Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RRV97_RS07845 Genome accession   NZ_CP135440
Coordinates   1682386..1683627 (+) Length   413 a.a.
NCBI ID   WP_315611201.1    Uniprot ID   A0AA96SDG7
Organism   Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1677386..1688627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRV97_RS07840 (RRV97_07840) pilF 1680626..1682305 (+) 1680 WP_315611200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RRV97_RS07845 (RRV97_07845) pilG 1682386..1683627 (+) 1242 WP_315611201.1 type II secretion system F family protein Machinery gene
  RRV97_RS07850 (RRV97_07850) pilD 1683629..1684516 (+) 888 WP_315611202.1 A24 family peptidase Machinery gene
  RRV97_RS07855 (RRV97_07855) coaE 1684516..1685136 (+) 621 WP_315611203.1 dephospho-CoA kinase -
  RRV97_RS07860 (RRV97_07860) yacG 1685133..1685315 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  RRV97_RS07865 (RRV97_07865) glyQ 1685397..1686296 (+) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  RRV97_RS07870 (RRV97_07870) lspA 1686445..1686951 (+) 507 WP_049331903.1 signal peptidase II -
  RRV97_RS07875 (RRV97_07875) ispH 1686965..1687933 (+) 969 WP_049331909.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45992.14 Da        Isoelectric Point: 9.9459

>NTDB_id=886642 RRV97_RS07845 WP_315611201.1 1682386..1683627(+) (pilG) [Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055]
MAQAEQKRSLFGSRSKGKRFTFEGKNIETERLVRGEVVAKDEEEARKKLQRRGIRPLRISKVKTARKRRITQEDITVFTR
QLATMMKAGLPLMQAFEIVARGHSNPSMTEMLMQVRSDVEQGSALGKSFSKYPKYFDRFYCNLVSAGESGGVLESLLDKL
AVYKEKTQAIKKKVKTALTYPIAIIVVAIALIFIMMMFVLPAFKEVYANMGAELPSLTQLVMSLSDLFVDYGWIMIILLI
VSVFGLYKLHEKSPTFQKRIDALVLRLPVFGTIVRKATIARWARTTSTLFAAGVPLVEVLDSVAGASGNILYEEATQDIR
AKVTQGLSLTSSMQSTDMFPNMVIQMAAIGEESGSLDDMLNKAAEFYEDEVDNSVTRLSHLMEPIIMVVLGSLIGILLIA
MYLPLFNLGNVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=886642 RRV97_RS07845 WP_315611201.1 1682386..1683627(+) (pilG) [Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055]
ATGGCTCAAGCAGAGCAAAAAAGAAGTCTGTTCGGTTCAAGAAGCAAAGGTAAGCGTTTTACATTTGAAGGAAAAAACAT
CGAGACCGAACGTCTTGTCCGCGGCGAAGTAGTTGCCAAAGATGAAGAAGAGGCGCGCAAAAAACTCCAGCGCAGAGGCA
TTCGCCCTTTGCGTATCAGCAAAGTCAAAACCGCACGCAAACGCCGTATTACCCAAGAAGACATCACAGTGTTTACGCGC
CAACTGGCAACGATGATGAAGGCAGGCTTGCCGCTGATGCAGGCTTTCGAAATCGTTGCTCGCGGCCACTCCAATCCGAG
CATGACCGAAATGCTGATGCAGGTTCGCTCCGATGTCGAACAAGGTAGCGCATTGGGCAAATCGTTCTCCAAATATCCAA
AATATTTCGACCGTTTCTATTGCAATCTGGTCAGCGCAGGCGAGTCCGGCGGCGTACTGGAAAGCCTATTGGACAAACTG
GCTGTCTATAAAGAAAAAACCCAAGCCATTAAGAAAAAAGTCAAAACCGCACTGACCTACCCGATTGCCATTATCGTGGT
GGCGATTGCGCTTATCTTCATCATGATGATGTTTGTACTGCCTGCCTTTAAAGAAGTTTATGCCAATATGGGCGCAGAGC
TGCCAAGCCTTACCCAACTCGTCATGAGCTTGTCCGATTTATTTGTCGACTACGGCTGGATTATGATCATCCTTTTGATT
GTTTCTGTCTTCGGCCTGTACAAACTCCATGAAAAATCGCCTACCTTCCAAAAACGAATCGATGCTCTGGTTTTGAGACT
CCCTGTTTTTGGCACCATCGTCCGAAAAGCAACCATCGCCCGTTGGGCGCGTACGACCTCTACCCTCTTCGCAGCCGGCG
TTCCTTTGGTGGAAGTATTAGATTCAGTTGCAGGCGCATCCGGCAATATTCTCTATGAAGAAGCCACTCAAGACATCCGC
GCCAAGGTAACCCAAGGTCTGTCGCTGACATCCAGTATGCAAAGTACAGATATGTTCCCCAACATGGTGATCCAAATGGC
GGCCATCGGCGAAGAGTCAGGCTCTTTGGATGATATGCTGAACAAAGCAGCCGAATTCTACGAAGATGAAGTAGACAACT
CCGTCACCCGATTGTCTCACTTGATGGAGCCTATCATCATGGTAGTATTGGGTTCGCTCATCGGTATTTTGCTGATTGCC
ATGTATCTGCCGCTGTTTAACTTGGGTAATGTCGTAGGTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

72.03

97.821

0.705
  pilG Neisseria meningitidis 44/76-A

71.04

97.821

0.695
  pilC Legionella pneumophila strain ERS1305867

46.599

96.126

0.448
  pilC Acinetobacter baumannii D1279779

46.231

96.368

0.446
  pilC Acinetobacter baylyi ADP1

45.98

96.368

0.443
  pilC Pseudomonas stutzeri DSM 10701

45.75

96.852

0.443
  pilC Vibrio cholerae strain A1552

38.5

96.852

0.373
  pilC Thermus thermophilus HB27

38.363

94.673

0.363