Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   WDR08_RS18270 Genome accession   NZ_CP148035
Coordinates   3838356..3838820 (+) Length   154 a.a.
NCBI ID   WP_057386486.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain S1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3833356..3843820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDR08_RS18245 (WDR08_18245) yacG 3833526..3833726 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  WDR08_RS18250 (WDR08_18250) coaE 3833723..3834334 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  WDR08_RS18255 (WDR08_18255) pilD 3834331..3835203 (-) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  WDR08_RS18260 (WDR08_18260) pilC 3835204..3836421 (-) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  WDR08_RS18265 (WDR08_18265) pilB 3836425..3838125 (-) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WDR08_RS18270 (WDR08_18270) pilA/pilAI 3838356..3838820 (+) 465 WP_057386486.1 pilin Machinery gene
  WDR08_RS18275 (WDR08_18275) - 3838978..3840291 (+) 1314 WP_223697243.1 O-antigen ligase family protein -
  WDR08_RS18285 (WDR08_18285) nadC 3840472..3841320 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16220.53 Da        Isoelectric Point: 8.4551

>NTDB_id=863771 WDR08_RS18270 WP_057386486.1 3838356..3838820(+) (pilA/pilAI) [Pseudomonas aeruginosa strain S1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKEQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=863771 WDR08_RS18270 WP_057386486.1 3838356..3838820(+) (pilA/pilAI) [Pseudomonas aeruginosa strain S1]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGAGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.132

98.701

0.396
  pilA Vibrio cholerae strain A1552

40.132

98.701

0.396
  pilA Vibrio cholerae C6706

40.132

98.701

0.396
  pilA2 Legionella pneumophila strain ERS1305867

39.189

96.104

0.377
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364