Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   RFN65_RS11730 Genome accession   NZ_CP133703
Coordinates   2309344..2310594 (-) Length   416 a.a.
NCBI ID   WP_003231847.1    Uniprot ID   A0A199WIX6
Organism   Bacillus subtilis strain CP35     
Function   unknown (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2285578..2310594 2309344..2310594 within 0


Gene organization within MGE regions


Location: 2285578..2310594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS11610 (RFN65_11610) - 2285578..2286033 (-) 456 WP_396123279.1 phage terminase small subunit P27 family -
  RFN65_RS11615 (RFN65_11615) - 2286415..2286789 (-) 375 WP_045384479.1 HNH endonuclease signature motif containing protein -
  RFN65_RS11620 (RFN65_11620) - 2286786..2287202 (-) 417 WP_100506580.1 hypothetical protein -
  RFN65_RS11625 (RFN65_11625) - 2287528..2288292 (-) 765 WP_100506579.1 hypothetical protein -
  RFN65_RS11630 (RFN65_11630) - 2288959..2289297 (-) 339 WP_251217917.1 hypothetical protein -
  RFN65_RS11635 (RFN65_11635) - 2289530..2290072 (-) 543 WP_100506578.1 tyrosine-type recombinase/integrase -
  RFN65_RS11640 (RFN65_11640) - 2290072..2290452 (-) 381 WP_285840562.1 ArpU family phage packaging/lysis transcriptional regulator -
  RFN65_RS11645 (RFN65_11645) - 2290754..2291503 (-) 750 WP_157799635.1 hypothetical protein -
  RFN65_RS11650 (RFN65_11650) - 2291775..2292137 (+) 363 WP_100506576.1 Ig-like domain-containing protein -
  RFN65_RS11655 (RFN65_11655) - 2292181..2292468 (-) 288 WP_100506575.1 YrzE family protein -
  RFN65_RS11660 (RFN65_11660) - 2292652..2292977 (-) 326 Protein_2299 VWA domain-containing protein -
  RFN65_RS11665 (RFN65_11665) - 2293020..2293217 (-) 198 WP_100506573.1 XtrA/YqaO family protein -
  RFN65_RS11670 (RFN65_11670) mutL 2293596..2295479 (-) 1884 WP_100506572.1 DNA mismatch repair endonuclease MutL -
  RFN65_RS11675 (RFN65_11675) mutS 2295495..2298071 (-) 2577 WP_033883742.1 DNA mismatch repair protein MutS -
  RFN65_RS11680 (RFN65_11680) cotE 2298204..2298749 (-) 546 WP_003231833.1 outer spore coat protein CotE -
  RFN65_RS11685 (RFN65_11685) ricA 2299011..2299442 (-) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA -
  RFN65_RS11690 (RFN65_11690) miaB 2299444..2300973 (-) 1530 WP_015483238.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  RFN65_RS11695 (RFN65_11695) kbl 2301121..2302299 (-) 1179 WP_015383694.1 glycine C-acetyltransferase -
  RFN65_RS11700 (RFN65_11700) tdh 2302312..2303355 (-) 1044 WP_015483237.1 L-threonine 3-dehydrogenase -
  RFN65_RS11705 (RFN65_11705) spoVS 2303621..2303881 (-) 261 WP_003154135.1 stage V sporulation protein SpoVS -
  RFN65_RS11710 (RFN65_11710) ymdB 2304081..2304875 (-) 795 WP_003245138.1 2',3'-cyclic-nucleotide 2'-phosphodiesterase -
  RFN65_RS11715 (RFN65_11715) rny 2304944..2306506 (-) 1563 WP_003221010.1 ribonuclease Y -
  RFN65_RS11720 (RFN65_11720) pbpX 2306782..2307957 (-) 1176 WP_063335370.1 serine hydrolase -
  RFN65_RS11725 (RFN65_11725) recA 2308125..2309171 (-) 1047 WP_003245789.1 recombinase RecA Machinery gene
  RFN65_RS11730 (RFN65_11730) cinA 2309344..2310594 (-) 1251 WP_003231847.1 competence/damage-inducible protein A Machinery gene

Sequence

Protein


Download         Length: 416 a.a.        Molecular weight: 45537.85 Da        Isoelectric Point: 5.3737

