Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K5627_RS02250 Genome accession   NZ_AP024616
Coordinates   480375..481649 (-) Length   424 a.a.
NCBI ID   WP_221059354.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17384     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475375..486649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5627_RS02220 - 477042..477309 (-) 268 Protein_412 transposase -
  K5627_RS02225 (TUM17384_04180) mutT 477381..477779 (-) 399 WP_219031309.1 8-oxo-dGTP diphosphatase MutT -
  K5627_RS02230 (TUM17384_04190) yacG 477779..477991 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5627_RS02235 (TUM17384_04200) zapD 478036..478776 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5627_RS02240 (TUM17384_04210) coaE 478773..479384 (-) 612 WP_054744929.1 dephospho-CoA kinase -
  K5627_RS02245 (TUM17384_04220) pilD 479385..480299 (-) 915 WP_144164451.1 A24 family peptidase Machinery gene
  K5627_RS02250 (TUM17384_04230) pilC 480375..481649 (-) 1275 WP_221059354.1 type II secretion system F family protein Machinery gene
  K5627_RS02255 (TUM17384_04240) pilB 481810..483516 (-) 1707 WP_221059731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5627_RS02260 (TUM17384_04250) - 483530..483991 (-) 462 WP_221059355.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5627_RS02265 (TUM17384_04260) nadC 484858..485718 (-) 861 WP_096142088.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5627_RS02270 (TUM17384_04270) ampD 485877..486455 (+) 579 WP_221059356.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 46021.23 Da        Isoelectric Point: 10.2645

>NTDB_id=86622 K5627_RS02250 WP_221059354.1 480375..481649(-) (pilC) [Shewanella algae strain TUM17384]
MATATATAPAKSNKAKNKGAKAQPKIHTFTWKGQNRDGKLSSGELRGTSITEIKTLLKSQGINPKTVRKKSTSLLGGDKK
IKPMDIAVITRQIATMLAAGVPLVTSIELIARGHEKNKVRVLLGTVLADVQAGIPLSDALRPHRVYFDDLYVDLVAAGEH
SGSLDAVFDRIATYREKSEALKSKIKKAMFYPAAVVVVAMAVTALLLVVVVPQFEEIFNGFGAELPAFTQLIVSISRALQ
STWYFFLVGVVISIWLFIRAHRNSENFRNRIDEMVLKIPLIGEILHKGAMARFARTLATTFAAGVPLIDGLESAAGASGN
YVYRKALLNVRTEVMAGMQMNVAMRTTKLFPDMLIQMVMIGEESGSLDNMLNKVANIYEMQVDDAVDGLSSLIEPIMMVV
IGTIVGGLIVGMYLPIFNLGKVVG

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=86622 K5627_RS02250 WP_221059354.1 480375..481649(-) (pilC) [Shewanella algae strain TUM17384]
ATGGCAACCGCAACCGCAACGGCACCAGCAAAAAGCAATAAAGCCAAAAACAAAGGCGCCAAAGCCCAACCCAAGATCCA
CACCTTTACCTGGAAAGGGCAGAATCGCGATGGCAAACTCAGCTCCGGCGAACTGCGTGGCACCAGCATCACAGAGATTA
AAACCCTGCTTAAGAGCCAGGGGATCAATCCCAAGACGGTACGTAAAAAATCAACATCTCTTTTAGGAGGTGATAAAAAA
ATTAAGCCGATGGATATCGCGGTGATCACCCGTCAGATAGCCACTATGTTGGCTGCCGGCGTACCGCTGGTCACTTCTAT
CGAGCTGATCGCCCGCGGCCATGAAAAAAACAAGGTGCGGGTGCTGCTTGGCACTGTATTGGCCGACGTCCAGGCCGGTA
TTCCACTCTCCGATGCACTTCGTCCCCACAGAGTCTATTTCGATGACCTCTATGTCGACTTGGTTGCCGCCGGTGAACAT
TCAGGCTCCTTGGATGCAGTATTTGACCGTATCGCCACCTATAGGGAAAAGTCAGAAGCCCTTAAGTCAAAAATCAAAAA
AGCCATGTTCTACCCAGCGGCTGTAGTCGTCGTAGCCATGGCCGTAACCGCCTTACTACTAGTGGTGGTGGTTCCTCAGT
TTGAAGAAATCTTTAATGGCTTTGGTGCCGAACTGCCAGCTTTCACTCAATTGATCGTTAGTATAAGCAGAGCTCTACAA
AGTACTTGGTACTTTTTTTTAGTGGGCGTGGTCATTAGTATCTGGCTCTTTATTCGTGCTCATCGAAATTCAGAAAATTT
CCGAAATCGTATTGATGAAATGGTACTTAAGATTCCACTTATTGGTGAAATTCTCCATAAAGGTGCCATGGCCCGCTTCG
CCCGCACTCTGGCCACCACTTTTGCTGCCGGGGTGCCGCTGATTGATGGCCTGGAGTCTGCCGCCGGCGCCTCCGGTAAT
TATGTTTACCGCAAGGCGCTGTTAAATGTGCGTACCGAAGTGATGGCCGGGATGCAGATGAACGTGGCCATGCGTACCAC
CAAGCTATTCCCTGATATGCTAATCCAGATGGTGATGATAGGTGAAGAATCCGGCTCATTGGACAACATGCTCAACAAAG
TCGCCAATATCTATGAGATGCAGGTAGACGATGCCGTAGATGGCCTTTCCAGCCTAATAGAACCTATTATGATGGTAGTT
ATCGGTACCATAGTCGGCGGCTTAATCGTCGGCATGTACCTGCCTATCTTCAACTTGGGTAAAGTGGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(90-212)

Type II secretion system (T2SS), protein F (T2SSF)

(293-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.388

94.104

0.521
  pilC Acinetobacter baylyi ADP1

48.894

95.991

0.469
  pilC Legionella pneumophila strain ERS1305867

48.515

95.283

0.462
  pilC Acinetobacter baumannii D1279779

47.304

96.226

0.455
  pilC Vibrio cholerae strain A1552

46.75

94.34

0.441
  pilC Vibrio campbellii strain DS40M4

45.297

95.283

0.432
  pilG Neisseria meningitidis 44/76-A

41.787

97.642

0.408
  pilG Neisseria gonorrhoeae MS11

41.787

97.642

0.408

Multiple sequence alignment