Detailed information NTG0887413

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: RS163_RS01990
• Genome accession: NZ_CP135688
• Coordinates: 429421..430554 (-)
• Length: 377 a.a.
• NCBI ID: WP_097756639.1
• Uniprot ID: -
• Organism: Escherichia coli strain TUM2820
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	430551..430928	429421..430554	flank	-3

Gene organization within MGE regions

Location: 429421..430928

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RS163_RS01990 (RS163_01990)	pilB	429421..430554 (-)	1134	WP_097756639.1	GspE/PulE family protein	Machinery gene

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41720.15 Da
• Isoelectric Point: 9.0920

>NTDB_id=887413 RS163_RS01990 WP_097756639.1 429421..430554(-) (pilB) [Escherichia coli strain TUM2820]
MSWSHYPASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALLISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRV
STLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLR
DMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVT
PELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA

Nucleotide

• Length: 1134 bp

>NTDB_id=887413 RS163_RS01990 WP_097756639.1 429421..430554(-) (pilB) [Escherichia coli strain TUM2820]
ATAAGTTGGAGTCATTACCCCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCCGTTTTCGCATCGA
CGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTC
TTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTA
TCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAA
CCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTTCCGCACGGTATTATCCTGGTGACAGGGC
CGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAATACTCCCGGCCGCAATATTCTGACGGTA
GAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGG
CCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGG
TTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCCGC
GACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCGCGCAACGTCTGGTTCGTCGCCTGTGTCC
GCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAATATCATCAGCTCGCGGTGACAACAATTG
GCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCATGGCGATCCACGAAATGATGGTGGTGACG
CCGGAATTACGGGCCGCTATTCATGAAAATGTCGATGAACAAGCACTGGAGCGACTAGTCCGGCAACAACACAAGGCCTT
AATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAGGTTATGCGCGTCGCCAGTGCCACGCTGG
AGAGCGAAGCATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (6-264)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	49.33	98.939	0.488
pilB	Vibrio cholerae strain A1552	50.549	96.552	0.488
pilB	Vibrio campbellii strain DS40M4	50.549	96.552	0.488
pilB	Acinetobacter baylyi ADP1	48.794	98.939	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	48.913	97.613	0.477
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	46.467	97.613	0.454
pilB/pilB1	Synechocystis sp. PCC 6803	43.702	100	0.451
pilF	Thermus thermophilus HB27	46.961	96.021	0.451
pilF	Neisseria gonorrhoeae MS11	45.753	96.817	0.443
pilB	Legionella pneumophila strain ERS1305867	45.082	97.082	0.438
ctsE	Campylobacter jejuni subsp. jejuni 81-176	43.443	97.082	0.422
pilB	Haemophilus influenzae Rd KW20	42.818	97.878	0.419
pilB	Haemophilus influenzae 86-028NP	43.094	96.021	0.414
pilB	Glaesserella parasuis strain SC1401	47.267	82.493	0.39