Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RG643_RS28360 Genome accession   NZ_CP133753
Coordinates   6037228..6038445 (-) Length   405 a.a.
NCBI ID   WP_044061094.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZYPA187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6032228..6043445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG643_RS28315 (RG643_28310) - 6032410..6032859 (-) 450 WP_003161439.1 MOSC domain-containing protein -
  RG643_RS28320 (RG643_28315) - 6032856..6033485 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  RG643_RS28325 (RG643_28320) - 6033618..6034043 (+) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  RG643_RS28330 (RG643_28325) - 6034040..6034510 (+) 471 WP_023103507.1 hypothetical protein -
  RG643_RS28335 (RG643_28330) - 6034507..6035196 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  RG643_RS28340 (RG643_28335) - 6035302..6035511 (+) 210 WP_003120946.1 hypothetical protein -
  RG643_RS28345 (RG643_28340) yacG 6035548..6035748 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RG643_RS28350 (RG643_28345) coaE 6035745..6036356 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  RG643_RS28355 (RG643_28350) pilD 6036353..6037225 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RG643_RS28360 (RG643_28355) pilC 6037228..6038445 (-) 1218 WP_044061094.1 type II secretion system F family protein Machinery gene
  RG643_RS28365 (RG643_28360) pilB 6038449..6040149 (-) 1701 WP_309543269.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RG643_RS28370 (RG643_28365) - 6040380..6040853 (+) 474 WP_309543270.1 pilin -
  RG643_RS28380 (RG643_28375) nadC 6041006..6041854 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44268.27 Da        Isoelectric Point: 9.8988

>NTDB_id=876323 RG643_RS28360 WP_044061094.1 6037228..6038445(-) (pilC) [Pseudomonas aeruginosa strain ZYPA187]
MADKALKTCVFVWEGTDKKGAKVKGELAGQNTMLVKAQLRKQGINPLKVRKKGITLLGKGKRVKPMDIALFTRQMATMMG
AGVPLLQSFDIISEGFDNPNMRKLVDEIKQEVSAGNSLANSLRKKPLYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVVLVAIIVSAILLIKVVPQFQSVFSSFGAELPAFTMMVINLSNLLQEWWLVVLIGLFSASFAIK
ELHKRSVNFRNTVDRYMLKIPIIGGILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVSKIKQDVSTGM
QLNFSMRTTNVFPNMAIQMTAIGEESGSLDDMLGKVAAFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=876323 RG643_RS28360 WP_044061094.1 6037228..6038445(-) (pilC) [Pseudomonas aeruginosa strain ZYPA187]
ATGGCGGATAAAGCGTTAAAAACCTGTGTTTTCGTCTGGGAAGGCACGGACAAGAAAGGGGCAAAGGTCAAGGGGGAGTT
GGCTGGCCAGAACACTATGCTGGTGAAGGCGCAACTGCGCAAACAGGGCATAAATCCGCTCAAGGTCAGAAAAAAGGGCA
TTACCCTTCTCGGCAAGGGAAAGCGCGTAAAGCCGATGGACATCGCACTATTTACTCGTCAGATGGCAACCATGATGGGT
GCTGGGGTTCCTCTCTTGCAATCGTTCGACATAATTTCCGAAGGTTTCGACAATCCCAACATGCGTAAGCTGGTCGACGA
GATCAAACAAGAGGTCTCGGCGGGTAACAGCCTGGCAAACTCCTTGCGAAAGAAACCTCTTTATTTTGACGATCTCTATT
GCAACCTGGTGGATGCAGGTGAACAATCTGGCGCCTTGGAGACTTTGCTTGACCGTGTTGCCACCTATAAGGAAAAAACC
GAATCGCTAAAAGCCAAGATAAAAAAGGCTATGACATACCCCATTGCCGTCGTCTTGGTCGCGATCATTGTTTCAGCCAT
TCTCCTGATCAAGGTCGTACCCCAGTTCCAATCCGTTTTCTCCAGTTTCGGAGCGGAACTACCAGCCTTTACAATGATGG
TGATAAACCTGTCAAATCTTTTACAGGAATGGTGGCTAGTCGTCCTCATCGGATTATTCAGCGCCAGCTTCGCCATCAAG
GAATTGCATAAGCGTTCAGTAAATTTCCGTAACACGGTAGACCGCTATATGTTGAAAATACCGATAATCGGAGGAATACT
TTACAAATCAGCCGTGGCACGTTACGCCCGTACACTTTCAACAACTTTCGCCGCAGGTGTTCCATTGGTGGAGGCACTAG
ACTCTGTCTCTGGAGCGACAGGAAACGTGGTATTCCGCAACGCGGTAAGCAAGATCAAACAGGATGTATCCACAGGTATG
CAATTGAACTTCTCCATGAGAACGACCAATGTATTCCCCAACATGGCAATCCAGATGACAGCCATCGGTGAGGAATCCGG
TTCTCTGGACGACATGTTGGGCAAGGTGGCTGCGTTCTATGAGGAGGAAGTAGACAATGCCGTCGACAATCTGACAACTC
TCATGGAACCTATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baumannii D1279779

58.911

99.753

0.588
  pilC Acinetobacter baylyi ADP1

59.045

98.272

0.58
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria meningitidis 44/76-A

44.279

99.259

0.44
  pilG Neisseria gonorrhoeae MS11

44.03

99.259

0.437
  pilC Vibrio cholerae strain A1552

43.457

100

0.435
  pilC Vibrio campbellii strain DS40M4

43.182

97.778

0.422
  pilC Thermus thermophilus HB27

37.406

99.012

0.37