Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI216_RS08615 Genome accession   NZ_AP024595
Coordinates   1815161..1816348 (-) Length   395 a.a.
NCBI ID   WP_005009695.1    Uniprot ID   N9DPW5
Organism   Acinetobacter bouvetii strain JCM 18991     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1810161..1821348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI216_RS08590 (ACBO_16650) - 1810539..1810988 (+) 450 WP_005009707.1 MaoC family dehydratase -
  KI216_RS08595 (ACBO_16660) - 1811163..1812191 (+) 1029 WP_005009705.1 helix-turn-helix transcriptional regulator -
  KI216_RS08600 (ACBO_16670) gcvH 1812277..1812651 (+) 375 WP_005009703.1 glycine cleavage system protein GcvH -
  KI216_RS08605 (ACBO_16680) ygiD 1812726..1813583 (-) 858 WP_005009700.1 4,5-DOPA dioxygenase extradiol -
  KI216_RS08610 (ACBO_16690) - 1813872..1815050 (+) 1179 WP_005009697.1 MFS transporter -
  KI216_RS08615 (ACBO_16700) pilU 1815161..1816348 (-) 1188 WP_005009695.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI216_RS08620 (ACBO_16710) - 1816612..1817667 (-) 1056 WP_005009693.1 3-deoxy-7-phosphoheptulonate synthase -
  KI216_RS08625 (ACBO_16720) - 1818368..1819966 (+) 1599 WP_005009691.1 ATP-binding cassette domain-containing protein -
  KI216_RS08630 (ACBO_16730) - 1820061..1821230 (-) 1170 WP_005009688.1 fatty acid desaturase family protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 44670.04 Da        Isoelectric Point: 6.1963

>NTDB_id=86308 KI216_RS08615 WP_005009695.1 1815161..1816348(-) (pilU) [Acinetobacter bouvetii strain JCM 18991]
MYTAELLEEARRLMFHMLSKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTADKTKLFAYSLMNEKQRKEFETELEC
NFAISVPNVSRFRVNVFQQQLNVGMVIRTITNEIPNFEKLQLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
HSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGKGRRAAVEIMLNTPLVSDLILKGEFHELKAIMGKS
RELGMQTFDQALFDLYNDGAISYEEALRNADSMNELRLQIKLKSPRANPAMQNTAAEFSMQAEEKAESDDAQQAS

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=86308 KI216_RS08615 WP_005009695.1 1815161..1816348(-) (pilU) [Acinetobacter bouvetii strain JCM 18991]
ATGTACACTGCCGAATTACTGGAAGAAGCGCGCCGTTTAATGTTCCATATGCTCAGTAAAGTGGTTGAATATGGCGGTTC
AGATTTGTTTATTACGGCAGATTTTCCACCAAGCATTAAGCATCAGGGTTTAATGAAGCCTTTTGGACAGCAGGAACTGA
CAGCCGATAAAACCAAACTCTTTGCCTATAGCTTAATGAATGAGAAACAGCGTAAAGAATTTGAAACTGAACTGGAATGC
AACTTTGCTATTAGTGTGCCTAATGTATCCAGATTCCGTGTCAATGTATTTCAGCAGCAGCTGAATGTCGGCATGGTCAT
TCGAACCATTACCAATGAAATTCCAAATTTTGAAAAACTTCAGCTGCCAGAATCACTGAAAAACGTGATTATGGAAAAAC
GCGGTCTGGTGCTCGTCGTGGGCGGTACGGGTTCTGGCAAATCTACGTCTTTGGCCGCGATGATCGATTATAGAAATGAA
CACAGTGCAGGGCATATTATTACGGTTGAAGATCCTGTTGAATATGTGCATAAGCATAAAAAATCGATGATTACCCATCG
TGAAGTCGGTGTAGATTGCCATTCTTGGCACAATGCTCTAAAAAATACGCTGCGTCAGGCGCCAGATGTCATTTTGATTG
GTGAGATCCGGGATACAGAAACCATGGAGCATGCGATTGCCTTTGCTGAAACAGGCCATTTATGTTTAGGGACTCTGCAT
TCAAATAATGCCAATCAAACTTTAGACCGGATCATTAATTTCTTCCCGGAAGAGCGCCGGAATCAATTGCTTATGGATTT
ATCTTCAAATATGAAAGCGATTATTTCACAGCGCTTAATTCGGACTGAAGATGGGAAAGGGCGGCGCGCCGCAGTGGAAA
TTATGCTGAATACACCGTTAGTTTCAGACTTAATTTTAAAAGGTGAATTCCACGAGCTGAAAGCGATTATGGGCAAGTCG
CGTGAGCTGGGCATGCAGACGTTTGATCAAGCTTTATTTGATTTGTATAACGATGGCGCAATTTCATATGAAGAGGCGCT
GCGAAATGCAGACTCAATGAATGAGCTGCGTTTGCAGATTAAACTGAAAAGCCCGCGGGCAAACCCTGCGATGCAAAATA
CAGCGGCCGAATTCAGTATGCAGGCTGAAGAAAAAGCTGAAAGTGACGACGCGCAGCAGGCATCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9DPW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.345

88.101

0.532
  pilU Acinetobacter baylyi ADP1

56.145

90.633

0.509
  pilU Vibrio cholerae strain A1552

53.468

87.595

0.468
  pilT Pseudomonas aeruginosa PAK

43.114

84.557

0.365
  pilT Acinetobacter baylyi ADP1

42.857

85.063

0.365
  pilT Acinetobacter baumannii D1279779

43.333

83.544

0.362
  pilT Acinetobacter baumannii strain A118

43.333

83.544

0.362
  pilT Acinetobacter nosocomialis M2

43.333

83.544

0.362

Multiple sequence alignment