Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6J79_RS05655 Genome accession   NZ_AP024555
Coordinates   1192077..1192538 (-) Length   153 a.a.
NCBI ID   WP_000649326.1    Uniprot ID   A0A0X1KZH2
Organism   Vibrio cholerae strain IDH-03506     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1187077..1197538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J79_RS05635 coaE 1187581..1188189 (-) 609 WP_000011557.1 dephospho-CoA kinase -
  K6J79_RS05640 pilD 1188186..1189061 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  K6J79_RS05645 pilC 1189119..1190345 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  K6J79_RS05650 pilB 1190388..1192077 (-) 1690 Protein_1042 type IV-A pilus assembly ATPase PilB -
  K6J79_RS05655 pilA 1192077..1192538 (-) 462 WP_000649326.1 pilin Machinery gene
  K6J79_RS05660 nadC 1192783..1193673 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6J79_RS05665 ampD 1193969..1194514 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6J79_RS05670 fldB 1194794..1195312 (-) 519 WP_000690112.1 flavodoxin FldB -
  K6J79_RS05675 xerD 1195450..1196358 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  K6J79_RS05680 dsbC 1196375..1197127 (+) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15722.14 Da        Isoelectric Point: 8.9860

>NTDB_id=85939 K6J79_RS05655 WP_000649326.1 1192077..1192538(-) (pilA) [Vibrio cholerae strain IDH-03506]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSAIGVGLANITALKTNIEDYIATEGSFPATTAGTAAGFT
RLGTVEDMGDGKIVIAPTASGALGGTIKYTFDAGVVSSSKIQLARDANGLWTCSTTVTSEIAPKGCTAGATIN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=85939 K6J79_RS05655 WP_000649326.1 1192077..1192538(-) (pilA) [Vibrio cholerae strain IDH-03506]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAATTATGTAAAAAAATCAGCTATTGGGGTTGGCTTAGCAAACATCACAGCAC
TGAAAACTAATATTGAAGATTATATTGCGACAGAAGGCTCTTTTCCTGCAACAACTGCAGGTACGGCCGCAGGATTTACA
CGCTTAGGTACTGTTGAAGACATGGGTGACGGTAAGATTGTAATTGCCCCAACAGCATCAGGTGCATTAGGAGGTACAAT
AAAATACACATTCGATGCTGGTGTAGTATCTAGTTCAAAAATCCAATTAGCAAGAGATGCCAATGGATTATGGACTTGTA
GTACCACCGTTACTAGTGAAATAGCACCAAAAGGCTGTACTGCAGGTGCAACAATTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1KZH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilA Vibrio cholerae strain A1552

100

100

1
  pilA Vibrio cholerae C6706

100

100

1
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.438
  pilA Acinetobacter baumannii strain A118

42.667

98.039

0.418
  pilA Vibrio parahaemolyticus RIMD 2210633

41.892

96.732

0.405
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

93.464

0.373
  pilA2 Legionella pneumophila str. Paris

39.31

94.771

0.373
  pilA2 Legionella pneumophila strain ERS1305867

38.621

94.771

0.366

Multiple sequence alignment