>NTDB_id=875839 RFN65_RS11730 WP_003231847.1 2309344..2310594(-) (cinA) [Bacillus subtilis strain CP35]
MEFPKKAEIIAVGSELLLGQIANTNAQFISKQLAEIGVNVFYHTAVGDNPERLKQVIRIAEERSDFIIFSGGLGPTKDDL
TKETIANTLGRPLVLNDEAFQSIEDYFKRTKRTMSPNNRKQALVIEGSDVLANHFGMAPGMLTEHESRYYMLLPGPPSEL
RPMFENEAKPLLLKKMGSNEKIVSTVLRFFGIGESQLEADLEDIIDAQTNPTIAPLAADGEVTLRLTAKHADEKETERLL
KETEAVILERVGEFFYGYDDTSLVKELSIACKEKGITISAAESFTGGLFSEWLTDHSGASKLFAGGVVCYTNDVKQNVLG
VKKETLDRFGAVSKECASELAKGVQKLTGSDIGISFTGVAGPDAQEGHEPGHVFIGISANGKEEVHEFHFAGSRTGIRKR
GAKYGCHLILKLLEQK

Nucleotide


Download         Length: 1251 bp        

>NTDB_id=875839 RFN65_RS11730 WP_003231847.1 2309344..2310594(-) (cinA) [Bacillus subtilis strain CP35]
ATGGAATTTCCAAAAAAAGCAGAAATAATTGCAGTCGGTTCAGAGCTGCTGCTTGGCCAAATCGCCAATACAAATGCTCA
ATTTATCAGCAAACAGCTCGCTGAAATCGGGGTCAACGTATTTTATCATACAGCGGTCGGCGATAACCCGGAGCGGCTGA
AGCAGGTCATCCGCATTGCTGAAGAACGCTCTGACTTCATTATTTTTTCAGGAGGGCTTGGACCCACAAAAGATGATCTG
ACGAAAGAAACGATTGCAAATACGCTGGGACGTCCGCTTGTGTTAAATGATGAGGCATTCCAATCCATTGAGGACTATTT
CAAACGAACAAAACGCACGATGTCACCTAATAACCGAAAACAGGCGCTTGTGATCGAAGGCTCTGACGTGCTGGCGAATC
ACTTTGGAATGGCCCCGGGAATGCTCACAGAGCATGAATCGCGCTATTATATGCTTCTTCCTGGACCGCCGAGCGAATTG
CGTCCAATGTTTGAAAACGAGGCAAAGCCTCTTCTGCTGAAAAAGATGGGTTCAAATGAAAAAATTGTGTCAACCGTTCT
TCGCTTTTTCGGTATCGGTGAATCTCAGCTTGAAGCCGATTTGGAAGATATTATTGATGCGCAAACCAATCCGACAATTG
CGCCTTTGGCGGCTGATGGAGAGGTGACGCTGCGTCTGACAGCCAAACACGCCGATGAAAAGGAAACAGAGCGTCTGTTG
AAAGAAACAGAGGCCGTTATCTTAGAGCGTGTCGGTGAATTTTTCTATGGTTACGATGATACTTCTCTTGTAAAAGAGCT
ATCTATAGCGTGTAAGGAAAAAGGCATTACAATTTCTGCGGCAGAAAGCTTTACCGGGGGGCTGTTTTCTGAATGGCTGA
CGGATCATAGCGGTGCCTCAAAATTATTTGCCGGGGGCGTTGTTTGCTATACAAACGACGTGAAGCAGAATGTGCTTGGT
GTCAAGAAGGAAACATTAGATCGTTTTGGGGCGGTCAGCAAGGAGTGCGCATCCGAACTGGCTAAGGGTGTTCAAAAACT
CACTGGCAGCGATATCGGCATTAGCTTTACTGGTGTAGCAGGACCTGATGCTCAAGAAGGGCATGAGCCTGGGCATGTGT
TTATCGGCATTTCCGCAAATGGTAAAGAAGAGGTTCACGAGTTTCACTTTGCGGGCTCCAGAACGGGGATCAGAAAACGC
GGCGCTAAATACGGCTGCCATTTAATCTTAAAGCTTTTAGAGCAAAAATAA

Domains

Predicted by InterproScan.

Competence-damaged protein (CinA)

(262-412)

Probable molybdopterin binding domain (MoCF_biosynth)

(9-172)

Damage-inducible protein CinA KH domain (CinA_KH)

(184-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WIX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  cinA Streptococcus mitis NCTC 12261

45.694

100

0.459
  cinA Streptococcus mitis SK321

45.694

100

0.459
  cinA Streptococcus pneumoniae TIGR4

45.455

100

0.457
  cinA Streptococcus mutans UA159

46.005

99.279

0.457
  cinA Streptococcus pneumoniae Rx1

45.215

100

0.454
  cinA Streptococcus pneumoniae R6

45.215

100

0.454
  cinA Streptococcus pneumoniae D39

44.976

100

0.452
  cinA Streptococcus suis isolate S10

41.379

90.625

0.375