Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHO14_RS03015 Genome accession   NZ_CP133967
Coordinates   683461..684621 (-) Length   386 a.a.
NCBI ID   WP_392552847.1    Uniprot ID   -
Organism   Orbus wheelerorum strain lpD04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 678461..689621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHO14_RS02985 (RHO14_02970) tsaD 678757..679776 (-) 1020 WP_392552841.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  RHO14_RS02990 (RHO14_02975) yacG 679779..679997 (-) 219 WP_392552842.1 DNA gyrase inhibitor YacG -
  RHO14_RS02995 (RHO14_02980) zapD 680039..680788 (-) 750 WP_392552843.1 cell division protein ZapD -
  RHO14_RS03000 (RHO14_02985) coaE 680802..681407 (-) 606 WP_392552844.1 dephospho-CoA kinase -
  RHO14_RS03005 (RHO14_02990) - 681499..682263 (-) 765 WP_392552845.1 prepilin peptidase -
  RHO14_RS03010 (RHO14_02995) hofC 682275..683468 (-) 1194 WP_392552846.1 protein transport protein HofC -
  RHO14_RS03015 (RHO14_03000) pilB 683461..684621 (-) 1161 WP_392552847.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  RHO14_RS03020 (RHO14_03005) ppdD 684627..685079 (-) 453 WP_392552848.1 prepilin peptidase-dependent pilin -
  RHO14_RS03025 (RHO14_03010) ampD 685266..685799 (+) 534 WP_392553567.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RHO14_RS03030 (RHO14_03015) - 685888..686208 (+) 321 WP_392552849.1 YqcC family protein -
  RHO14_RS03035 (RHO14_03020) - 686198..687607 (-) 1410 WP_392552850.1 sodium:proton antiporter -
  RHO14_RS03040 (RHO14_03025) ndk 687830..688258 (+) 429 WP_392552851.1 nucleoside-diphosphate kinase -
  RHO14_RS03045 (RHO14_03030) raiA 688313..688639 (-) 327 WP_392552852.1 ribosome-associated translation inhibitor RaiA -
  RHO14_RS03050 (RHO14_03035) purN 688916..689557 (-) 642 WP_392553568.1 phosphoribosylglycinamide formyltransferase -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 43430.53 Da        Isoelectric Point: 6.6572

>NTDB_id=878053 RHO14_RS03015 WP_392552847.1 683461..684621(-) (pilB) [Orbus wheelerorum strain lpD04]
MTKYSDLEIVELVNQLLNDALVKRASDIHFEPYQDLYRIRMRIDGVLQPIAILSTLLVKPLTVRLKIMANLNIAEQRLPQ
DGQLMIDKHTMRISTIPIIDGEKIVLRIMEDQQDTFSLEQLGLSENDQFHYRQALNNPQGLILVTGPTGSGKTITLYSGL
KELNESSRNICSVEDPVEMPIIGINQTQVNPKAGLDFAITLRSFLRQDPDIIMIGEIRDHETAQISVQAAQTGHLVLSTL
HTNSSAEALIRLNQMGIKNYLIASSLKLIIAQRLARKLCHHCKVIADEKFVIEEYGKRLLYPHYLAKGCQHCIGGYYGRI
GIYELLIITPQIQALLAENDQFSLNNIVQIMKKYEINTLDLSGIALVKQGITSLAEIQRVLGLINE

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=878053 RHO14_RS03015 WP_392552847.1 683461..684621(-) (pilB) [Orbus wheelerorum strain lpD04]
ATGACAAAATATAGCGATCTCGAAATTGTAGAGCTAGTTAATCAACTTTTAAACGATGCGCTTGTTAAGCGCGCATCAGA
TATTCACTTTGAGCCTTATCAAGACCTATACCGAATTAGGATGCGAATTGATGGCGTTTTACAACCAATTGCGATCTTAT
CAACTTTATTAGTAAAACCGTTAACTGTTCGCTTAAAAATAATGGCAAATCTAAATATTGCAGAGCAACGTTTGCCTCAA
GATGGACAACTGATGATAGATAAGCACACGATGCGCATATCAACGATCCCAATTATTGATGGCGAAAAAATTGTTTTACG
AATAATGGAGGATCAGCAAGATACATTCTCGCTTGAACAACTGGGTTTATCAGAAAATGATCAATTTCATTATCGGCAAG
CTTTAAATAATCCTCAAGGACTTATTTTAGTTACCGGCCCAACTGGTAGTGGTAAAACGATTACGCTTTATAGTGGGCTT
AAAGAGCTAAATGAAAGTTCACGCAATATTTGTAGTGTTGAAGATCCAGTTGAAATGCCGATTATAGGCATAAACCAAAC
ACAAGTTAATCCAAAGGCTGGTCTTGATTTTGCTATAACCTTGCGCTCATTTTTAAGACAAGATCCAGATATCATTATGA
TTGGCGAAATTCGCGATCATGAAACAGCTCAAATTTCAGTACAAGCAGCGCAAACAGGCCATTTAGTCTTATCAACATTA
CATACTAACTCTAGTGCGGAAGCATTAATTCGCCTCAATCAGATGGGTATAAAGAATTATTTAATTGCTTCTAGTTTAAA
GCTTATCATCGCACAGCGACTAGCCAGAAAGCTCTGTCATCATTGTAAAGTAATTGCCGATGAAAAATTTGTAATTGAAG
AGTACGGTAAACGCCTACTTTACCCACATTATTTAGCTAAGGGCTGTCAGCACTGTATTGGTGGATATTATGGGCGAATA
GGCATTTATGAGTTACTCATAATTACCCCACAAATTCAAGCCTTACTGGCGGAAAATGACCAATTTTCATTAAATAATAT
TGTGCAAATTATGAAAAAATATGAGATTAATACTCTTGATTTATCTGGCATTGCGTTAGTTAAACAAGGCATAACCTCGT
TAGCTGAAATACAGCGCGTGTTAGGTTTAATAAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

49.61

99.741

0.495
  pilB Legionella pneumophila strain ERS1305867

49.223

100

0.492
  pilB Acinetobacter baylyi ADP1

48.338

100

0.49
  pilB Acinetobacter baumannii D1279779

48.958

99.482

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

49.468

97.409

0.482
  pilB Vibrio campbellii strain DS40M4

49.202

97.409

0.479
  pilB Haemophilus influenzae Rd KW20

46.073

98.964

0.456
  pilB Haemophilus influenzae 86-028NP

45.288

98.964

0.448
  pilB Glaesserella parasuis strain SC1401

44.301

100

0.443
  pilF Neisseria gonorrhoeae MS11

43.557

100

0.438
  pilF Thermus thermophilus HB27

43.194

98.964

0.427
  pilB/pilB1 Synechocystis sp. PCC 6803

40.452

100

0.417
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.682

98.705

0.402
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.741

97.927

0.399