Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RBH93_RS18605 Genome accession   NZ_CP133079
Coordinates   4045151..4046260 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain HS1906     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4040151..4051260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH93_RS18575 (RBH93_18575) - 4040611..4041618 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  RBH93_RS18580 (RBH93_18580) glnK 4041877..4042215 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  RBH93_RS18585 (RBH93_18585) arfB 4042417..4042833 (+) 417 WP_111901080.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  RBH93_RS18590 (RBH93_18590) - 4042859..4043350 (+) 492 WP_139747064.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  RBH93_RS18595 (RBH93_18595) - 4043710..4044135 (-) 426 WP_005340360.1 hypothetical protein -
  RBH93_RS18600 (RBH93_18600) yaaA 4044330..4045103 (-) 774 WP_307895561.1 peroxide stress protein YaaA -
  RBH93_RS18605 (RBH93_18605) pilU 4045151..4046260 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  RBH93_RS18610 (RBH93_18610) pilT 4046289..4047323 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  RBH93_RS18615 (RBH93_18615) - 4047363..4048064 (+) 702 WP_307895562.1 YggS family pyridoxal phosphate-dependent enzyme -
  RBH93_RS18620 (RBH93_18620) proC 4048207..4049031 (+) 825 WP_307895563.1 pyrroline-5-carboxylate reductase -
  RBH93_RS18625 (RBH93_18625) - 4049043..4049594 (+) 552 WP_005341416.1 YggT family protein -
  RBH93_RS18630 (RBH93_18630) yggU 4049594..4049896 (+) 303 WP_139461355.1 DUF167 family protein YggU -
  RBH93_RS18635 (RBH93_18635) - 4049940..4050359 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=871674 RBH93_RS18605 WP_005340356.1 4045151..4046260(-) (pilU) [Aeromonas veronii strain HS1906]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=871674 RBH93_RS18605 WP_005340356.1 4045151..4046260(-) (pilU) [Aeromonas veronii strain HS1906]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGTGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCTACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACGGGACATCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GTTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGTTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGATT
GCTGATCAAGCTCTCCGGTCGCGAACGGCTCGGCACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36