Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RHM68_RS21360 Genome accession   NZ_CP133782
Coordinates   4725384..4726601 (+) Length   405 a.a.
NCBI ID   WP_322218914.1    Uniprot ID   -
Organism   Pseudomonas sp. DC1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4720384..4731601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM68_RS21340 (RHM68_21340) - 4721819..4722115 (+) 297 WP_322218902.1 DUF2845 domain-containing protein -
  RHM68_RS21345 (RHM68_21345) - 4722330..4722683 (-) 354 WP_322218905.1 BON domain-containing protein -
  RHM68_RS21350 (RHM68_21350) - 4723039..4723455 (-) 417 WP_322218909.1 pilin -
  RHM68_RS21355 (RHM68_21355) pilB 4723681..4725381 (+) 1701 WP_322218912.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM68_RS21360 (RHM68_21360) pilC 4725384..4726601 (+) 1218 WP_322218914.1 type II secretion system F family protein Machinery gene
  RHM68_RS21365 (RHM68_21365) pilD 4726603..4727475 (+) 873 WP_322218916.1 A24 family peptidase Machinery gene
  RHM68_RS21370 (RHM68_21370) coaE 4727472..4728095 (+) 624 WP_322218919.1 dephospho-CoA kinase -
  RHM68_RS21375 (RHM68_21375) yacG 4728092..4728292 (+) 201 WP_322218922.1 DNA gyrase inhibitor YacG -
  RHM68_RS21380 (RHM68_21380) - 4728302..4728517 (-) 216 WP_201198044.1 hypothetical protein -
  RHM68_RS21385 (RHM68_21385) - 4728581..4729270 (-) 690 WP_322218924.1 energy-coupling factor ABC transporter permease -
  RHM68_RS21390 (RHM68_21390) - 4729409..4730035 (+) 627 WP_322218926.1 DUF1780 domain-containing protein -
  RHM68_RS21395 (RHM68_21395) - 4730032..4730559 (+) 528 WP_322218928.1 MOSC domain-containing protein -
  RHM68_RS21400 (RHM68_21400) - 4730611..4730784 (+) 174 WP_008028730.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44274.31 Da        Isoelectric Point: 10.1198

>NTDB_id=876722 RHM68_RS21360 WP_322218914.1 4725384..4726601(+) (pilC) [Pseudomonas sp. DC1.2]
MAAKAAKISVYAWEGTDKKGVKVTGELSGLSPALIKAQLRKQGINPGKVRKKSMSIFSLGKRIKAQEIALFTRQMATMMK
AGVPLLQSFEIIGEGFDNPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ERLKAKVKKAMTYPLAVVFVALIVTGILLIKVVPQFQSVFAGFGASLPAFTVMVIGISEFMQQWWWLMLGALIAAIFGVR
RAFKKSQAFRDWTDTWLLKLPLVGTLMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGVFPNMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=876722 RHM68_RS21360 WP_322218914.1 4725384..4726601(+) (pilC) [Pseudomonas sp. DC1.2]
ATGGCCGCCAAGGCAGCAAAAATCAGCGTGTACGCGTGGGAAGGCACGGACAAAAAAGGCGTGAAGGTGACCGGCGAATT
GAGCGGTCTGAGCCCTGCGCTGATCAAGGCGCAACTGCGCAAGCAAGGCATTAACCCAGGCAAGGTGCGCAAAAAATCCA
TGTCGATTTTCAGCCTGGGCAAACGCATCAAAGCCCAGGAGATCGCCCTGTTCACCCGGCAAATGGCGACCATGATGAAA
GCCGGCGTGCCGTTATTGCAGTCGTTCGAAATCATCGGCGAAGGCTTCGATAACCCGGCCATGCGCAAACTGGTGGACGA
GGTGAAACAAGAAGTCGCCGCAGGGAACAGCTTCGCTACGGCCCTGCGCAAGAAGCCGCAGTATTTCGACGAGTTGTACT
GCAACCTGGTGGACGCCGGGGAGCAGGCTGGCGCGTTGGATACGCTGCTGGAACGGGTAGCTACCTATAAGGAAAAAAGC
GAACGCCTGAAGGCCAAGGTCAAGAAAGCCATGACCTACCCATTGGCAGTGGTGTTCGTCGCGCTCATCGTGACGGGCAT
TCTGTTGATCAAAGTGGTGCCACAGTTCCAGTCGGTGTTCGCCGGATTTGGCGCCTCACTGCCCGCGTTCACGGTGATGG
TCATCGGTATCTCGGAGTTCATGCAGCAATGGTGGTGGCTGATGCTCGGCGCTCTGATCGCGGCGATTTTCGGGGTACGC
CGTGCGTTCAAAAAGTCGCAGGCTTTTCGCGACTGGACGGACACCTGGCTGTTGAAACTGCCCTTGGTCGGCACGTTGAT
GTACAAGTCTGCCGTGGCGCGTTACGCACGCACGTTATCGACGACATTTGCGGCCGGTGTCCCGCTGGTAGAAGCCTTGG
AGTCGGTGGCCGGCGCAACGGGCAACATCGTGTTCAAGCGTGCAGTGCTGCGCATCAAACAGGACGTCTCGACCGGCATG
CAACTGAACTTCTCTATGCGCACCTCCGGGGTCTTTCCGAACATGGCGATCCAGATGACTGCGATTGGCGAGGAGTCCGG
CGCCCTGGACGACATGCTCGATAAGGTCGCCAGTTTCTATGAGGACGAAGTGGACAACATGGTCGACAACCTGACCAGCC
TGATGGAGCCGTTCATCATGGTGGTACTGGGTGTTATTGTCGGCGGCCTGGTGGTTGCCATGTACCTGCCTATCTTCCAA
CTTGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

58.911

99.753

0.588
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio cholerae strain A1552

42.716

100

0.427
  pilC Vibrio campbellii strain DS40M4

40.887

100

0.41
  pilC Thermus thermophilus HB27

38.213

99.506

0.38