Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   RLO20_RS00695 Genome accession   NZ_CP134538
Coordinates   119023..119961 (+) Length   312 a.a.
NCBI ID   WP_012678810.1    Uniprot ID   A0A0G7A195
Organism   Streptococcus equi subsp. equi strain XJ5012     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114023..124961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RLO20_RS00675 (RLO20_00675) - 114180..115226 (+) 1047 WP_042356681.1 acyltransferase family protein -
  RLO20_RS00680 (RLO20_00680) - 115287..115652 (+) 366 WP_012514777.1 DUF1033 family protein -
  RLO20_RS00685 (RLO20_00685) - 116189..118192 (+) 2004 WP_012678808.1 DUF1430 domain-containing protein -
  RLO20_RS00690 (RLO20_00690) - 118194..118841 (+) 648 WP_012678809.1 ATP-binding cassette domain-containing protein -
  RLO20_RS00695 (RLO20_00695) comGA/cglA 119023..119961 (+) 939 WP_012678810.1 competence type IV pilus ATPase ComGA Machinery gene
  RLO20_RS00700 (RLO20_00700) comYB 119894..120928 (+) 1035 WP_012678811.1 competence type IV pilus assembly protein ComGB Machinery gene
  RLO20_RS00705 (RLO20_00705) comGC/cglC 120929..121252 (+) 324 WP_012514782.1 competence type IV pilus major pilin ComGC Machinery gene
  RLO20_RS00710 (RLO20_00710) comGD 121230..121655 (+) 426 WP_012678812.1 competence type IV pilus minor pilin ComGD -
  RLO20_RS00715 (RLO20_00715) comGE 121612..121902 (+) 291 WP_012678813.1 competence type IV pilus minor pilin ComGE -
  RLO20_RS00720 (RLO20_00720) comYF 121889..122323 (+) 435 WP_012678814.1 competence type IV pilus minor pilin ComGF Machinery gene
  RLO20_RS00725 (RLO20_00725) comGG 122301..122663 (+) 363 WP_012678815.1 competence type IV pilus minor pilin ComGG -
  RLO20_RS00730 (RLO20_00730) - 122729..124071 (+) 1343 WP_165626751.1 IS3 family transposase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35397.61 Da        Isoelectric Point: 6.8990

>NTDB_id=880829 RLO20_RS00695 WP_012678810.1 119023..119961(+) (comGA/cglA) [Streptococcus equi subsp. equi strain XJ5012]
MIQELAKRLIRQAEELQAQDIYILPRGTGYELLMRVGDDRRLIDVCESDRMANLISHFKFVAGMNVGEKRRCQLGACDYD
LDDTKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFDGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVAYQRFISGGALIDCAQKEFEHHQANRWNQQIDQLLAEGHLNTRQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=880829 RLO20_RS00695 WP_012678810.1 119023..119961(+) (comGA/cglA) [Streptococcus equi subsp. equi strain XJ5012]
ATGATTCAAGAATTAGCCAAGCGGCTTATCAGGCAGGCTGAAGAATTGCAGGCTCAGGATATTTATATACTACCAAGGGG
GACAGGCTATGAGCTTTTGATGAGGGTAGGTGATGATAGGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGAATGTGGGCGAAAAAAGACGCTGTCAGCTCGGTGCTTGTGACTACGAC
CTTGATGACACTAAGGTGTCTCTACGATTATCAGCTGTGGGTGATTATCAGGGACAGGAGAGCTTGGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTGATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCTAGAGGCCTTT
ACCTCTTTTCTGGACCGGTGGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATAAAAAATGACCAGCTCCTGCAATTACAGGTTAACGATAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTTTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATTAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTAACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGCGTTTAC
GCTAGACTGCTAGAATTAGGCATTTCTAGGGCAGAATTGGATAATAGTCTAGCAATGGTTGCTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCACAAAAGGAATTTGAACATCATCAAGCCAACCGGTGGAATCAGCAGATTGATCAGC
TTCTTGCGGAGGGACATCTCAATACCAGGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G7A195

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.742

99.359

0.673
  comYA Streptococcus mutans UA159

63.023

99.679

0.628
  comYA Streptococcus mutans UA140

63.023

99.679

0.628
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.613

99.359

0.612
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.29

99.359

0.609
  comYA Streptococcus gordonii str. Challis substr. CH1

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.29

99.359

0.609
  comGA Lactococcus lactis subsp. cremoris KW2

52.412

99.679

0.522
  comGA Latilactobacillus sakei subsp. sakei 23K

40.767

91.987

0.375