Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Q1W70_RS14285 Genome accession   NZ_CP130043
Coordinates   3059539..3060756 (-) Length   405 a.a.
NCBI ID   WP_302097464.1    Uniprot ID   -
Organism   Pseudomonas kielensis strain ZE23JCel16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3054539..3065756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1W70_RS14250 (Q1W70_14250) - 3055152..3056420 (+) 1269 WP_182342367.1 OprD family porin -
  Q1W70_RS14255 (Q1W70_14255) - 3056415..3056654 (-) 240 WP_166593124.1 DUF2790 domain-containing protein -
  Q1W70_RS14260 (Q1W70_14260) - 3056885..3057571 (+) 687 WP_166593125.1 energy-coupling factor ABC transporter permease -
  Q1W70_RS14265 (Q1W70_14265) - 3057635..3057850 (+) 216 WP_166593126.1 hypothetical protein -
  Q1W70_RS14270 (Q1W70_14270) yacG 3057847..3058047 (-) 201 WP_095166544.1 DNA gyrase inhibitor YacG -
  Q1W70_RS14275 (Q1W70_14275) coaE 3058044..3058667 (-) 624 WP_166593127.1 dephospho-CoA kinase -
  Q1W70_RS14280 (Q1W70_14280) pilD 3058664..3059536 (-) 873 WP_182342365.1 A24 family peptidase Machinery gene
  Q1W70_RS14285 (Q1W70_14285) pilC 3059539..3060756 (-) 1218 WP_302097464.1 type II secretion system F family protein Machinery gene
  Q1W70_RS14290 (Q1W70_14290) - 3060994..3061410 (+) 417 WP_166592405.1 pilin -
  Q1W70_RS14295 (Q1W70_14295) - 3061604..3061960 (+) 357 WP_302097465.1 BON domain-containing protein -
  Q1W70_RS14300 (Q1W70_14300) - 3062133..3065081 (-) 2949 WP_302097466.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44256.98 Da        Isoelectric Point: 10.2401

>NTDB_id=856985 Q1W70_RS14285 WP_302097464.1 3059539..3060756(-) (pilC) [Pseudomonas kielensis strain ZE23JCel16]
MPFKATKVSLYAWQGTDRTGRKMSGESSAQNPAQIKARLRQRGIIPGKVRKKFAPLFRAGQRVTAQDIAHFTRQMATLLK
AGVPLLQAFDIIAEGFDNPRMRCLLEGLKQDVMAGSSLTSALRKKPQYFDELYCNLIDAGEQAGALDTLLERVASDREKS
ERLKARMRKAMTYPLAVLLVAVIVSAILLLKVVPQFEVMFHGFGAELPALTRSVIGLAEYLRHSGWLLLGGLLAAALGVH
QAQRRSSRFRDWLQTRWLDLPIVGPLLRKSAVARYARTLATTFAAGVPLLEALDSVAGATGNRLFKQAVLMIRQEVCTGM
QLHVAMRNSGVFPNLAIQMTAIGEESGALDEMLGNVAGYYEGEVESLVDHLTSLLEPLIMGVLGVIVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=856985 Q1W70_RS14285 WP_302097464.1 3059539..3060756(-) (pilC) [Pseudomonas kielensis strain ZE23JCel16]
ATGCCGTTCAAAGCAACAAAGGTCAGCCTGTACGCCTGGCAAGGCACCGATAGAACAGGCCGCAAAATGTCCGGTGAATC
GAGCGCGCAGAATCCGGCCCAGATCAAGGCCCGGTTGCGCCAACGCGGCATCATCCCGGGCAAGGTACGGAAAAAGTTCG
CCCCCCTGTTCCGCGCAGGCCAGCGGGTCACGGCCCAGGATATCGCGCACTTCACCCGGCAAATGGCGACCCTGCTCAAG
GCCGGGGTGCCGTTGCTGCAGGCCTTCGACATCATCGCCGAAGGCTTCGATAACCCACGTATGCGTTGCCTGCTGGAGGG
CTTGAAACAGGACGTGATGGCCGGCAGCAGCCTGACCAGTGCCTTGCGCAAAAAGCCGCAATACTTCGACGAGCTCTACT
GCAACCTGATTGACGCCGGCGAACAGGCCGGCGCCCTCGACACCTTGCTGGAGCGGGTGGCGAGCGACCGGGAAAAAAGT
GAACGGCTCAAGGCGCGGATGCGCAAAGCCATGACCTACCCGCTGGCGGTGCTCCTGGTGGCCGTGATCGTCAGCGCCAT
CCTGTTACTCAAGGTGGTGCCGCAGTTCGAAGTGATGTTCCACGGTTTTGGCGCCGAACTTCCCGCACTGACCCGCAGCG
TGATCGGGCTCGCCGAATACCTGCGGCACAGCGGGTGGCTGCTGCTGGGTGGGCTGCTCGCTGCTGCGCTGGGCGTGCAC
CAGGCCCAACGCCGCTCCTCGAGGTTTCGCGACTGGCTGCAGACTCGCTGGCTGGATCTGCCAATTGTCGGCCCCCTGCT
GCGAAAGTCGGCGGTCGCTCGTTATGCCCGGACATTAGCGACCACCTTCGCCGCCGGGGTGCCGCTGCTGGAGGCCTTGG
ATTCGGTGGCCGGAGCCACCGGCAACCGGCTGTTCAAACAGGCGGTGCTGATGATTCGCCAGGAGGTGTGTACCGGCATG
CAGTTGCATGTGGCGATGCGCAACAGTGGGGTATTTCCCAACCTGGCGATCCAGATGACCGCGATTGGCGAAGAATCCGG
GGCGCTGGACGAAATGCTCGGCAACGTCGCGGGCTACTATGAAGGCGAAGTCGAGTCCCTGGTCGATCACCTGACCAGTC
TGCTGGAACCTCTTATCATGGGGGTACTTGGCGTAATCGTCGGCGGGCTGGTGGTTGCCATGTACCTGCCCATTTTCCAA
CTTGGTACGGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.235

100

0.612
  pilC Acinetobacter baylyi ADP1

53.676

100

0.541
  pilC Acinetobacter baumannii D1279779

51.724

100

0.519
  pilC Legionella pneumophila strain ERS1305867

51.263

97.778

0.501
  pilC Vibrio campbellii strain DS40M4

41.837

96.79

0.405
  pilC Vibrio cholerae strain A1552

41.327

96.79

0.4
  pilG Neisseria gonorrhoeae MS11

39.303

99.259

0.39
  pilG Neisseria meningitidis 44/76-A

39.055

99.259

0.388
  pilC Thermus thermophilus HB27

37.931

100

0.38