Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5627_RS02255 Genome accession   NZ_AP024616
Coordinates   481810..483516 (-) Length   568 a.a.
NCBI ID   WP_221059731.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17384     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476810..488516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5627_RS02220 - 477042..477309 (-) 268 Protein_412 transposase -
  K5627_RS02225 (TUM17384_04180) mutT 477381..477779 (-) 399 WP_219031309.1 8-oxo-dGTP diphosphatase MutT -
  K5627_RS02230 (TUM17384_04190) yacG 477779..477991 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5627_RS02235 (TUM17384_04200) zapD 478036..478776 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5627_RS02240 (TUM17384_04210) coaE 478773..479384 (-) 612 WP_054744929.1 dephospho-CoA kinase -
  K5627_RS02245 (TUM17384_04220) pilD 479385..480299 (-) 915 WP_144164451.1 A24 family peptidase Machinery gene
  K5627_RS02250 (TUM17384_04230) pilC 480375..481649 (-) 1275 WP_221059354.1 type II secretion system F family protein Machinery gene
  K5627_RS02255 (TUM17384_04240) pilB 481810..483516 (-) 1707 WP_221059731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5627_RS02260 (TUM17384_04250) - 483530..483991 (-) 462 WP_221059355.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5627_RS02265 (TUM17384_04260) nadC 484858..485718 (-) 861 WP_096142088.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5627_RS02270 (TUM17384_04270) ampD 485877..486455 (+) 579 WP_221059356.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5627_RS02275 (TUM17384_04280) ampE 486509..487360 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  K5627_RS02280 (TUM17384_04290) pdhR 487624..488376 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63004.15 Da        Isoelectric Point: 5.2572

>NTDB_id=86623 K5627_RS02255 WP_221059731.1 481810..483516(-) (pilB) [Shewanella algae strain TUM17384]
MPTSGLHLGLSTLFIRKGLLDESSLAEAIKQSQKNKQALVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLNGLDEES
LAGIEVTDTDKRQQEESESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86623 K5627_RS02255 WP_221059731.1 481810..483516(-) (pilB) [Shewanella algae strain TUM17384]
ATGCCTACATCCGGTCTTCACTTAGGATTATCGACCCTATTTATCCGTAAAGGGCTACTCGATGAGAGTAGCCTGGCGGA
GGCCATCAAACAGTCGCAAAAGAACAAACAAGCCCTGGTCACCACTCTGGTGACCAGTCAACTTTTATCCGCCCGCACTA
TCGCCGAACTCTGTTACGAAGAGTATGGCACCCCGCTGCTGGATCTCAATGAGTTTGACTTAAGCAGTATTCCGGAAGAG
TTTCTCAACAAAAAACTGATCGAAAAGCACAGATGCCTGCCGTTATTCAAGCGTGGCAACCGCCTCTATATCGCCACCTC
AGACCCCACCAATATTGCCGCCCTGGAAGATTTTCAATTTAGTGCCGGCCTGCACGCCGAAGCGATACTGGTAGAGGAGG
ATAAGCTCAGTAAAGCACTGGAAAAAGTGCTTGAAGAAGACCTGAGTTCACTGGATCTCAATGGCCTCGATGAGGAATCC
CTCGCCGGTATCGAGGTCACCGACACAGACAAGCGTCAACAGGAAGAGAGTGAAAGTAAAGATGACGCCCCTATCGTCAT
CTATATCAATAAAATTTTGACCGATGCCATCCGTAAGGGCGCTTCGGATCTGCATTTCGAGCCCTACGAGAAACGCTACC
GCATCCGTTTTCGTATTGATGGCATCCTCCATGAGGTGTCCGAGCCACCCATTTCCCTCTCGGGCCGTATCTCAGCCCGC
TTGAAGGTGATGTCCAAACTGGATATCGCCGAGCGCCGGGTCCCTCAGGATGGCCGCATCAAGATGAAGCTGTCCCGTAA
CAAGAGTATCGATTTCCGGGTCAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTTATGCGGATCCTCGACTCCTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAAATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTGACAGGCCCTACCGGCTCTGGTAAAACCGTTTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CCGGACTGACCTTCGCTTCGGCGCTGCGCTCTTTTTTACGTCAGGATCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCTGAAATCGCCATCAAGGCGGCACAAACCGGCCACTTGGTACTCTCAACCCTGCATACCAACTCATCGGC
CGAAACCCTTACCCGCCTGCTTAATATGGGGGTACCAGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCATACAAGCCCTGCGGCTGCGAGTTCTGCTCCGGTGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAACTCACTGCAGATTGCCG
CTACCGCCAAGCAGCAAGGGATGCGCGATCTGCGCCTTTCCGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAAGTGAACCGGGTCACCAGCTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.537

98.768

0.588
  pilB Acinetobacter baumannii D1279779

58.73

99.824

0.586
  pilB Legionella pneumophila strain ERS1305867

56.537

99.648

0.563
  pilB Vibrio parahaemolyticus RIMD 2210633

53.263

99.824

0.532
  pilB Vibrio cholerae strain A1552

53.417

97.887

0.523
  pilB Vibrio campbellii strain DS40M4

51.687

99.12

0.512
  pilF Neisseria gonorrhoeae MS11

48.142

99.472

0.479
  pilF Thermus thermophilus HB27

37.943

99.296

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.225

97.183

0.371

Multiple sequence alignment