Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q5H80_RS11535 Genome accession   NZ_CP130748
Coordinates   2650900..2652585 (+) Length   561 a.a.
NCBI ID   WP_304564835.1    Uniprot ID   -
Organism   Vibrio sp. SNU_ST1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2645900..2657585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5H80_RS11515 (Q5H80_11515) pdhR 2647402..2648163 (-) 762 WP_304564832.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  Q5H80_RS11520 (Q5H80_11520) ampD 2648645..2649245 (-) 601 Protein_2218 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q5H80_RS11525 (Q5H80_11525) nadC 2649329..2650219 (+) 891 WP_139685627.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q5H80_RS11530 (Q5H80_11530) pilA 2650474..2650899 (+) 426 WP_304564834.1 pilin Machinery gene
  Q5H80_RS11535 (Q5H80_11535) pilB 2650900..2652585 (+) 1686 WP_304564835.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q5H80_RS11540 (Q5H80_11540) pilC 2652601..2653827 (+) 1227 WP_304564836.1 type II secretion system F family protein Machinery gene
  Q5H80_RS11545 (Q5H80_11545) pilD 2653896..2654765 (+) 870 WP_304564837.1 A24 family peptidase Machinery gene
  Q5H80_RS11550 (Q5H80_11550) coaE 2654768..2655382 (+) 615 WP_304564838.1 dephospho-CoA kinase -
  Q5H80_RS11555 (Q5H80_11555) zapD 2655409..2656149 (+) 741 WP_017101235.1 cell division protein ZapD -
  Q5H80_RS11560 (Q5H80_11560) yacG 2656230..2656427 (+) 198 WP_017101236.1 DNA gyrase inhibitor YacG -
  Q5H80_RS11565 (Q5H80_11565) rplS 2656874..2657227 (-) 354 WP_009847607.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62172.00 Da        Isoelectric Point: 5.3100

>NTDB_id=861301 Q5H80_RS11535 WP_304564835.1 2650900..2652585(+) (pilB) [Vibrio sp. SNU_ST1]
MLTNLPTVLRQASLLSLTQEQAVAELVHASGRSTPEALLTLGLFTGDSLAHNIKTIFGLPLVQLANTDYEVLCDQLGLRE
LITKYRAIPVVISNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMTSIEDLSKDDSPVSRFINQILVDAARKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADNSPDIFLRHSIPNNQT
LYKATPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=861301 Q5H80_RS11535 WP_304564835.1 2650900..2652585(+) (pilB) [Vibrio sp. SNU_ST1]
GTGCTAACCAACCTCCCAACCGTTCTCCGTCAGGCTAGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCAGAGCTAGT
TCATGCTTCTGGCAGGTCAACGCCTGAAGCTTTGCTCACTCTGGGCTTATTCACTGGCGATAGCCTCGCACATAACATCA
AAACCATTTTCGGTTTGCCGTTGGTGCAGCTAGCCAATACTGACTATGAAGTCTTGTGTGACCAATTAGGGCTTCGTGAA
TTGATCACTAAGTATCGTGCTATCCCAGTTGTCATCTCTAACTCTACTCTCACGCTCGCCTCTGCCGATCCAACCGACTT
GCAAGCTGAAGACGACTTTCGATTTGCAACAGGATTACAGATTGAGTTGGTTGTCGCTAACTACTCCGAATTAGAAGGCG
CCATACGAAAGCTGTATGGCCGATCTATTTCCGGGCAAGACTCCAAGCGCAAAGAGATCACCCAAGATGAGCTCGCCAAT
CTCGTTGAAGTCTCTGACGACGAAATGACTTCAATTGAAGACCTTAGCAAAGACGACTCCCCTGTTAGCCGTTTTATCAA
TCAAATATTAGTGGATGCGGCCCGTAAAGGCGCATCAGACATCCACTTCGAACCTTATGAAGAAAACTACCGAGTACGTC
TGCGTTGCGATGGGATCCTTGTTGAGGTCCAACAGCCCGCTTCTCATTTAAGCCGTCGGTTATCCGCTCGTTTAAAGATC
CTTGCAAAGCTTGATATCGCCGAACGTCGCCTGCCGCAAGACGGTCGCATTAAGCTGCGTTTGAATGACGAACTCGCGAT
CGATATGCGTGTATCGACTCTACCGACATTATGGGGAGAAAAGATCGTACTGCGCCTTCTAGACAGCAGCGCAGCCAATC
TCGACATTGATAAGCTCGGTTACAGCGAGGATCAAAAAGCGCTCTATCTCAATGCCCTAAAACGCCCGCAAGGGATGATC
TTAATGACTGGGCCCACTGGCAGCGGCAAAACGGTATCTCTTTATACGGGGCTTCGCATGCTTAACACCTCTGAGCGCAA
TATCTCAACAGCTGAAGATCCGGTGGAGATCAACCTGTGTGGTATCAATCAGGTACAGGTGACACCAAAGATTGGTTTTG
GATTTGCCGAAGCACTACGCTCTTTCTTGCGACAAGACCCTGATGTGGTGATGGTCGGTGAGATCCGTGATTTAGAAACC
GCCGAGATCGCGATCAAAGCGTCACAAACGGGTCACTTAGTCCTCTCGACACTCCATACTAACTCAGCTTCGGAAACCGT
GACTCGGCTCGCCCATATGGGGATTGAACCATTTAACCTCGCTTCGTCATTAAGCCTGATTATCGCGCAGCGACTGGCAA
GGCGACTGTGCAACCACTGTAAAGCAGCTGACAACTCGCCGGACATATTTCTGCGTCACTCTATTCCTAACAATCAAACA
CTCTACAAAGCCACCCCACAAGGGTGCAATGAGTGTAATCAGGGTTACTCCGGTCGAGTGGGTATCTACGAAGTGATGCC
GTTCACCGACAAACTAAAAAATAGTCTGATCAATAAACCTAACGCGTTAGAGATTGAAGATCTCGCGCGTAGAGAAGGAA
TGAGAACACTGCAAGAATCCGGTATCGATAAGTTGCTTGAAGGCACGACCAGCTATCAAGAACTGCAACGTGTTCTGTAT
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.002

100

0.733
  pilB Vibrio parahaemolyticus RIMD 2210633

69.929

100

0.701
  pilB Vibrio campbellii strain DS40M4

69.573

100

0.697
  pilB Legionella pneumophila strain ERS1305867

48.101

98.574

0.474
  pilB Acinetobacter baumannii D1279779

51.06

92.513

0.472
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilF Neisseria gonorrhoeae MS11

45.047

95.365

0.43
  pilF Thermus thermophilus HB27

37.591

97.683

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

34.175

100

0.362