Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K5631_RS12560 Genome accession   NZ_AP024617
Coordinates   2831709..2832974 (+) Length   421 a.a.
NCBI ID   WP_208170502.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2826709..2837974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5631_RS12540 (TUM17386_24760) ampD 2826967..2827545 (-) 579 WP_208170503.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5631_RS12545 (TUM17386_24770) nadC 2827704..2828564 (+) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5631_RS12550 (TUM17386_24780) - 2829424..2829837 (+) 414 WP_144224956.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5631_RS12555 (TUM17386_24790) pilB 2829847..2831553 (+) 1707 WP_144224957.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5631_RS12560 (TUM17386_24800) pilC 2831709..2832974 (+) 1266 WP_208170502.1 type II secretion system F family protein Machinery gene
  K5631_RS12565 (TUM17386_24810) pilD 2833050..2833964 (+) 915 WP_208170501.1 A24 family peptidase Machinery gene
  K5631_RS12570 (TUM17386_24820) coaE 2833965..2834576 (+) 612 WP_025012056.1 dephospho-CoA kinase -
  K5631_RS12575 (TUM17386_24830) zapD 2834573..2835313 (+) 741 WP_028779562.1 cell division protein ZapD -
  K5631_RS12580 (TUM17386_24840) yacG 2835358..2835570 (+) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5631_RS12585 (TUM17386_24850) mutT 2835570..2835968 (+) 399 WP_044733562.1 8-oxo-dGTP diphosphatase MutT -
  K5631_RS12590 - 2836046..2836319 (+) 274 Protein_2442 transposase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 45854.93 Da        Isoelectric Point: 10.2778

>NTDB_id=86658 K5631_RS12560 WP_208170502.1 2831709..2832974(+) (pilC) [Shewanella algae strain TUM17386]
MATATATAKNNKSKNKNAKAQPKIHTFTWKGHNRDGKLSSGELRGTSITEIKTLLKSQGINPKTVRKKSASMFGDKKIKP
MDIAVITRQIATMLAAGVPLVTSIELIARGHEKNRVRVLLGTVLADLQAGIPLSDALRPHRVYFDDLYVDLVAAGEHSGS
LDAVFDRIATYREKSEALKSKIKKAMFYPAAVVVVAIAVTVLLLVAVVPQFEEIFNGFGAELPAFTQLIVNISRGLQSTW
YLFLAGIVISVWLFVRAHRNSEHFRNRIDEMVLKIPAIGDILHKGAMARFARTLATTFAAGVPLIDGLESAAGASGNYVY
RKALLNVRTEVMAGMQMNVAMRTTKLFPDMLIQMVMIGEESGSLDNMLNKVANIYEMQVDDAVDGLSSLIEPIMMVVIGT
IVGGLIVGMYLPIFNLGKVVG

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=86658 K5631_RS12560 WP_208170502.1 2831709..2832974(+) (pilC) [Shewanella algae strain TUM17386]
ATGGCAACTGCAACGGCGACAGCAAAAAACAATAAAAGCAAGAACAAAAACGCTAAAGCCCAGCCCAAGATTCACACCTT
TACCTGGAAGGGGCATAATCGCGACGGCAAACTCAGCTCAGGCGAACTGCGTGGTACCAGCATTACTGAGATAAAAACCC
TTCTTAAGAGCCAGGGGATTAATCCCAAGACGGTACGTAAAAAATCAGCTTCCATGTTTGGCGATAAAAAGATCAAGCCG
ATGGATATCGCGGTGATCACCCGTCAGATAGCCACTATGCTGGCTGCGGGTGTCCCCTTGGTAACCTCTATTGAATTGAT
CGCCCGCGGCCATGAGAAAAACAGAGTGCGGGTGCTGCTTGGCACTGTATTGGCCGATCTCCAGGCCGGTATTCCACTCT
CCGATGCCCTCCGCCCACACAGAGTCTATTTTGACGACCTCTATGTCGACTTGGTGGCCGCCGGTGAACATTCAGGTTCC
TTGGATGCCGTATTTGATCGTATCGCCACCTATAGGGAAAAGTCAGAGGCCCTTAAGTCGAAAATCAAGAAAGCCATGTT
TTACCCTGCTGCAGTGGTGGTAGTTGCCATAGCCGTTACCGTGCTGCTGCTTGTTGCCGTTGTACCTCAATTTGAAGAAA
TCTTTAACGGATTTGGCGCCGAACTGCCTGCCTTTACCCAATTAATTGTCAATATCAGCCGCGGCCTTCAGAGTACCTGG
TATCTATTTCTAGCTGGTATCGTTATCAGTGTCTGGCTCTTTGTCCGTGCTCACCGCAACTCTGAGCACTTCCGTAACCG
TATCGACGAAATGGTGCTTAAGATCCCCGCGATAGGAGATATCTTACATAAAGGTGCTATGGCCCGTTTTGCCCGCACTC
TGGCCACCACTTTTGCCGCCGGGGTGCCGCTGATTGATGGCCTGGAGTCTGCCGCCGGCGCCTCCGGTAATTATGTTTAC
CGCAAAGCGCTGTTGAATGTACGTACCGAAGTAATGGCCGGGATGCAGATGAATGTGGCCATGCGTACCACCAAGCTTTT
TCCCGATATGCTAATCCAGATGGTGATGATAGGTGAAGAGTCCGGCTCATTGGATAATATGCTCAACAAAGTTGCCAATA
TCTATGAGATGCAGGTAGACGATGCCGTAGATGGCCTTTCCAGCCTGATAGAACCTATTATGATGGTAGTAATCGGCACT
ATAGTCGGCGGCCTAATCGTCGGCATGTATCTACCTATCTTCAACTTGGGTAAAGTGGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(290-412)

Type II secretion system (T2SS), protein F (T2SSF)

(87-209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.138

94.774

0.523
  pilC Acinetobacter baylyi ADP1

49.507

96.437

0.477
  pilC Legionella pneumophila strain ERS1305867

48.762

95.962

0.468
  pilC Acinetobacter baumannii D1279779

47.304

96.912

0.458
  pilC Vibrio cholerae strain A1552

46.384

95.249

0.442
  pilC Vibrio campbellii strain DS40M4

44.913

95.724

0.43
  pilG Neisseria meningitidis 44/76-A

42.5

95.012

0.404
  pilG Neisseria gonorrhoeae MS11

42.5

95.012

0.404

Multiple sequence alignment