Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   RRV97_RS07840 Genome accession   NZ_CP135440
Coordinates   1680626..1682305 (+) Length   559 a.a.
NCBI ID   WP_315611200.1    Uniprot ID   A0AA96SC21
Organism   Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1675626..1687305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRV97_RS07830 (RRV97_07830) - 1675704..1676963 (+) 1260 WP_049322554.1 endonuclease NucS -
  RRV97_RS07835 (RRV97_07835) carB 1677003..1680218 (+) 3216 WP_315611199.1 carbamoyl-phosphate synthase large subunit -
  RRV97_RS07840 (RRV97_07840) pilF 1680626..1682305 (+) 1680 WP_315611200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RRV97_RS07845 (RRV97_07845) pilG 1682386..1683627 (+) 1242 WP_315611201.1 type II secretion system F family protein Machinery gene
  RRV97_RS07850 (RRV97_07850) pilD 1683629..1684516 (+) 888 WP_315611202.1 A24 family peptidase Machinery gene
  RRV97_RS07855 (RRV97_07855) coaE 1684516..1685136 (+) 621 WP_315611203.1 dephospho-CoA kinase -
  RRV97_RS07860 (RRV97_07860) yacG 1685133..1685315 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  RRV97_RS07865 (RRV97_07865) glyQ 1685397..1686296 (+) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  RRV97_RS07870 (RRV97_07870) lspA 1686445..1686951 (+) 507 WP_049331903.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62309.55 Da        Isoelectric Point: 6.2015

>NTDB_id=886641 RRV97_RS07840 WP_315611200.1 1680626..1682305(+) (pilF) [Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCVPIFKRGRKVYFAVSDPTQIQNFQKIAFSSGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINEEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTISLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFATALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQNWKL
YRPVGCDSCRGKGFKGRIGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=886641 RRV97_RS07840 WP_315611200.1 1680626..1682305(+) (pilF) [Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAAACCATCTCAAATCAACAAGCGGAACATTACAATAGCCTTTT
ACAATCGGGTAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTAGTTGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCACTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCGTTCCAATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACTCAAATCCA
AAACTTCCAAAAAATCGCTTTTTCTTCAGGTTTAAGTGTTGATTTGGTAGTGGTTCCCGATGACCAACTCAGCTCCTTGC
TGGAATGGTTGGGTCAGCGTTCAACAACCATTCTGAAAGAAATCAACGAAGAACATGAAGCAACGCAGCAATCTCAATCG
CTTTATATCGATAATGAAGAGGCTGAAGACGGCCCTATCCCACGCTTTATCCACAAAACCTTATCCGATGCCCTAAACGC
AGGCGCATCGGACATCCACTTTGAATTCTACGAGCAAATGGCGCGCGTCCGTTTCCGCGTGGACGGACAGTTGCGCGAAG
TCGTACAGCCGCCTGTCGCCGTACGCGGTCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCTGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCACAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
AACGCTGTTTGGCGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGCTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGCCCTTACGGCATGGTGTTAGTTACCGGTCCGACAGGCTCG
GGCAAAACCATTTCGCTCTATACCTGCCTGAGCATTTTGAACACGGAAGATGTCAACATTTCAACGGCAGAGGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGCCTGACGTTTGCCACCGCACTCCGATCCT
TCTTACGTCAAGATCCTGATATTATTATGGTCGGTGAGATCCGCGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCGACCCTGCATACCAATAATGCACCGGCCACGCTCTCCCGCCTTTTGAACATGGGTGT
CGCTCCTTTCAATATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTGGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAAATTGGAAGCTC
TACCGCCCGGTTGGTTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCATCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACCTGCGTCGCGCCGGCCTCATGAAAGTCATGCAGGGCCTGACTTCGTTGGAAGAAGTAACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

76.923

100

0.769
  pilB Acinetobacter baylyi ADP1

50.528

100

0.513
  pilB Acinetobacter baumannii D1279779

49.466

100

0.497
  pilB Legionella pneumophila strain ERS1305867

47.35

100

0.479
  pilB Vibrio cholerae strain A1552

46.277

100

0.467
  pilB Vibrio campbellii strain DS40M4

43.915

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

46.341

95.349

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

98.39

0.379
  pilF Thermus thermophilus HB27

38.361

96.064

0.369