Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHO11_RS06685 Genome accession   NZ_CP133958
Coordinates   1423154..1424317 (+) Length   387 a.a.
NCBI ID   WP_392560632.1    Uniprot ID   -
Organism   Orbus mooreae strain BiB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1418154..1429317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHO11_RS06655 (RHO11_06635) - 1418201..1418812 (-) 612 WP_392560627.1 hypothetical protein -
  RHO11_RS06665 (RHO11_06645) - 1420239..1421648 (+) 1410 WP_392560628.1 sodium:proton antiporter -
  RHO11_RS06670 (RHO11_06650) - 1421638..1421958 (-) 321 WP_392560629.1 YqcC family protein -
  RHO11_RS06675 (RHO11_06655) ampD 1421977..1422516 (-) 540 WP_392560630.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RHO11_RS06680 (RHO11_06660) ppdD 1422696..1423148 (+) 453 WP_392560631.1 prepilin peptidase-dependent pilin -
  RHO11_RS06685 (RHO11_06665) pilB 1423154..1424317 (+) 1164 WP_392560632.1 GspE/PulE family protein Machinery gene
  RHO11_RS06690 (RHO11_06670) hofC 1424319..1425512 (+) 1194 WP_392560633.1 protein transport protein HofC -
  RHO11_RS06695 (RHO11_06675) - 1425525..1426295 (+) 771 WP_392560634.1 prepilin peptidase -
  RHO11_RS06700 (RHO11_06680) coaE 1426388..1426999 (+) 612 WP_392560635.1 dephospho-CoA kinase -
  RHO11_RS06705 (RHO11_06685) - 1427006..1427752 (+) 747 WP_392560636.1 cell division protein ZapD -
  RHO11_RS06710 (RHO11_06690) yacG 1427790..1428008 (+) 219 WP_392560637.1 DNA gyrase inhibitor YacG -
  RHO11_RS06715 (RHO11_06695) tsaD 1428011..1429045 (+) 1035 WP_392560638.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43115.81 Da        Isoelectric Point: 7.9469

>NTDB_id=878006 RHO11_RS06685 WP_392560632.1 1423154..1424317(+) (pilB) [Orbus mooreae strain BiB]
MLNTNIKYHESDVIEQVNQLLNDGLSQRASDIHFEPYEQQLRIRMRIDGVLKTVERLPITLAKPLIVRLKIMAKLNIAEQ
RLPQDGLLTIGQQTMRVSSIPILHGEKIVLRVMDNHKLVLSIEQLGLTNQEQNQYRKILNSPQGLILVTGPTGSGKTITL
YSGLKEINNDTRNICSVEDPIEMPIDGINQTQINTKAGLGFSSILRSFLRQDPDIMMIGEIRDNETAEIAMQAAQTGHLV
LSTLHTNSSAEALIRLNQIGIKNYLIASSLKLIIAQRLVRKLCNHCKMIADVKHNAQHYVISPNSCHFIAQGCSQCSNGY
SGRIGVYEFLLVNQQLQALLLSNSECSLNQIEQIIQQNNIKTLSSSGKELVTQGITSIAEVQRVLGD

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=878006 RHO11_RS06685 WP_392560632.1 1423154..1424317(+) (pilB) [Orbus mooreae strain BiB]
ATGCTAAACACCAATATCAAATATCATGAAAGTGATGTCATTGAACAGGTTAATCAATTACTCAATGATGGACTTAGTCA
ACGCGCCTCAGATATTCACTTTGAACCTTATGAGCAACAATTACGTATTAGAATGCGTATTGATGGCGTATTAAAAACAG
TTGAGAGACTACCAATAACTTTAGCTAAGCCATTAATTGTTAGATTAAAAATCATGGCTAAACTCAATATTGCAGAACAA
CGTTTACCACAAGACGGTTTACTAACTATCGGCCAACAGACAATGCGAGTATCAAGTATTCCTATTTTGCATGGTGAAAA
GATTGTCTTACGAGTTATGGATAATCATAAATTAGTACTCTCAATAGAGCAGCTAGGTTTGACCAATCAAGAACAGAATC
AATATCGTAAGATATTGAATAGCCCACAAGGATTAATTTTAGTAACCGGGCCGACAGGTAGCGGAAAAACGATTACGCTC
TATAGTGGTTTGAAAGAGATTAACAATGATACCCGTAATATTTGCAGCGTTGAAGATCCTATTGAAATGCCTATTGATGG
TATTAATCAAACACAAATCAATACAAAAGCAGGACTTGGTTTTAGTAGTATTTTACGCTCATTTTTAAGACAAGATCCCG
ATATTATGATGATCGGTGAAATACGTGATAATGAAACAGCTGAAATCGCAATGCAAGCAGCTCAAACAGGACATCTGGTT
TTATCAACGTTACATACAAATTCAAGTGCAGAAGCGCTAATTCGACTCAACCAAATCGGTATAAAAAATTATTTGATTGC
ATCTAGCCTAAAATTAATTATTGCTCAACGTTTAGTTAGAAAGTTATGTAACCATTGCAAAATGATTGCAGATGTAAAAC
ATAATGCTCAACACTATGTTATATCTCCAAACAGTTGCCATTTTATTGCTCAAGGTTGTAGTCAATGTAGCAACGGCTAC
TCTGGCCGTATCGGAGTATATGAATTTTTATTAGTCAACCAACAATTGCAAGCACTACTGCTAAGCAATAGCGAATGTTC
CTTAAATCAAATTGAACAGATAATCCAACAAAACAATATAAAAACATTATCCTCATCAGGAAAAGAGTTAGTTACGCAAG
GCATCACCTCTATAGCTGAAGTACAACGAGTATTAGGAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(20-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

50.128

100

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

49.742

100

0.499
  pilB Vibrio campbellii strain DS40M4

49.227

100

0.494
  pilB Legionella pneumophila strain ERS1305867

48.843

100

0.491
  pilB Haemophilus influenzae Rd KW20

46.335

98.708

0.457
  pilB Haemophilus influenzae 86-028NP

45.833

99.225

0.455
  pilB Glaesserella parasuis strain SC1401

45.646

97.933

0.447
  pilB Acinetobacter baumannii D1279779

43.669

100

0.437
  pilB Acinetobacter baylyi ADP1

43.523

99.742

0.434
  pilB/pilB1 Synechocystis sp. PCC 6803

40.447

100

0.421
  pilF Neisseria gonorrhoeae MS11

41.117

100

0.419
  pilF Thermus thermophilus HB27

39.744

100

0.401
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.052

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.12

98.966

0.377