Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG643_RS28365 Genome accession   NZ_CP133753
Coordinates   6038449..6040149 (-) Length   566 a.a.
NCBI ID   WP_309543269.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZYPA187     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6033449..6045149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG643_RS28325 (RG643_28320) - 6033618..6034043 (+) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  RG643_RS28330 (RG643_28325) - 6034040..6034510 (+) 471 WP_023103507.1 hypothetical protein -
  RG643_RS28335 (RG643_28330) - 6034507..6035196 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  RG643_RS28340 (RG643_28335) - 6035302..6035511 (+) 210 WP_003120946.1 hypothetical protein -
  RG643_RS28345 (RG643_28340) yacG 6035548..6035748 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RG643_RS28350 (RG643_28345) coaE 6035745..6036356 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  RG643_RS28355 (RG643_28350) pilD 6036353..6037225 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RG643_RS28360 (RG643_28355) pilC 6037228..6038445 (-) 1218 WP_044061094.1 type II secretion system F family protein Machinery gene
  RG643_RS28365 (RG643_28360) pilB 6038449..6040149 (-) 1701 WP_309543269.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RG643_RS28370 (RG643_28365) - 6040380..6040853 (+) 474 WP_309543270.1 pilin -
  RG643_RS28380 (RG643_28375) nadC 6041006..6041854 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG643_RS28385 (RG643_28380) - 6042140..6044395 (+) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  RG643_RS28390 (RG643_28385) ampD 6044546..6045112 (+) 567 WP_003116245.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62359.17 Da        Isoelectric Point: 5.7055

>NTDB_id=876324 RG643_RS28365 WP_309543269.1 6038449..6040149(-) (pilB) [Pseudomonas aeruginosa strain ZYPA187]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYSAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=876324 RG643_RS28365 WP_309543269.1 6038449..6040149(-) (pilB) [Pseudomonas aeruginosa strain ZYPA187]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACTCGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.692

100

0.583
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

50.887

99.647

0.507
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50.088

99.823

0.5
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.563

93.463

0.36