Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7616_RS04085 Genome accession   NZ_CP131717
Coordinates   787043..788437 (+) Length   464 a.a.
NCBI ID   WP_342120463.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2016N21-284     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782043..793437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7616_RS04060 (Q7616_04065) rsmE 782057..782794 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7616_RS04065 (Q7616_04070) lnt 782844..784373 (-) 1530 WP_118806752.1 apolipoprotein N-acyltransferase -
  Q7616_RS04070 (Q7616_04075) corC 784396..785295 (-) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7616_RS04075 (Q7616_04080) ampD 785928..786482 (-) 555 WP_050848915.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7616_RS04080 (Q7616_04085) pilA 786597..787046 (+) 450 WP_342120462.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7616_RS04085 (Q7616_04090) pilB 787043..788437 (+) 1395 WP_342120463.1 GspE/PulE family protein Machinery gene
  Q7616_RS04090 (Q7616_04095) pilC 788434..789654 (+) 1221 WP_342120465.1 type II secretion system F family protein Machinery gene
  Q7616_RS04095 (Q7616_04100) pilD 789651..790343 (+) 693 WP_105894637.1 A24 family peptidase Machinery gene
  Q7616_RS04100 (Q7616_04105) rho 790397..791659 (-) 1263 WP_112102455.1 transcription termination factor Rho -
  Q7616_RS04105 (Q7616_04110) metJ 791907..792224 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  Q7616_RS04110 (Q7616_04115) cueR 792238..792624 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  Q7616_RS04115 (Q7616_04120) - 792701..792907 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  Q7616_RS04120 (Q7616_04125) - 792981..793187 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52909.37 Da        Isoelectric Point: 5.8789

>NTDB_id=864617 Q7616_RS04085 WP_342120463.1 787043..788437(+) (pilB) [Haemophilus influenzae strain 2016N21-284]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYRGRIGVYQFLHWQQNGYQTDFKNLRESGLEKVSQGITDGKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=864617 Q7616_RS04085 WP_342120463.1 787043..788437(+) (pilB) [Haemophilus influenzae strain 2016N21-284]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTTTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCAGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAATTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAACACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTAGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGCCAAGATCCCGATATTATTATGCTGGGCGAAATTCGCGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCCCAGACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCCCGCTTACAACAACTCG
GTATTCAACAGTATGAAATTGAAAACAGTTTACTACTCGTCATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGGAA
AAGAAATTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.06

100

0.981
  pilB Haemophilus influenzae Rd KW20

96.76

99.784

0.966
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.877

100

0.403
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

36.345

100

0.373