Detailed information NTG0876881

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RF668_RS06420
• Genome accession: NZ_CP133787
• Coordinates: 1233943..1234599 (+)
• Length: 218 a.a.
• NCBI ID: WP_043735588.1
• Uniprot ID: A0AAX4AL53
• Organism: Lactococcus cremoris strain KCKM 0438
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1228943..1239599

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RF668_RS06395 (RF668_06395)	-	1229062..1230240 (+)	1179	WP_021037692.1	SLC13 family permease	-
RF668_RS06400 (RF668_06400)	-	1230414..1230974 (+)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
RF668_RS06405 (RF668_06405)	-	1231091..1231489 (+)	399	WP_043735595.1	hypothetical protein	-
RF668_RS06410 (RF668_06410)	-	1231595..1232527 (+)	933	WP_309559527.1	ABC transporter ATP-binding protein	-
RF668_RS06415 (RF668_06415)	-	1232524..1233885 (+)	1362	WP_043735590.1	ABC transporter permease	-
RF668_RS06420 (RF668_06420)	comEA	1233943..1234599 (+)	657	WP_043735588.1	helix-hairpin-helix domain-containing protein	Machinery gene
RF668_RS06425 (RF668_06425)	comEC	1234580..1236790 (+)	2211	WP_043735587.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RF668_RS06430 (RF668_06430)	-	1237074..1237850 (+)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
RF668_RS06435 (RF668_06435)	-	1238033..1238248 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
RF668_RS06440 (RF668_06440)	atpB	1238295..1239008 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
RF668_RS06445 (RF668_06445)	atpF	1239023..1239529 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23801.97 Da
• Isoelectric Point: 5.1730

>NTDB_id=876881 RF668_RS06420 WP_043735588.1 1233943..1234599(+) (comEA) [Lactococcus cremoris strain KCKM 0438]
MEKILDKFKEYWKIMLLVICALIAGGIFYIFTNSPKPAETLSVENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQISTTSGPKININ
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 657 bp

>NTDB_id=876881 RF668_RS06420 WP_043735588.1 1233943..1234599(+) (comEA) [Lactococcus cremoris strain KCKM 0438]
ATGGAAAAAATTTTAGATAAATTCAAAGAATACTGGAAAATAATGCTTTTAGTTATTTGTGCACTTATTGCTGGTGGAAT
TTTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAGTAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCCAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAATTTCAACGACAAGTGGTCCAAAAATAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-126)

• Helix-hairpin-helix motif (HHH_3) (154-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	98.624	100	0.986
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.85	100	0.436
comEA/celA/cilE	Streptococcus mitis SK321	41.85	100	0.436
comEA/celA/cilE	Streptococcus pneumoniae R6	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.991	100	0.417
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.088	100	0.417
comEA	Latilactobacillus sakei subsp. sakei 23K	38.528	100	0.408
comEA	Staphylococcus aureus MW2	34.483	100	0.367
comEA	Staphylococcus aureus N315	34.498	100	0.362