Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q9L40_RS14000 Genome accession   NZ_CP132190
Coordinates   1902357..1904045 (-) Length   562 a.a.
NCBI ID   WP_044127953.1    Uniprot ID   -
Organism   Vibrio cholerae strain DRC187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1897357..1909045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L40_RS13975 (Q9L40_13975) yacG 1898423..1898620 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  Q9L40_RS13980 (Q9L40_13980) zapD 1898778..1899518 (-) 741 WP_000207203.1 cell division protein ZapD -
  Q9L40_RS13985 (Q9L40_13985) coaE 1899550..1900158 (-) 609 WP_162891880.1 dephospho-CoA kinase -
  Q9L40_RS13990 (Q9L40_13990) pilD 1900155..1901030 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  Q9L40_RS13995 (Q9L40_13995) pilC 1901088..1902314 (-) 1227 WP_305798925.1 type II secretion system F family protein Machinery gene
  Q9L40_RS14000 (Q9L40_14000) pilB 1902357..1904045 (-) 1689 WP_044127953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9L40_RS14005 (Q9L40_14005) - 1904052..1904480 (-) 429 WP_305798924.1 pilin -
  Q9L40_RS14010 (Q9L40_14010) nadC 1904725..1905615 (-) 891 WP_305798923.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q9L40_RS14015 (Q9L40_14015) ampD 1905913..1906458 (+) 546 WP_088128396.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q9L40_RS14020 (Q9L40_14020) - 1906577..1907965 (-) 1389 WP_305798922.1 IS4-like element ISVa18 family transposase -
  Q9L40_RS14025 (Q9L40_14025) fldB 1908249..1908767 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62044.16 Da        Isoelectric Point: 6.0856

>NTDB_id=867524 Q9L40_RS14000 WP_044127953.1 1902357..1904045(-) (pilB) [Vibrio cholerae strain DRC187]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=867524 Q9L40_RS14000 WP_044127953.1 1902357..1904045(-) (pilB) [Vibrio cholerae strain DRC187]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAAGT
TAGCGCTTCGGGAACGTCAGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTCACCGAACAACTGA
GCCATATTTTCGGTTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTACCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTCTCTGACCCGACCTTATT
GCAAGCTGAAGAAGAGTTTCGTTTTGCCACTGGGCTGCAAGTTGAACTGGCGCTAGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGAAAAGAGATCAGCCAAGATGAGCTCGCCACT
CTGGTTAAAGTCAGTGATGACGAACTGCAATCCATTGAAGACTTAAGCCAAGATGACTCTCCGGTTAGCCGTTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGACATTCATTTTGAGCCTTATGAAAACCAGTATCGGATCCGTC
TGCGCTGCGATGGGATCTTGGTGGAAACTCAGCAACCAGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCTGAGCGCCGTTTGCCGCAAGACGGGCGGATTAAACTGCGCCTAAGCCGAGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCTACGTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTACAAGTGCAGCCGAAAATCGGTTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCATACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAATCTGGCGTCATCACTCAGTTTAATCATCGCCCAGCGTCTTGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCATTTCAACCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50

92.883

0.464
  pilF Neisseria gonorrhoeae MS11

45.907

100

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.19

100

0.4
  pilF Thermus thermophilus HB27

39.372

96.263

0.379
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37