Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q7616_RS04080 Genome accession   NZ_CP131717
Coordinates   786597..787046 (+) Length   149 a.a.
NCBI ID   WP_342120462.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2016N21-284     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 781597..792046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7616_RS04060 (Q7616_04065) rsmE 782057..782794 (-) 738 WP_005662982.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7616_RS04065 (Q7616_04070) lnt 782844..784373 (-) 1530 WP_118806752.1 apolipoprotein N-acyltransferase -
  Q7616_RS04070 (Q7616_04075) corC 784396..785295 (-) 900 WP_005662987.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7616_RS04075 (Q7616_04080) ampD 785928..786482 (-) 555 WP_050848915.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7616_RS04080 (Q7616_04085) pilA 786597..787046 (+) 450 WP_342120462.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  Q7616_RS04085 (Q7616_04090) pilB 787043..788437 (+) 1395 WP_342120463.1 GspE/PulE family protein Machinery gene
  Q7616_RS04090 (Q7616_04095) pilC 788434..789654 (+) 1221 WP_342120465.1 type II secretion system F family protein Machinery gene
  Q7616_RS04095 (Q7616_04100) pilD 789651..790343 (+) 693 WP_105894637.1 A24 family peptidase Machinery gene
  Q7616_RS04100 (Q7616_04105) rho 790397..791659 (-) 1263 WP_112102455.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15496.88 Da        Isoelectric Point: 8.4623

>NTDB_id=864616 Q7616_RS04080 WP_342120462.1 786597..787046(+) (pilA) [Haemophilus influenzae strain 2016N21-284]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTSCTGGKNGIA
ADIKTAKGYVASVITQSGGITVKGNGTLANMEYILQATGNAAAGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=864616 Q7616_RS04080 WP_342120462.1 786597..787046(+) (pilA) [Haemophilus influenzae strain 2016N21-284]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACCATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCGTCTGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAGCTGTACAGGGGGAAAAAATGGTATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTTATCACTCAATCAGGTGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCTGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

97.987

100

0.98
  pilA Haemophilus influenzae 86-028NP

93.96

100

0.94
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

42.446

93.289

0.396
  pilA2 Legionella pneumophila strain ERS1305867

41.727

93.289

0.389
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376