Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7612_RS04145 Genome accession   NZ_CP131722
Coordinates   799771..801165 (+) Length   464 a.a.
NCBI ID   WP_061724184.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2018C09-260     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794771..806165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7612_RS04120 (Q7612_04110) rsmE 794790..795527 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7612_RS04125 (Q7612_04115) lnt 795577..797106 (-) 1530 WP_080357035.1 apolipoprotein N-acyltransferase -
  Q7612_RS04130 (Q7612_04120) corC 797129..798028 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7612_RS04135 (Q7612_04125) ampD 798650..799210 (-) 561 WP_042599472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7612_RS04140 (Q7612_04130) pilA 799325..799774 (+) 450 WP_048954594.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7612_RS04145 (Q7612_04135) pilB 799771..801165 (+) 1395 WP_061724184.1 GspE/PulE family protein Machinery gene
  Q7612_RS04150 (Q7612_04140) pilC 801162..802379 (+) 1218 WP_061724182.1 type II secretion system F family protein Machinery gene
  Q7612_RS04155 (Q7612_04145) pilD 802376..803068 (+) 693 WP_048954597.1 A24 family peptidase Machinery gene
  Q7612_RS04160 (Q7612_04150) rho 803122..804384 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  Q7612_RS04165 (Q7612_04155) metJ 804632..804949 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  Q7612_RS04170 (Q7612_04160) cueR 804963..805349 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  Q7612_RS04175 (Q7612_04165) - 805426..805632 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  Q7612_RS04180 (Q7612_04170) - 805706..805912 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52820.29 Da        Isoelectric Point: 5.9781

>NTDB_id=864761 Q7612_RS04145 WP_061724184.1 799771..801165(+) (pilB) [Haemophilus influenzae strain 2018C09-260]
MTSYALLHTQRVTAQNGEIFTISPDLWVRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTESQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINSQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFQNLRVSGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=864761 Q7612_RS04145 WP_061724184.1 799771..801165(+) (pilB) [Haemophilus influenzae strain 2018C09-260]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCTGATTTATG
GGTGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTCCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACTGAAAGCCAACAACAGGCATTTCAACACGCTCTTAGCCAACCCCAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTTAATACACCTGATAAACACATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATTCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGAGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACAGATTTTCAAAATTTACGTGTAAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.845

100

0.978
  pilB Haemophilus influenzae Rd KW20

95.68

99.784

0.955
  pilB Glaesserella parasuis strain SC1401

57.826

99.138

0.573
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.148

100

0.416
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.557

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.853

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilF Thermus thermophilus HB27

35.714

100

0.366