Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q7612_RS04140 Genome accession   NZ_CP131722
Coordinates   799325..799774 (+) Length   149 a.a.
NCBI ID   WP_048954594.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2018C09-260     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794325..804774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7612_RS04120 (Q7612_04110) rsmE 794790..795527 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7612_RS04125 (Q7612_04115) lnt 795577..797106 (-) 1530 WP_080357035.1 apolipoprotein N-acyltransferase -
  Q7612_RS04130 (Q7612_04120) corC 797129..798028 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7612_RS04135 (Q7612_04125) ampD 798650..799210 (-) 561 WP_042599472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7612_RS04140 (Q7612_04130) pilA 799325..799774 (+) 450 WP_048954594.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7612_RS04145 (Q7612_04135) pilB 799771..801165 (+) 1395 WP_061724184.1 GspE/PulE family protein Machinery gene
  Q7612_RS04150 (Q7612_04140) pilC 801162..802379 (+) 1218 WP_061724182.1 type II secretion system F family protein Machinery gene
  Q7612_RS04155 (Q7612_04145) pilD 802376..803068 (+) 693 WP_048954597.1 A24 family peptidase Machinery gene
  Q7612_RS04160 (Q7612_04150) rho 803122..804384 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15453.90 Da        Isoelectric Point: 9.1085

>NTDB_id=864760 Q7612_RS04140 WP_048954594.1 799325..799774(+) (pilA) [Haemophilus influenzae strain 2018C09-260]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADIKTTKGYVASVITQSGGITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVKK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=864760 Q7612_RS04140 WP_048954594.1 799325..799774(+) (pilA) [Haemophilus influenzae strain 2018C09-260]
ATGAAATTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATCCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCA
GCGGATATAAAGACAACAAAAGGCTATGTAGCCTCAGTAATCACTCAATCAGGTGGCATTACCGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCAAAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Haemophilus influenzae 86-028NP

90.476

98.658

0.893
  pilA Glaesserella parasuis strain SC1401

55.405

99.329

0.55
  pilA2 Legionella pneumophila str. Paris

41.429

93.96

0.389
  pilA Vibrio campbellii strain DS40M4

40.426

94.631

0.383
  pilA2 Legionella pneumophila strain ERS1305867

37.838

99.329

0.376