTTAATCGGGGATGAAGATGGTGTCAGTATGCTGTCTGCACGTGGCTCGCTTCATGTCGATGAACGTACTAATTCACTGA
TTATCAAAGATCTGCCTGAGAGCATTGAAAGTGTGAAAGATATTTTGAGTGCGCTAGATATTCCGGTTAAGCAGGTGCAG
ATTGAGGCGCGTATTGTGACCGTGAGTGAAGGTGACTTAGAGGAGCTTGGGGTTCGCTGGGGGGTGATCAGTAATAACGG
TGATACCACGATAGGTGGTTCAGTCGAAAGCAATTTACATAATTCTGGATTGCTGGATAGAAAAGCCACTGTTGATGATT
TTTTGAATGTCAATCTAGGGGCAACGACTGCAGGGGCATCAAGTATTGCATTTCAAGTGGCAAAACTGGGCGATATATTG
CTAGATTTAGAACTGTCTGCGCTGCAGAAGGAAGAAAAAGCGGAGATTATCTCAAGCCCTCGTTTAGTGACGACAAATAA
GAAAACAGCCTATATCGAGCAAGGGACCGAGATCCCATACCTTGAAGGTTCATCCAGTGGTGCGGTAACGGTTAGCTTCA
AAAAAGCGGTTTTGAGCTTAATGGTGACGCCGCAAATCACACCGGATAATAAATTGGTGCTGGACTTAGTGGTCACGCAA
GATAAAGTCGGTAAAACGGTTAAAACGGGAACGGGTGAGGCTGTTGCCATTGATACCCAGCGAATTGGCACCCAAGTGCT
CGTCAATAATGGTGAAACCTTGGTTTTGGGGGGGATTTATCAACATGCGTTGTCAACAAAAGTCGAACAAGTGCCACTTT
TAGGGGATATTCCTTTGTTAGGGGCGCTATTTCGTCGTACCAAGGAAGACTTAGACAAGCGTGAGCTGTTGATTTTTGTC
ACTCCGAAGATTGTTTTTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(252-318)

Secretin and TonB N terminus short domain (STN)

(178-226)

Bacterial type II and III secretion system protein (Secretin)

(407-565)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3P5Q4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.101

99.295

0.607
  pilQ Vibrio cholerae strain A1552

61.101

99.295

0.607
  pilQ Vibrio campbellii strain DS40M4

60.917

96.12

0.586
  pilQ Pseudomonas aeruginosa PAK

42.143

98.765

0.416
  pilQ Legionella pneumophila strain ERS1305867

42.357

95.767

0.406
  pilQ Legionella pneumophila strain Lp02

42.357

95.767

0.406
  comQ Acinetobacter baylyi ADP1

40.142

99.295

0.399
  pilQ Acinetobacter baumannii D1279779

38.754

100

0.395
  pilQ Acinetobacter baumannii strain A118

38.754

100

0.395

Multiple sequence alignment